운동과 홍삼섭취가 고지방식이 노화흰쥐의 혈관에서산화적 스트레스, eNOS에 미치는 영향
Effect of Exercise and Korean Red Ginseng on Aortic Oxidative Stress and eNOS in against Aging-induced Rats with High Fat Diet
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노화와 고지방식이에 따른 혈관은 산화적 스트레스와 관련된 인자들을 유발하며, 장기간 지속된 산화적 스트레스는 표적 장기의 세포들을 손상시킨다. 운동과 홍삼은 항산화효소들을 활성시키고, 산화적스트레스로부터 보호한다고 알려져 있다. 이에 이 연구에서는 고지방식이 노화흰쥐의 혈관에서 산화적스트레스 표지자, 항산화효소, Ang II, 그리고 eNOS 발현을 조사하고 운동과 홍삼섭취를 병행하여 그 차이에 따른 효과를 규명하는데 목적을 두고 조사하였다. 연구대상은 SD계 숫컷 흰쥐를 이용하여 D-galactose (D-gal)를 투여하여 노화를 유도하였고, 모두 6군으로 8마리씩 나누어 다음과 같이 분류하였다. 2개월령 청년그룹 (Y-C), 노화그룹(O-C), 노화고지방그룹(OHF), 노화고지방 운동그룹(OHF-Ex), 노화고지방 운동+홍삼그룹(OHF- Ex+ RG), 그리고 노화고지방 홍삼그룹(OHF-RG)이며, 노화유도는 D-gal (50mg/kg)로 매일 주입하여 총 9주간 시행하였고, 식이는 일반식이와 고지방식이로 하였다. 운동으로는 수영을 시행하였고, 방법은 주당 5회 60분씩 총 8주간 수행하였으며, 홍삼섭취(200mg/kg)는 8주 동안 경구 투여하였다. 혈관에서 산화적 스트레스 표지자인 MDA, TBARS, Ang II는 노화고지방식이그룹이 증가하였으며, 운동군, 홍삼군, 그리고 운동+홍삼군의 GPx, SOD, eNOS는 증가하였다. 그러므로 이상의 경과를 종합하면 운동과 홍삼섭취 그리고 복합처치는 고지방식이 노화혈관에서 산화적 스트레스와 Ang II를 억제함으로써 혈관손상 억제효과가 있을 것으로 생각된다.
Abstract
Aging vascular with high fat diet (HF) is associated with an increased risk of oxidative stress and long term oxidative stress causes cell damage in target organs. Exercise (Ex) and Korean red ginseng (RG) are known to activity anti-oxidants enzyme and protect from oxidative stress. Thus, the purpose of this study was to examine effect of Ex and RG on antioxidantive enzymes, Angiotensin II (Ang II), and endothelial nitric oxide synthase (eNOS) in aorta tissue of aging rats with HF for 8 weeks. The aging model rats induced by D-galatose (D-gal) using SD rats and divided into 6 groups: (1) Young control group (Y-C), (2) Old control group (O-C), (3) O-C with HF group (OHF), (4) OHF with Ex group (OHF-Ex), (5) O-Ex with RG (OHF-Ex+RG), OHF with RG (OHF-RG). The D-gal injection (50 mg/kg) was given to intraperitoneally and fed an HF. The swimming was performed for 60min/day and the RG supplementation was orally given 200mg/kg/day. The MDA, TBARS and Ang II were elevated in the OHF group. The GPx, SOD, eNOS were increased in OHF-Ex, OHF-Ex+RG, and OHF-RG groups. These results suggest that Ex, RG or combination has a beneficial effect in inhibiting oxidative stress and Ang II in aging rat aorta.
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