주목 식물세포(Taxus chinensis) 배양 유래 Paclitaxel 정제를 위한 분별침전에서 제타전위 영향
Effect of Zeta Potential on Fractional Precipitation for the Purification of Paclitaxel from Plant Cell Cultures of Taxus chinensis
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초록
This study evaluated the effect of the zeta potential of silica-alumina on the behavior, in terms of purity, yield, fractional precipitation time, precipitate shape, size of fractional precipitation in the increased surface area, and the fractional precipitation process, for the purification of paclitaxel. As the zeta potential value of silica-alumina increased, the yield of paclitaxel concurrently increased while the precipitation time decreased. The use of alumina with the highest value of the zeta potential (+35.41 mV) as a surface area-increasing material dramatically reduced the precipitation time by 12 h compared with the results of the control. On the other hand, the purity of paclitaxel had almost no effect on changes in the zeta potential of silicaalumina. In addition, the precipitate size was inversely correlated with the absolute value of the zeta potential.
Abstract
This study evaluated the effect of the zeta potential of silica-alumina on the behavior, in terms of purity, yield, fractional precipitation time, precipitate shape, size of fractional precipitation in the increased surface area, and the fractional precipitation process, for the purification of paclitaxel. As the zeta potential value of silica-alumina increased, the yield of paclitaxel concurrently increased while the precipitation time decreased. The use of alumina with the highest value of the zeta potential (+35.41 mV) as a surface area-increasing material dramatically reduced the precipitation time by 12 h compared with the results of the control. On the other hand, the purity of paclitaxel had almost no effect on changes in the zeta potential of silicaalumina. In addition, the precipitate size was inversely correlated with the absolute value of the zeta potential.
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