생물시험법을 이용한 중금속 물질의 급성 및 피부독성 평가
Evaluating the in vitro Acute and Dermal Toxicity of Heavy Metals
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Cadmium chloride(CdCl2), copper(II) chloride(CuCl2), potassium dichromate(VI)(K2Cr2O7), zinc chloride (ZnCl2) and nickel(II) chloride(NiCl2) are found in a wide range of products. The purpose of this study was to determine the acute toxicity and skin irritancy of the five chemicals using in vitro systems, and compare the results with in vivo endpoints. In this study, BALB/c 3T3 cells were exposed to several concentrations of CdCl2, CuCl2, K2Cr2O7 ZnCl2, and NiCl2,and the neutral red uptake (NRU) assay was used to analyze cell viability. In addition, the chemicals were applied to the EpiDerm human skin model (EPI-200) to determine which of them causes significant cytotoxicity. Compared with the UN GHS and EU risk phrases, the assay results exhibited a high prediction rate (100%) in the skin bioassay. In contrast, the acute toxicity bioassay results were more similar to the lethal dose, 50% obtained from rat experiments than GHS and R-phrases. In summation, these findings indicate that acute toxicity and skin irritancy bioassays can be utilized as an alternative to the use of animals in evaluating chemical toxicity.
Abstract
Cadmium chloride(CdCl2), copper(II) chloride(CuCl2), potassium dichromate(VI)(K2Cr2O7), zinc chloride (ZnCl2) and nickel(II) chloride(NiCl2) are found in a wide range of products. The purpose of this study was to determine the acute toxicity and skin irritancy of the five chemicals using in vitro systems, and compare the results with in vivo endpoints. In this study, BALB/c 3T3 cells were exposed to several concentrations of CdCl2, CuCl2, K2Cr2O7 ZnCl2, and NiCl2,and the neutral red uptake (NRU) assay was used to analyze cell viability. In addition, the chemicals were applied to the EpiDerm human skin model (EPI-200) to determine which of them causes significant cytotoxicity. Compared with the UN GHS and EU risk phrases, the assay results exhibited a high prediction rate (100%) in the skin bioassay. In contrast, the acute toxicity bioassay results were more similar to the lethal dose, 50% obtained from rat experiments than GHS and R-phrases. In summation, these findings indicate that acute toxicity and skin irritancy bioassays can be utilized as an alternative to the use of animals in evaluating chemical toxicity.
참고문헌 (12)
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