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증숙 아피오스(Apios americana Medikus) 에탄올 추출물의 항산화, 항염증 및 안자극 평가

Antioxidant, Anti-inflammatory and eye irritation evaluation of Steamed Apiose(Apios americana Medikus) Ethanol Extract

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초록

This study was conducted to confirm the use possibility as a new natural functional material through antioxidant and anti-inflammatory studies using manufacturing Steamed apios ethanol extract (SAEE) which is made by same as red ginseng process. First, to compare the antioxidant activity of apios ethanol extract (AEE) and SAEE, total polyphenol content, ABTS+ radical scavenging ability, and DPPH electron donating ability were measured. To confirm the anti-inflammatory effect, NO production, and COX-2 and iNOS protein expression were measured in RAW 264.7 cells. To confirm the safety, short term exposure (STE) test and bovine corneal opacity and permeability (BCOP) assay which are alternative eye irritation tests, were conducted. By putting the results of this study together, the antioxidant activity, anti-inflammatory effect, and stability of SAEE were confirmed, so that steamed apiose can be expected to be practical as a new functional natural extract in the future.

Abstract

This study was conducted to confirm the use possibility as a new natural functional material through antioxidant and anti-inflammatory studies using manufacturing Steamed apios ethanol extract (SAEE) which is made by same as red ginseng process. First, to compare the antioxidant activity of apios ethanol extract (AEE) and SAEE, total polyphenol content, ABTS+ radical scavenging ability, and DPPH electron donating ability were measured. To confirm the anti-inflammatory effect, NO production, and COX-2 and iNOS protein expression were measured in RAW 264.7 cells. To confirm the safety, short term exposure (STE) test and bovine corneal opacity and permeability (BCOP) assay which are alternative eye irritation tests, were conducted. By putting the results of this study together, the antioxidant activity, anti-inflammatory effect, and stability of SAEE were confirmed, so that steamed apiose can be expected to be practical as a new functional natural extract in the future.

참고문헌 (39)

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