(+)-Dihydromyricetin 정제를 위한 분별침전공정 개선
Improvement of the Fractional Precipitation Process for the Purification of (+)-Dihydromyricetin
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다운로드
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참고문헌
키워드
초록
Fractional precipitation is a simple method for purifying (+)-dihydromyricetin extracted from biomass. However, the fractionalprecipitation process has been inherently problematic due to the lengthy precipitation time that is required. The fractional precipitationtime was shortened and (+)-dihydromyricetin yield was improved by increasing the surface area per working volume(S/V) of the reacting solution through the addition of a cation exchange resin (Amberlite 200, Amberlite IR 120Na, Amberlite IR120H, or Amberlite IRC 50). Most of the (+)-dihydromyricetin (>90%) could be obtained after about 16 h of fractional precipitationusing Amberlite 200. Since high-purity (+)-dihydromyricetin can be obtained at a high yield and the precipitation time canbe reduced by increasing the surface area available for precipitation, this improved method is expected to minimize solventusage and the size and complexity of the high performance liquid chromatography operation required for (+)-dihydromyricetinpurification.
Abstract
Fractional precipitation is a simple method for purifying (+)-dihydromyricetin extracted from biomass. However, the fractionalprecipitation process has been inherently problematic due to the lengthy precipitation time that is required. The fractional precipitationtime was shortened and (+)-dihydromyricetin yield was improved by increasing the surface area per working volume(S/V) of the reacting solution through the addition of a cation exchange resin (Amberlite 200, Amberlite IR 120Na, Amberlite IR120H, or Amberlite IRC 50). Most of the (+)-dihydromyricetin (>90%) could be obtained after about 16 h of fractional precipitationusing Amberlite 200. Since high-purity (+)-dihydromyricetin can be obtained at a high yield and the precipitation time canbe reduced by increasing the surface area available for precipitation, this improved method is expected to minimize solventusage and the size and complexity of the high performance liquid chromatography operation required for (+)-dihydromyricetinpurification.
참고문헌 (24)
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