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LC-MS/MS를 이용한 하귤 (Citrus natsudaidai Hayata) 껍질의 플라보노이드 성분분석: 항산화 활성에 대한 기여

Determination of Flavonoids in the Peel of Citrus Fruit (Citrus natsudaidai Hayata) Using a HPLC-MS/MS: Contribution to the Overall Antioxidant Activity

박세민|조영아|김재훈|이지은|송이|정성우|신성철|이원섭|김곤섭|허무룡|김해경|옥세윤
농업생명과학연구원 (Institute of Agriculture & Life Science, Gyeongsang National University)|2013|농업생명과학연구 (Journal of Agriculture & Life Science)|제47권 제4호|pp. 39-49

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참고문헌

키워드

플라보노이드Antioxidant activity항산화활성FlavonoidsLiquid chromatography-tandem mass spectrometry하귤고성능 액체크로마토그래피-이중질량분석법폴리메톡시플라본Citrus natsudaidaiPolymethoxyflavones

초록

감귤류의 껍질은 플라보노이드의 중요한 소스 중의 하나로서 동아시아에서 내장 및 염증성 질환을 치료하는 민간 의약품으로서 사용되어 왔다. 본 연구에서는 하귤 (C. natsudaidai) 껍질에 포함되어 있는 플라보노이드 성분을 고성능액체크로마토그래피-이중질량분석법 (HPLC-MS/MS)으로 10 개 성분을 동정하였다. 플라바논, 플라본 및 쿠마린 유도체는 각각 hesperetin, noviletin 및 coumarin을 사용하여 유효화 하였으며 유효화된 방법으로 정량하였다. 상관계수 (r2)는 >0.9970으로서 높은 값을 보여주었다. LOD는 >0.01 mg/L이었으며 LOQ는 >0.05 mg/L이었다. 플라보노이드의 총량은 9229.7 ± 0.5 mg/kg 이었다. Naringin의 량이 5010.0 ± 4.5 mg/kg으로 가장 많았으며 sinensetin의 량이 0.6 ± 0.1 mg/kg으로 가장 적었다. 항산화력을 25 μg/mL 에서 500 μg/mL의 농도범위에서 DPPH·, ABTS·+, NO· 소거능 및 FRAP의 항으로 분석하였다. 감귤 플라보노이드의 항산화 능력은 시료의 량이 증가하면 증가함을 알 수 있다.

Abstract

The fruit peel of Citruses is an important flavonoid source and used as folk medicines for curing intestine and inflammatory diseases. In this work, 9 flavonoids and one coumarin derivatives in Hagyul (Citrus natsudaidai Hayata) peel were characterized using high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Flavanones, flavones and coumarin derivative were validated in terms of hesperetin, noviletin and coumarin, respectively, and quantified using the validated methods. Correlation coefficients (r2) were >0.9970, indicating accetable. LOD's and LOQ's were above 0.01 and 0.05 mg/L, respectively. Total amount of the flavonoids were 플9229.7 ± 0.5 mg/kg. The concentration of naringin was highest as 5010.0 ± 4.5 mg/kg and sinensetin was lowest as 0.6 ± 0.1 mg/kg. Antioxidant capacities were evaluated between 25 and 500 μg/mL in terms of DPPH·, ABTS·+ NO· scavenging and FRAP. The antioxidant acitvities of the flavonoids increased dose-dependantly.

참고문헌 (37)

