HPLC-MS/MS를 이용한 진귤(Citrus sunki Hort. ex Tanaka) 과피의 플라보노이드 성분 분석 및 항산화 활성
Determination of Flavonoids in the Peel of Jingyul (Citrus sunki Hort. ex Tanaka) using a HPLC-MS/MS and the Antioxidant Activity
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참고문헌
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초록
감귤류 과피는 플라보노이드의 중요 급원 중의 하나로 아시아에서 민간 의약품으로서 오래전부터사용되어 왔다. 본 연구에서는 진귤(Citrus sunki Hort. ex Tanaka) 과피에서 HPLC-MS/MS를 이용하여 9종의 플라보노이드 성분을 동정하였으며, hydroxypentamethoxyflavone과 dihydroxytetramethoxyflavone은진귤에서 처음으로 확인되었다. 총 플라보노이드의 항산화 활성은 25~500 mg/L의 농도범위에서 DPPH·, ABTS·+, NO· 소거능 및 환원력에 의해 측정되었으며, 시료의 첨가량이 많아짐에 따라 항산화 활성은 유의적으로 증가하였다.
Abstract
The peel of citruses is an important flavonoid source and used as folk medicines. In this work, 9flavonoids in Jingyul (Citrus sunki Hort. ex Tanaka) peel were characterized using high performanceliquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Hydroxypentamethoxyflavone anddihydroxytetramethoxyflavone were identified in the Jingyul for the first time. Antioxidant capacitieswere evaluated in the concentration range of 25~500 mg/L in terms of DPPH·, ABTS·+, NO·scavenging and reducing power by FRAP. The antioxidant activities of total flavonoids increaseddose-dependantly.
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