Antioxidant and anti-wrinkle effects of Ixeridium dentatum (Thunb.) Tzvelev ethanol extract
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초록
This study aimed to confirm the potential of 70% ethanol extract of Ixeridium dentatum (70EID) as an antioxidant and anti-wrinkle cosmetic material. DPPH radical scavenging assay and ABTS+ radical scavenging assay were performed to evaluate antioxidant activity, and elastase inhibition assay and collagenase inhibition assay were performed to evaluate anti-wrinkle activity. In addition, the cytotoxicity of 70EID was evaluated using CCD-986sk fibroblasts, and the inhibition of UV-induced MMP-1 expression was confirmed using the UVB-irradiated CCD-986sk fibroblast model. DPPH and ABTS+ radical scavenging activity were 72% and 93% at 1000 μg/mL 70EID, respectively. In addition, the elastase and collagenase inhibition rates were 38% and 61% at 1000 μg/mL 70EID, respectively. The survival rates of CCD-986sk fibroblast by 70EID were 100%, 93%, and 88% at 25, 50, and 100 μg/mL, respectively. The final concentrations were set at 25 and 50 μg/mL, at which more than 90% of cells survived. 50 μg/mL 70EID significantly reduced UVB-induced protein and mRNA expression of MMP-1. Based on these results, I suggest that 70EID has a high potential for use as an antioxidant and anti-wrinkle cosmetic material.
Abstract
This study aimed to confirm the potential of 70% ethanol extract of Ixeridium dentatum (70EID) as an antioxidant and anti-wrinkle cosmetic material. DPPH radical scavenging assay and ABTS+ radical scavenging assay were performed to evaluate antioxidant activity, and elastase inhibition assay and collagenase inhibition assay were performed to evaluate anti-wrinkle activity. In addition, the cytotoxicity of 70EID was evaluated using CCD-986sk fibroblasts, and the inhibition of UV-induced MMP-1 expression was confirmed using the UVB-irradiated CCD-986sk fibroblast model. DPPH and ABTS+ radical scavenging activity were 72% and 93% at 1000 μg/mL 70EID, respectively. In addition, the elastase and collagenase inhibition rates were 38% and 61% at 1000 μg/mL 70EID, respectively. The survival rates of CCD-986sk fibroblast by 70EID were 100%, 93%, and 88% at 25, 50, and 100 μg/mL, respectively. The final concentrations were set at 25 and 50 μg/mL, at which more than 90% of cells survived. 50 μg/mL 70EID significantly reduced UVB-induced protein and mRNA expression of MMP-1. Based on these results, I suggest that 70EID has a high potential for use as an antioxidant and anti-wrinkle cosmetic material.
참고문헌 (22)
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