Osteoprotegerin(OPG)-receptor Activator of NF-kB Ligand(RANKL) 유전자 다형성과 전혈세포에서의 OPG, sRANKL 생산 사이의 연관성
The Relationship Between Osteoprotegerin(OPG)-receptor Activator of NF-kB Ligand(RANKL) Gene Polymorphism and Their Production by Whole Blood Cells
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목적:한국 폐경여성에서 OPG와 RANKL 유전자 다형성 양상과 전혈세포에서의 OPG, sRANKL 생산 사이에 연관성이 있는가를 분석하고자 하였다. 방법:자연폐경여성 267명에서 OPG T245G, G1181C 다형성양상과 RANKL rs2277438 A/G 다형성 양상을 PCR-RFLP(Polymerase Chain Reaction-Restriction Fragment Length Polymorphism)와 염기서열분석으로 분석하고 lipopolysaccharide로 자극한 전혈세포에서 혈청 OPG와 sRANKL의 생산을 측정하였다. 결과: OPG T245G, OPG G1181C 및 RANKL rs2277438 유전자 다형성 양상에 따른 전혈세포에서의 OPG와 sRANKL 생산, RANKL×1000/OPG 비는 유의한 차이가 없었다. OPG T245G와OPG G1181C 다형성으로 구성된 haplotype 유전자형에 따른 전혈세포에서의 OPG 와 sRANKL 생산량, RANKL×1000/OPG 비 또한 유의한 차이가 없었다. OPG G1181C와 RANKL rs2277438 유전자 다형성의 복합 유전자형에 따른 전혈세포에서의 OPG와 sRANKL 생산, RANKL×1000/ OPG 비도 유사한 양상을 보였다. 결론: 한국 폐경여성에서 OPG 유전자의 T245G와G1181C 다형성 및 RANKL 유전자의 rs2277438 A/G 다형성은 전혈세포에서의 OPG와 sRANKL 생산에 영향을 미치지 않는다.
Abstract
Objective: To investigate the association between polymorpjhisms of osteoprotegerin(OPG), and receptor activator of nuclear factor-[kappa]B ligand(RANKL) genes and production of OPG, and soluble RANKL (sRANKL) by whole blood cellsin Korean postmenopausal women. Methods: The OPG gene T245G, G1181C, and the RANKL gene rs2277438 polymorphisms were analyzed by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) in 267 Korean postmenopausal women. The production of OPG and sRANKL by lipopolysaccharide-stimulated whole blood cells were also measured. Results: The productions of OPG, sRANKL and the ratio of RANKL×1000/OPG by lipopolysaccharide-stimulated whole blood cells were not different according to the OPG gene T245G, G1181C, and the RANKL gene rs2277438 polymorphisms. No significant differences in the productions of OPG, sRANKL and the ratio of RANKL×1000/OPG were noted among the haplotype genotypes of OPG gene T245G and G1181C polymorphisms. There were no significant differences in the productions of OPG, sRANKL and the ratio of RANKL ×1000/OPG among combined genotypes of OPG gene G1181C and RANKL gene rs2277438 polymorphisms. Conclusion: The OPG gene T245G, G1181C, and the RANKL gene rs2277438 polymorphisms do not affect the production of OPG, and sRANKL by lipopolysaccharide-stimulated whole blood cells.