α-Tricalcium Phosphate 와 혈소판농축혈장의조합처리가 골모세포의 활성에 미치는 영향

Abstract

Platelet-rich plasma(PRP) has been used alone or in association with autograft, allograft and bone substitutes to promote the bone healing and the new bone formation in implant dentistry, Q-Tcr is bone substitute which is suited to bone regeneration, PRP contain several growth factors which stimulate osteogenesis in early stage of bone healing, and thrombin is compounds that expedite the progress of blαld coagulation for gelation of PRP. The purpose of this study was to investigate the effects of Q-TCP, PRP and thrombin 011 cell viability, cell adhesion activity or ALPase activity of human fetal osteoblast-l(HFOB-1l. HFOB-l was treated with Q-TCP, PRP and thrombin only or combination. After the HFOB-l was cultured in the same condition for 48 hrs, absorbance was measured for cell viability by MTT assay, for cell adhesion activity by XTr assay and for ALPase activity by ALPase ässay. The light microscopic study was c빼ed out to observe morphological changes of cultured HFOB-1. The results were as follows : 1. a-TCP increased cell viability and cell adhesion activity of HFOB• 1 to 엉 )lM concel1tration, but a-TCP decreased them above the concentration of 25 )lM 2. PRP was increased cell viability and cell adhesion activity of ]-IFOB-l to 50 μe!mC concentration, but PRP decreased them above the concentration of 100 )lM. 3. In Q-TCr(l ~ 100 !1M) and rRP(12 μe!뼈) treated groups, they statistic significantly increased cell viability, cell adhesion activity and ALPase activity(P<O.Ol) lo 50 )lM Q-TCP, but the combinatiol1 groups decreased them above the 100 !1M concentration of Q-TCP 4. In Q-TCP(l ~ 100 !1M), PRP 12 μe!mQ and thrombin 4 !1M trmted groups, they statistically significant increased cell viability, cell adhesion activity and ALrase activity, but the combination groups decreased them above the 50 !1M concentration of Q-TCP These results suggest that the growth of HFOB-l is very effective in the combination of 50 11M Q-TCP and 12 띠/l1l e PRP. While, the growth effect was decreased in the combination of 50 !1M Q-TCP 8nd 12 띠/me PRP to 4 11M thrombin The combination of concentration among Q-TCP, PRP, and thrombin is very important in osteoblastic growth.