Backbone 1H, 15N and 13C Resonance Assignment and Secondary Structure Prediction of HP0062 (O24902_HELPY) from Helicobacter pylori
2
조회
0
다운로드
3
인용
0
참고문헌
키워드
초록
HP0062 is an 86 residue hypothetical protein from Helicobacter pylori strain 26695. HP0062 was identified ESAT-6/WXG100 superfamily protein based on structure and sequence alignment and also contains leucine zipper domain sequence. Here, we report the sequence-specific backbone resonance assignment of HP0062. About 97.7% of all 1HN, 15N, 13Cα, 13Cβ and 13C=O resonances were assigned unambiguously. We could predict the secondary structure of HP0062 by analyzing the deviation of the 13Cα and 13Cβ chemical shifts from their respective random coil values. Secondary structure prediction shows that HP0062 consist of two α-helices. This study is a prerequisite for determining the solution structure of HP0062 and can be used for the study on interaction between HP0062 and DNA and other Helicobacter pylori proteins.
Abstract
HP0062 is an 86 residue hypothetical protein from Helicobacter pylori strain 26695. HP0062 was identified ESAT-6/WXG100 superfamily protein based on structure and sequence alignment and also contains leucine zipper domain sequence. Here, we report the sequence-specific backbone resonance assignment of HP0062. About 97.7% of all 1HN, 15N, 13Cα, 13Cβ and 13C=O resonances were assigned unambiguously. We could predict the secondary structure of HP0062 by analyzing the deviation of the 13Cα and 13Cβ chemical shifts from their respective random coil values. Secondary structure prediction shows that HP0062 consist of two α-helices. This study is a prerequisite for determining the solution structure of HP0062 and can be used for the study on interaction between HP0062 and DNA and other Helicobacter pylori proteins.
참고문헌 (14)
- Rothenbacher D, , Microbes Infect, 5(8), pp.693~703 (2003)
- Tomb J. F, , Nature, 388(6642), pp.539~547 (1997)
- Suerbaum S, , Nat Rev Microbiol, 5(6), pp.441~452 (2007)
- 김원재, "Backbone 1H, 15N, and 13C Resonance Assignment and Secondary Structure Prediction of HP1298 from Helicobacter pylori", Journal of the Korean Magnetic Resonance Society, 12(2), pp.65~73 (2008), 한국자기공명학회
- Jang S.B, , J. Biochem., 146(4), pp.535~540 (2009)
- Pallen M.J. T, , Trends Microbiol, 10(5), pp.209~212 (2002)
- 홍윤훈, "Structural Characterization of Growth-Related Translationally Controlled Tumor Protein P23", Journal of the Korean Magnetic Resonance Society, 5(1), pp.46~55 (2001), 한국자기공명학회
- Delaglio F, , J Biomol NMR, 6(3), pp.277~293 (1995)
- Johnson B. A, , Methods Mol Biol, 278, pp.313~352 (2004)
- Wishart D. S, , J Biomol NMR., 4(2), pp.171~180 (1994)
- Cornilescu G, , J Biomol NMR., 13(3), pp.289~302 (1999)
- Metzler W. J, , Biochem., 32(50), pp.13818~13829 (1993)
- Wishart D. S, , J Biomol NMR, 5(1), pp.67~81 (1995)
- Spera S, , J. Am. Chem. Soc., 113(14), pp.5490~5492 (1991)