Antioxidant activity of silkworm powder treated with protease
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The antioxidant activity of silkworm powder treated by proteolytic enzyme was investigated. Total protein content of silkworm power was assayed using BCA, Bradford assays and SDSpolyacrylamide gel electrophoresis (PAGE) with alkaline protease treatment conditions including temperature and pH. The optimum condition of alkaline protease treatment for silkworm powder was found to be 60oC and pH 7. The alkaline protease treatment resulted in increased contents of free amino acids, total polyphenol and total flavonoid compared to control group. The silkworm hydrolysates showed excellent antioxidant activities in various in vitro models such as 2,2 diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, 2,2 – azino-bis(3-ethylbenzthiazoline-6)-sulfonic acid (ABTS) radical scavenging activity. These results provide useful information for using silkworm powder as an ingredient in functional foods and for exploiting alkaline protease treatment to improve the extractability and bioactivity of a raw material.
Abstract
The antioxidant activity of silkworm powder treated by proteolytic enzyme was investigated. Total protein content of silkworm power was assayed using BCA, Bradford assays and SDSpolyacrylamide gel electrophoresis (PAGE) with alkaline protease treatment conditions including temperature and pH. The optimum condition of alkaline protease treatment for silkworm powder was found to be 60oC and pH 7. The alkaline protease treatment resulted in increased contents of free amino acids, total polyphenol and total flavonoid compared to control group. The silkworm hydrolysates showed excellent antioxidant activities in various in vitro models such as 2,2 diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, 2,2 – azino-bis(3-ethylbenzthiazoline-6)-sulfonic acid (ABTS) radical scavenging activity. These results provide useful information for using silkworm powder as an ingredient in functional foods and for exploiting alkaline protease treatment to improve the extractability and bioactivity of a raw material.
참고문헌 (39)
- Lee WC, "The strategy for the development of bioresources utilizing sericultural products and insects", Int J Indust Entomol, 1, pp.95~102 (2000)
- Jo YY, "The possibility of silk protein to the chondrogenesis", J Seric Entomol Sci, 50, pp.15~19 (2012)
- Foss C, "Silk fibroin", hyaluronic acid 3D matrices for cartilage tissue engineering, 2013, pp.14(1), 38~47
- Kweon H, "Hydroxyapatite and silk combination-coated dental implants result in superior bone formation in the peri-implant area compared with hydroxyapatite and collagen combination-coated implants", J Oral Maxillofac Surg, 72, pp.1928~1936 (2014)
- 주완택, "Effects of Physiological Active Substance Extracted from Silkworm Fece", International Journal of Industrial Entomology, 29(2), pp.179~184 (2014), 한국잠사학회
- 김용순, "Effect of Silkworm (Bombyx mori) Excrement Powder on the Alcoholic Hepatotoxicity in Rats", 생명과학회지, 18(10), pp.1342~1347 (2008), 한국생명과학회
- 류강선, "Effects of silkworm pupae on bone mineral density in ovariectomized rat model of osteoporesis", International Journal of Industrial Entomology, 24(2), pp.63~68 (2012), 한국잠사학회
- 전정우, "Effect of Silk Fibroin Hydrolysate on the Apoptosis of MCF-7 human Breast Cancer Cells", International Journal of Industrial Entomology, 27(2), pp.228~236 (2013), 한국잠사학회
- 주완택, "Quantitative Analysis of 1-Deoxynojirimycin Content Using Silkworm Genetic Resources", International Journal of Industrial Entomology, 29(2), pp.162~168 (2014), 한국잠사학회
- 주완택, "Quantitative analysis of rutin content using silkworm genetic resources", International Journal of Industrial Entomology, 31(2), pp.56~61 (2015), 한국잠사학회
- 김영호, "누에 분말 첨가빵이 Rat의 콜레스테롤 및 중성지질에 미치는 영향", 한국식품영양학회지, 21(3), pp.306~311 (2008), 한국식품영양학회