  1. Anagnostopoulou, M. A., "Radical scavenging activity of various extracts and fractions of sweet orange peel (Citrus sinensis)", Food Chem, 94, pp.19~25 (2006)
  2. Barros, L., "Total phenols, ascorbic acid, β-carotene and lycopene in Portuguese wild edible mushrooms and their antioxidant activities", Food chem, 103, pp.413~419 (2007)
  3. Benzie, I. F, "The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay", Anal. Biochem, 239, pp.239~270 (1996)
  4. Blois, M. S., "Antioxidant determinations by the use of a stable free radical", Nature, 181, pp.1199~1200 (1958)
  5. Bok, S. H., "Plasma and Hepatic Cholesterol and Hepatic Activities of 3-Hydroxy-3-methyl-glutaryl-CoA Reductase and Acyl CoA: Cholesterol Transferase Are Lower in Rats Fed Citrus Peel Extract or a Mixture of Citrus Bioflavonoids", J. Nutr, 129, pp.1182~1185 (1999)
  6. Bozin, B., "Phenolics as antioxidants in garlic (Allium sativum L., Alliaceae)", Food Chem, 111, pp.925~929 (2008)
  7. Chung, S. K., "Status of Citrus fruit production and view of utilization in Cheju", Food Ind. Nutri, 5, pp.42~52 (2000)
  8. Dandan, W., "Identification of polymethoxylated flavones from green tangerine peel (Pericarpium Citri Reticulatae Viride) by chromatographic and spectroscopic techniques", J. Pharm. Biomed. Anal, 44, pp.63~69 (2007)
  9. Domon, B., "A systematic nomenclature for carbohydrate fragmentations in FAB-MS", MS spectra of glycoconjugates, 1988, pp.5, 397~409
  10. Dugo, P., "LC-MS for the identification of oxygen heterocyclic compounds in citrus essential oils", J. Pharm. Biomed. Anal, 24, pp.147~154 (2000)
  11. El-Shafae, A. M., "Bioactive polymethoxyflavones and flavanone glycosides from the peels of Citrus deliciosa", Zhonghua yao xue za zhi, 54, pp.199~206 (2002)
  12. Gutfinger, T., "Polyphenols in olive oils", J. Am. Oil Chem. Soc, 58, pp.966~968 (1981)
  13. Han, S. S., "Studies on antimicrobial activities and safety of natural naringin in Korea", Kor. J. Mycol, 16, pp.33~40 (1988)
  14. Hirano, T., "Citrus flavone tangeretin inhibits leukaemic HL-60 cell growth partially through induction of apoptosis with less cytotoxicity on normal lymphocytes", Br. J. Cancer, 72, pp.1380~1388 (1995)
  15. Iio, M., "Flavonoids as a Possible Preventive of Dental Caries", Agric. Biol. Chem, 48, pp.2143~2145 (1984)
  16. Jeong, W. S., "The Antioxidative Activity of Korean Citrus unshiu Peels", Food Sci. Biotechnol, 6, pp.292~296 (1997)
  17. Kanes, K., "Phenolic composition of various tissues of rutaceae species", Phytochemistry, 32, pp.967~974 (1993)
  18. Kim, B. K., "Effects of Citrus Flavonoid, Hesperidin and Naringin on Lipid Metabolism in HepG2 Cells", Korean J. Life Sci, 9, pp.382~388 (1999)
  19. Kim, H. G., "Flavonoid profiling in three citrus varieties native to the Republic of Korea using liquid chromatography coupled with tandem mass spectrometry:contribution to overall antioxidant activity", Biomed. Chromatogr, 26, pp.464~470 (2012)
  20. Kim, H. G., "Determination of the change of flavonoid components as the defence materials of Citrus unshiu Marc. fruit peel against Penicillium digitatum by liquid chromatography coupled with tandem mass spectrometry", Food Chem, 128, pp.49~54 (2011)
  21. Kim, H. J., "Effect of hesperidin extracted from tangerine peel on Cd and lipid metabolism, and antioxidative capacity in rats", Korean J. Nutr, 32, pp.137~149 (1999)
  22. Kurowska, E. M., "Hypocholesterolemic effects of dietary citrus juices in rabbits", Nutr. Res, 20, pp.121~129 (2000)
  23. Lee, J. H., "Characterisation of flavonoids in Orostachys japonicus A. Berger using HPLC–MS", MS: contribution to the overall antioxidant effect, 2011, pp.124, 1627~1633
  24. Li, X., "LC–MS", MS determination of naringin, hesperidin and neohesperidin in rat serum after orally administrating the decoction of Bulpleurum falcatum L. and Fractus aurantii, 2004, 159~166
  25. Ma, Y. L., "Characterization of flavone and flavonol aglycones by collision-induced dissociation tandem mass spectrometry", Commun. Mass Spectrometry, 11, pp.1357~1364 (1997)
  26. Machida, K, "On the Flavonoid constituents from the Peels of Citrus hassaku HORT. ex TANAKA", Chem. Pharm. Bull, 37, pp.1092~1094 (1989)
  27. Marcocci, L., "The nitric oxide scavenging properties of Ginko biloba extract EGB 761", Biochem. Biophys. Res. Commun, 201, pp.748~755 (1994)
  28. McLachlan, D. G., "Automated Gas Chromatographic Method for the Determination of Ethanol in Canned Salmon", J. Agric. Food Chem, 47, pp.217~220 (1999)
  29. Mizuno, M., "Chemotaxonomy of the Genus Citrus Based on Polymethoxyflavones", Chem. Pharm. Bull, 39, pp.945~949 (1991)
  30. Mokbel, M. S, "Evaluation of the antioxidant activity of extracts from buntan (Citrus grandis Osbeck) fruit tissues", Food Chem, 94, pp.529~534 (2006)
  31. Monforte, M. T., "Biological effects of hesperidin, a Citrus flavonoid. (note II): hypolipidemic activity on experimental hypercholesterolemia in rat", Farmaco, 50, pp.595~599 (1995)
  32. Re, R., "Antioxidant activity applying an improved ABTS radical cation decolorization assay", Free Radic. Biol Med, 26, pp.1231~1237 (1999)
  33. Shi, P., "Characterization and identification of isomeric flavonoid O-diglycosides from genus Citrus in negative electrospray ionization by ion trap mass spectrometry and time-of-flight mass spectrometry", Anal. Chim. Acta, 598, pp.110~118 (2007)
  34. Son, H. S., "Isolation, Purification and Hypotensive Effect of Bioflavonoids in Citrus sinensis", J. Korean Soc. Food Nutr, 21, pp.136~142 (1992)
  35. Hee Sun Song, "In Vitro Antioxidant Activity Profiles of β-Glucans Isolated from YeastSaccharomyces cerevisiae and Mutant Saccharomyces cerevisiae IS2", Food Science and Biotechnology, 15(3), pp.437~440 (2006), 한국식품과학회
  36. Xu, G. H., "Minerals, Phenolic Compounds, and Antioxidant Capacity of Citrus Peel Extract by Hot Water", Food Sci, 73, pp.C11~C18 (2008)
  37. Yuan, J. P., "Isomerization of trans-Astaxanthin to cis-Isomers in Organic Solvents", J. Agric. Food. Chem, 47, pp.3656~3660 (1999)

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