Biochemical and Structural Characterization of HP1423 (Y1423_HELPY) from Helicobacter pylori
2
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
HP1423 (Y1423_HELPY) is a conserved hypothetical protein from H. pylori strain 26695. However, Sequence Blast result indicates that HP1423 belongs to S4 (PF01479) superfamily. According to Pfam database, the S4 domain is a small domain consisting of 60-65 amino acid residues, that probably mediates binding to RNA. In this study, we report the sequence-specific backbone resonance assignment of HP1423, which has 84 amino acid residues. We could assign unambiguously about 88% of all 1HN, 15N, 13Cα, 13Cβ and 13C=O resonances. We could not detect the resonances from residues 15-20, and disappearance of these peaks seems to be related with the intermediate-conformational exchange. These assigned NMR peaks of HP1423 can be used for studying the role of protein dynamics in millisecond timescale, and Protein-RNA binding.
Abstract
HP1423 (Y1423_HELPY) is a conserved hypothetical protein from H. pylori strain 26695. However, Sequence Blast result indicates that HP1423 belongs to S4 (PF01479) superfamily. According to Pfam database, the S4 domain is a small domain consisting of 60-65 amino acid residues, that probably mediates binding to RNA. In this study, we report the sequence-specific backbone resonance assignment of HP1423, which has 84 amino acid residues. We could assign unambiguously about 88% of all 1HN, 15N, 13Cα, 13Cβ and 13C=O resonances. We could not detect the resonances from residues 15-20, and disappearance of these peaks seems to be related with the intermediate-conformational exchange. These assigned NMR peaks of HP1423 can be used for studying the role of protein dynamics in millisecond timescale, and Protein-RNA binding.
참고문헌 (15)
- T.L. Cover, , Adv. Intern. Med., 41, pp.85~117 (1996)
- Sun-Bok Jang, "Backbone 1H, 15N and 13C Resonance Assignment and Secondary Structure Prediction of HP0062 (O24902_HELPY) from Helicobacter pylori", Journal of the Korean Magnetic Resonance Society, 13(2), pp.117~125 (2009), 한국자기공명학회
- 김원재, "Backbone 1H, 15N, and 13C Resonance Assignment and Secondary Structure Prediction of HP1298 from Helicobacter pylori", Journal of the Korean Magnetic Resonance Society, 12(2), pp.65~73 (2008), 한국자기공명학회
- A.P. Carter, , Nature, 407, pp.340~348 (2000)
- A. Matte, "Acta Crystallogr. Sect. F :Struct", Biol. Cryst. Commun., 61, pp.350~354 (2005)
- A. Yaremchuk, , EMBO J., 21, pp.3829~3840 (2000)
- B.L. Staker, , EMBO J., 19, pp.749~757 (2000)
- L. Volpon, , J. Bacteriol., 185, pp.4204~4210 (2003)
- L. Aravind, , J. Mol. Evol., 48, pp.291~302 (1999)
- F. Delaglio, , J. Biomol. NMR, 6, pp.277~293 (1995)
- B.A. Johnson, , Methods Mol. Biol., 278, pp.313~352 (2004)
- D.S. Wishart, , J. Biomol. NMR, 4, pp.171~180 (1994)
- G. Cornilescu, , J. Biomol. NMR, 13, pp.289~302 (1999)
- B.L. Staker, , EMBO J., 19, pp.749~757 (2000)
- K. Henzler-Wildman, , Nature, 450, pp.964~972 (2007)