- Choi JH, "Effect of silkworm powder on oxygen radicals and their scavenger enzymes in brain membranes of SD rats", Korean J Seric Sci, 42, pp.93~98 (2000)
- Singhal P, "Studies on production, characterization and applications of microbial alkaline proteases", Int J Adv Biotecnol Res, 3, pp.653~669 (2012)
- Mabrouk SS, "Optimization of alkaline protease productivity by Bacillus licheniformis ATCC 21415", Bioresour. Technol, 69, pp.155~159 (1999)
- Mehrotra S, "The production of alkaline protease by a Bacillus species isolate", Bioresour Technol, 67, pp.201~203 (1999)
- Bradford MM., "A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of proteindye binding", Anal. Biochem, 72, pp.248~254 (1976)
- Smith PK, "Measurement of protein using bicinchoninic acid. Anal", Anal. Biochem, 150(1), pp.76~85 (1985)
- Laemmli UK, "Cleavage of structural proteins during the assembly of the head of bacteriophage T4", Nature, 227, pp.680~685 (1970)
- Singleton V, "Colorimetry of total phenolics with phosphomolibdic-phosphotungstic acid reagents", Am J Enol Vitic, 16, pp.144~158 (1965)
- Zhishen J, "The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals", Food Chem 64, 64, pp.555~559 (1999)
- Li X, "Composition of amino acids in feed ingredients for animal diets", Amino Acids, 40, pp.1159~1168 (2011)
- Hatano T, "Two new flavonoids and other constituents in licorice root: their relative astringency and radical scavenging effects", Chem Pharm Bull, 36(6), pp.2090~2097 (1988)
- Re R, "Antioxidant activity applying an improved ABTS radical cation decolorization assay", Free Radic Biol Med, 26, pp.1231~1237 (1999)
- McElderry LA, "Six methods for urinary protein compared", Clin Chem, 28(2), pp.356~360 (1982)
- Chutipongtanate S, "Systematic comparisons of various spectrophotometric and colorimetric methods to measure concentrations of protein, peptide and amino acid: detectable limits, linear dynamic ranges, interferences, practicality and unit costs", Talanta, 98, pp.123~129 (2012)
- Hernández-Ledesma B, "Preparation of antioxidant enzymatic hydrolysates from -lactalbumin and -lactoglobulin. Identification of active peptides by HPLC-MS", MS, 2005, pp.53, 588~593
- Udenigwe C, "Chemometric analysis of the amino acid requirements of antioxidant food protein hydrolysates", International Journal of Molecular Sciences, 12(5), pp.3148~3161 (2011)
- Ness AR, "Fruit and vegetables, and cardiovascular disease: a review", Int. J. Epidemiol, 26, pp.1~13 (1997)
- Halliwell B, "Antioxidant defense mechanisms: from the beginning to the end", Free Radic. Res, 31, pp.261~272 (1999)
- Adler-Nissen J, "Enzymic hydrolysis of food proteins" (1986), Elsevier Applied Science Publishers
- Liu Q, "Antioxidant activity and functional properties of porcine plasma protein hydrolysate as influenced by the degree of hydrolysis", Food Chem, 118, pp.403~410 (2010)
- Miller NJ, "Spectrophotometric determination of antioxidant activity", Redox Report, 2(3), pp.161~171 (1996)
- Sánchez-Moreno C, "A procedure to measure the antiradical efficiency of polyphenols", J Sci Food Agric, 76(2), pp.270~276 (1998)
- Cano A, "A method to measure antioxidant activity in organic media: application to lipophilic vitamins", Redox Report, 5, pp.365~370 (2000)
- Sharma B, "Antioxidant aproperties, physico-chemical characteristics and proximate composition of five wild fruits of Manipur, India", J Food Sci Technol, 52(2), pp.894~902 (2015)
- Wang T, "Enzyme-enhanced extraction of antioxidant ingredients from red algae Palmaria palmate", LWTFood Sci Technol, 43, pp.1387~1393 (2010)
- Shin DH, "Antioxidation substances in mulberry leaf", J Korean Oil Chem Soc, 16, pp.27~31 (1998)
- Kodama T, "Glucosylation of quercetin by a cell suspension culture of vitis species", Agric Biol Chem, 54, pp.3238~3288 (1990)
- 채주영, "뽕잎 품종별 기능성 성분 분석", 한국식품영양과학회지, 32(1), pp.15~21 (2003), 한국식품영양과학회