Wnt 신호전달계 내 유전자 다형성과 전혈세포에서의 Osteoprotegerin 및 Soluble Receptor Activator of NF-kB Ligand 생산 사이의 연관성
The Relationship between Wnt Signaling Pathway Gene Polymorphisms and Production of Osteoprotegerin and Soluble Receptor Activator of NF-kB by Whole Blood Cells
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목적: 한국 폐경여성에서 Wnt 신호전달계내 유전자 다형성 양상과 전혈세포에서의 osteoprotegerin (OPG) 및 soluble receptor activator of NF-kB ligand (sRANKL) 생산 사이에 연관성을 분석하고자 하였다. 방법: 자연 폐경여성 141명에서 Wnt 신호전달계내 유전자 다형성 양상을 분석하고, lipopolysaccharide로 자극한 전혈세포에서 OPG와 sRANKL의 생산을 측정하였다. 결과: LRP c266 A>G와 c3893 C>T, FZD6 c1033 A>C, axin II c148 C>T, Tcf c766 G>A 및 APC c5465 T>A 단일 다형성 및 LRP c266 A>G와 c3893 C>T 다형성으로 구성된 haplotype 유전자형에 따른 전혈세포에서의 OPG와 sRANKL 생산, RANKLx1000/OPG는 차이가 없었다. 결론: 한국 폐경여성에서 LRP c266 A>G 및 c3893 C>T, FZD6 c1033 A>C, axin II c148 C>T, Tcf c766 G>A, APC c5465 T>A 다형성은 전혈세포에서의 OPG와 sRANKL 생산에 영향을 미치지 않는다.
Abstract
Objectives: To investigate the relationship between single nucleotide polymorphisms (SNPs) in Wnt signal pathway genes, and production of osteoprotegerin (OPG) and soluble receptor activator of NF-κB ligand (sRANKL) by whole blood cells in postmenopausal Korean women. Methods: Low density lipoprotein receptor-related protein (LRP) 5 c266 A>G and c3893 C>T, frizzled (FZD) 6 c1033 A>C, axin II c148 C>T, T cell factor (Tcf) 1 c766 G>A, and adenomatous polyposis coli (APC) c5465 T>A polymorphisms were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), direct sequencing, and Taqman assay in 141 postmenopausal Korean women. The production of OPG and sRANKL by lipopolysaccharide-stimulated whole blood cells were also measured. Results: The production of OPG and sRANKL by lipopolysaccharide-stimulated whole blood cells, and their ratio of RANKLx1,000/OPG were not different according to the LRP 5 c266 A>G and c3893 C>T, FZD 6 c1033 A>C, axin II c148 C>T, Tcf 1 c766 G>A, and APC c5465 T>A polymorphisms. No significant differences in the production of OPG and sRANKL, and their ratio of RANKLx1,000/OPG were noted among combined genotypes of LRP c266 A>G and c3893 C>T polymorphisms. Conclusion: LRP 5 c266 A>G and c3893 C>T, FZD 6 c1033 A>C, axin II c148 C>T, Tcf 1 c766 G>A, and APC c5465 T>A polymorphisms do not affect the production of OPG and sRANKL by lipopolysaccharide- stimulated whole blood cells.
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