표면적이 증가된 분별침전에서 친수성 고분자물질을 이용한 paclitaxel의 입자 크기 감소
Decrease in the Particle Size of Paclitaxel by Increased Surface Area Fractional Precipitation Process Using Hydrophilic Polymer
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초록
본 연구에서는 표면적이 증가된 분별침전 공정에 친수성 고분자물질을 첨가하여 식물세포 유래 항암물질 paclitaxel의 입자 크기를 감소시키고자 하였다. 표면적이 증가된 분별침전 공정에 고분자물질(PVA, HPMC 2910, PVP-K90)을 첨가할 경우 침전물의 입자 크기는 획기적으로 감소하였다. 첨가된 고분자물질 중 PVP-K90가 가장 효과적이었으며, PVPK90 농도 0.2%(w/v)로 첨가한 경우 고분자물질을 첨가하지않은 경우보다 4−5배 정도 가장 작은 입자 크기(<20 μm radius)의 침전물을 얻었다. 또한 분별침전을 통해 얻은 침전물의 입자 크기는 분별침전 용액의 제타전위 절대값에 반비례함을 알 수 있었다.
Abstract
In this study, we applied an increased surface area fractional precipitation with hydrophilic polymer for decreasing the particle size of the anticancer agent paclitaxel from plant cell cultures. The addition of polymer resulted in a considerable decrease in the size of the paclitaxel precipitate. Among the polymers (HPMC 2910, PVP-K90, PVA) used, PVP-K90 was the most effective for the inhibition of precipitate growth. When PVP-K90 (0.2%, w/v) was used, the paclitaxel particles were four to five times smaller, having less than a 20 μm radius, than those obtained in the absence of the polymer. In addition, the precipitate size was inversely correlated with the absolute value of zeta potential.
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