인간치수세포 (hDPSC) 분화개선을 위한 리튬도핑 인산삼칼슘의 합성 및 특성평가
Synthesis and characterization of Li-doped tricalcium phosphate for improving the differentiation of human dental pulp stem cells
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초록
인간 치수 세포와 Li 이온이 도핑된 β-인산삼칼슘(β-TCP)의 상호 작용과 생물학적 환경에서의 분해 거동을 조 사하였다. 이를 위해 3가지 다른 양의 리튬 이온이 첨가된 β-TCP를 공침법을 통해 합성하였다. 결정 구조의 격자 상수는 치환 부위와 이온 반경의 차이로 인해 리튬을 첨가하면 변화하였다. 리튬이온이 첨가된 β-TCP는 순수한 β-TCP에 비해 우 수한 생체 활성을 보였으며, 이는 리튬 이온이 세포 분화에 긍정적인 영향을 미친다는 것을 시사한다. 또한, 골형성 성능도 리튬 이온을 첨가될 때 더욱 향상되었다. 이는 더 나은 골 형성 활성을 달성하기 위해 중요한 미량 원소인 리튬을 β-TCP 에 첨가하는 이점을 입증할 수 있다. 결과적으로, 리튬과 β-TCP의 조합은 골 재생을 향상시키는 유망한 방법이 될 수 있으며, 이러한 결과는 인간 치수 세포에서 뼈 관련 유전자 발현을 유도함으로써 우수한 골 형성을 자극하는 효과를 가진다.
Abstract
The interactions of human dental pulp stem cell (hDPSCs) with Li-doped β-tricalcium phosphates (β-TCP) and their degradation behavior in biological environment were investigated. For this purpose, three different amounts of Li ions- doped β-TCP were synthesized by co-precipitation method. The lattice constants of crystal structure were slightly changed with addition of lithium due to the replacing site and the difference of ionic radius. Li-doped β-TCP showed significantly higher ALP activity compared to pure β-TCP, suggesting that lithium ion positively effects the cell differentiation. In addition, the osteogenic performance was also improved by the adding Li ions. These could demonstrate the benefit of incorporating an important trace elements, lithium, into β-TCP to achieve better osteogenesis activity. As a result, the combination of Li with β-TCP may be a promising method to improve bone regeneration, suggesting excellent in vitro osteogenic stimulation properties by inducing bone related gene expression in hDPSCs.
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