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트레드밀 운동과 MitoQ 처치가 D-galactose로 유도된 노화 쥐의 혈관기능에 미치는 영향

Effects of treadmill exercise and MitoQ treatment on vascular function in D-galactose-induced aging rat

황동주|최동훈|구정훈|조준용|오유성|김동원|권기천
국민체육진흥공단 한국스포츠과학원 (Korea Institute of Sport Science)|2019.12|체육과학연구 (Korean Journal of Sport Science)|제30권 제4호|pp. 689-699|DOI 10.24985/kjss.2019.30.4.689

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참고문헌

키워드

노화Aging혈관내피세포활성산소Endothelial cellReactive Oxygen Species트레드밀 운동혈관기능vascular function마이토큐Treadmill ExerciseMitoQ
주제분류체육

초록

[목적] 본 연구의 목적은 D-galactose로 유도된 노화 쥐를 대상으로 트레드밀 운동과 MitoQ 처치가 대동맥 내 NADPH oxidase와 항산화 및 혈관기능에 미치는 영향을 알아보는데 있다. [방법] 노화 유도는 D-galactose를 생리식염수에 녹여 100 mg/kg의 용량을 총 10주간 1일 1회 SD rat(Sprague-Dawley rat) 복강 내 주사(intraperitoneal injection)하였다. 실험집단은 Control group(CON, n=9), D-galactose control group(DC, n=9), D-galactose+MitoQ group(DM, n=9), D-galactose+ Exercise group(DE, n=9), D-galactose+MitoQ+Exercise group(DME, n=9)의 총 5개의 집단으로 구분하였으며, 트레드밀 운동은 주 5일 8주간 점진적으로 증가시키는 운동 강도로 실시하였고 MitoQ 처치는 운동이 실행되는 8주 동안 주 2회 100μM/kg의 농도로 희석하여 복강 내 주사하였다. [결과] 트레드밀 운동과 MitoQ 처치는 NADPH oxidase의 수준을 감소시키고 SOD-2, catalase와 같은 항산화효소를 증가시켜 혈관기능에 관여하는 단백질인 eNOS의 수준을 증가시키는 반면 VCAM-1의 수준은 감소시키는 긍정적인 효과를 보였다. 이를 통한 혈관 내중막 두께를 감소시킬 뿐만 아니라 인지능력 또한 유의하게 증가시켰다. [결론] 따라서 운동과 MitoQ 처치는 노화로 인한 혈관기능장애를 개선하고 치료하는데 효과적인 것으로 나타났다.

Abstract

[Purpose] The purpose of this study was to investigate the effect of treadmill exercise and MitoQ treatment on NADPH oxidase, antioxidation and vascular function-related factors in aortic of D-galactose-induced aging rats. [Methods] To induce the animal model of aging, D-galactose was diluted in saline, and a dose of 100mg /kg was intraperitoneally injected into Sprague-Dawley rats once a day for a total of 10 weeks. Rats were divided into five groups: Control group (CON, n=9), D-galactose control group (DC, n=9), D-galactose+MitoQ group (DM, n=9), D-galactose+Exercise group (DE, n=9), D-galactose+MitoQ + Exercise group(DME, n=9), and treadmill exercise was conducted for 5 days/week during 8 weeks with gradual increase of intensity. MitoQ treatment was intraperitoneally injected at a concentration of 100μM/kg twice a week for 8 weeks during the research period. [Results] The result showed that treadmill exercise and MitoQ treatment decreased the expression of NADPH oxidase level and increased antioxidant enzyme such as SOD-2, catalase. It lead to positive effects such as increasing the level of eNOS, a protein involved in vascular function while decreasing the level of VCAM-1. In addition, as a result, it showed the structurally reduced intima-media thickness. [Conclusions] It can be concluded that treadmill exercise and MitoQ treatment are effective in ameliorating and treating vascular dysfunction resulting from aging.

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