수확시기별 오렌지 5품종 부산물의 플라보노이드 함량과 항산화 활성
Flavonoids composition and antioxidant activity of by-products of five orange cultivars during maturation
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초록
This study was conducted to investigate total flavonoid content, flavonoid composition, and free radical scavenging effects of by-products from five orange cultivars during the period September 2015 to February 2016. Total flavonoid content was highest in peel extracts from immature fruit harvested in September. Total flavonoid contents of all cultivars mostly decreased while ripening. Among the five cultivars, total flavonoid content was highest in Hamlin sweet orange (21.66 mg/g), followed by Sanguinello blood orange (20.39 mg/g), Shamouti orange (18.49 mg/g), Tarocco blood orange (18.46 mg/g), and Olinda Valencia orange (17.07 mg/g). With regard to flavonoid composition, all cultivar materials had high levels of narirutin and hesperidin, but naringin and neohesperidin were not detected in any of the materials. Nobiletin, as polymethoxyflavone, was detected within a 23~40 μg/mL range in the immature fruit of all cultivars. Among all cultivars, antioxidant activities were higher in peel extracts than in pulp extracts. DPPH radical scavenging activities of peel extracts ranged from 40% to 58% while the ABTS radical scavenging activity range was 90~94%. The results indicate that orange, by-products, especially peel of immature orange fruit, could have application as natural materials with antioxidative effects.
Abstract
This study was conducted to investigate total flavonoid content, flavonoid composition, and free radical scavenging effects of by-products from five orange cultivars during the period September 2015 to February 2016. Total flavonoid content was highest in peel extracts from immature fruit harvested in September. Total flavonoid contents of all cultivars mostly decreased while ripening. Among the five cultivars, total flavonoid content was highest in Hamlin sweet orange (21.66 mg/g), followed by Sanguinello blood orange (20.39 mg/g), Shamouti orange (18.49 mg/g), Tarocco blood orange (18.46 mg/g), and Olinda Valencia orange (17.07 mg/g). With regard to flavonoid composition, all cultivar materials had high levels of narirutin and hesperidin, but naringin and neohesperidin were not detected in any of the materials. Nobiletin, as polymethoxyflavone, was detected within a 23~40 μg/mL range in the immature fruit of all cultivars. Among all cultivars, antioxidant activities were higher in peel extracts than in pulp extracts. DPPH radical scavenging activities of peel extracts ranged from 40% to 58% while the ABTS radical scavenging activity range was 90~94%. The results indicate that orange, by-products, especially peel of immature orange fruit, could have application as natural materials with antioxidative effects.
참고문헌 (26)
- Senevirathne M, "Effective drying of citrus by-product by high speed drying: A novel drying technique and their antioxidant activity", J Food Eng, 92, pp.157~163 (2009)
- Benelli P, "Bioactive extracts of orange (Citrus sinensis L. Osbeck) pomace obtained by SFE and low pressure techniques: Mathematical modeling and extract composition", J Supercrit Fluids, 55, pp.132~141 (2010)
- 김용석, "천연 휘발성 항균물질의 연구현황과 식품가공에의 이용", 한국식품과학회지, 35(2), pp.159~165 (2003), 한국식품과학회
- Bravo L, "Chemistry, dietary sources, metabolism, and nutritional significance", Nutr Rev, 56, pp.317~333 (1998)
- Cha JY, "Effects of hesperetin and naringenin on lipid concentration in orotic acid treated mice", Korean J Life Sci, 9, pp.389~394 (1999)
- Kamiya S, "Recent advances in the chemistry of the citrus flavonoids", Nippn Shokuhin Kogyo Gakkaishi, 18, pp.38~48 (1971)
- Moresi M, "Production of biomass untreated orange peel by Fusarium avenaceum", Appl Microbiol Biotechnol, 27, pp.37~45 (1987)
- Sohn JS, "Effect of hesperidin and naringin on antioxidative capacity in the rat", Korean J Nutr, 31, pp.687~696 (1998)
- Yoshigai E, "Citrus nobiletin suppresses inducible nitric oxide synthase gene expression in interleukin-1β-treated hepatocytes", Biochem Biophys Res Commun, 439, pp.54~59 (2013)
- Moreno MIN, "Comparison of the free radical-scavenging activity of propolis from several regions of Argentina", J Ethnopharmacol, 71, pp.109~114 (2000)
- Park HS, "Minor effects of the Citrus flavonoids naringin, naringenin and quercetin, on the pharmacokinetics of doxorubicin in rats", Pharmazie, 66, pp.424~429 (2011)
- Sasaki K, "Cloning and characterization of naringenin 8-prenyltransferase, a flavonoid-specific prenyltransferase of Sophora flavescens", Plant Physiol, 146, pp.1075~1084 (2008)
- Zhang J, "Isolation and identification of antioxidant compounds in Vaccinium bracteatum Thunb. by UHPLC-Q-TOF LC", MS and their kidney damage protection, 2014, pp.11, 62~70
- Blois MS, "Antioxidant determination by the use of a stable free radical", Nature, 181, pp.1199~1200 (1958)
- Re R, "Antioxidant activity applying an improved ABTS radical cation decolorization assay", Free Radic Biol Med, 26, pp.1231~1237 (1999)
- Heim KE, "Flavonoid antioxidants: Chemistry, metabolism, and structure- activity relationships", J Nutr Biochem, 13, pp.572~584 (2002)
- Jia Z, "The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals", Food Chem, 64, pp.555~559 (1999)
- 김은진, "자생식물과 생약자원 추출물의 폴리페놀, 플라보노이드 함량 및 항산화 활성 탐색", 한국식품과학회지, 44(3), pp.337~342 (2012), 한국식품과학회[DOI]
- Diaz-Mulab HM, "Changes in hydrophilic and lipophilic antioxidant activity and related bioactive compounds during postharvest storage of yellow and purple plum cultivars", Postharvest Biol Technol, 51, pp.354~363 (2009)
- Kibola J, "Phytochemicals and antioxidant activity of different fruit fractions(peel, pulp, aril, and seed)of Thai gac(Momordica cochinchinensis Spreng)", Food Chem, 127, pp.1138~1145 (2011)
- 이봉한, "2008년 국민건강영양조사에 근거한 과채류 주스 음용으로부터 한국인의 일인당 하루 총페놀, 총플라보노이드 및 항산화능 섭취량 추정", 한국식품과학회지, 43(4), pp.475~482 (2011), 한국식품과학회
- Choi SY, "Correlation between flavonoid content and the NO production inhibitory activity of peel extracts from various citrus fruits", Biol Pharm Bull, 30, pp.772~778 (2007)
- Cha JY, "Biofunctional activities of citrus flavonoids", J Korean Soc Agric Chem Biotechnol, 44, pp.122~128 (2001)
- Tripoli E, "Citrus flavonoids: Molecular structure, biological activity and nutritional properties: A review", Food Chem, 104, pp.466~479 (2007)
- Katsube T, "Screening for antioxidant activity in edible plant products: comparison of low-density lipoprotein oxidation assay, DPPH radical scavenging assay, and Folin-Ciocalteu assay", J Agric Food Chem, 52, pp.2391~2396 (2004)
- 이민영, "과일 껍질의 비타민 C , 폴리페놀, 플라보노이드 함량과 항산화 활성", 한국식품과학회지, 44(5), pp.540~544 (2012), 한국식품과학회[DOI]