^(1)H, ^(15)N and ^(13)C resonance assignment and secondary structure prediction of ss-DNA binding protein 12RNP2 precursor, HP0827 from Helicobacter pylori
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HP0827 has two RNP motif which is a very common protein domain involved in recognition of a wide range of ssRNA/DNA.We acquired 3D NMR spectra of HP0827 which shows well dispersed and homogeneous signals which allows us to assign 98% of all ^(1)H_N, ^(15)N, ^(13)C_α, ^(13)C_β and ^(13)C=O resonances and 90% of all sidechain resonances. The sequence-specific backbone resonance assignment of HP0827 can be used to gain deeper insights into the nucleic acids binding specificity of HP0827 in the future study. Here, we report secondary structure prediction of HP0827 derived from NMR data. Additionally, ssRNA/DNA binding assay studies was also conducted. This study might provide a clue for exact function of HP0827 based on structure and sequence.
Abstract
HP0827 has two RNP motif which is a very common protein domain involved in recognition of a wide range of ssRNA/DNA.We acquired 3D NMR spectra of HP0827 which shows well dispersed and homogeneous signals which allows us to assign 98% of all ^(1)H_N, ^(15)N, ^(13)C_α, ^(13)C_β and ^(13)C=O resonances and 90% of all sidechain resonances. The sequence-specific backbone resonance assignment of HP0827 can be used to gain deeper insights into the nucleic acids binding specificity of HP0827 in the future study. Here, we report secondary structure prediction of HP0827 derived from NMR data. Additionally, ssRNA/DNA binding assay studies was also conducted. This study might provide a clue for exact function of HP0827 based on structure and sequence.
참고문헌 (14)
- A. De Luca, , Cancer Lett, 213, pp.1~ (2004)
- S. Suerbaum, , Nat Rev Microbiol, 5, pp.441~ (2007)
- S. Renfrey, , Nat Rev Drug Discov, 1, pp.175~ (2002)
- Sun-Bok Jang, "Backbone 1H, 15N and 13C Resonance Assignment and Secondary Structure Prediction of HP0062 (O24902_HELPY) from Helicobacter pylori", Journal of the Korean Magnetic Resonance Society, 13(2), pp.117~125 (2009), 한국자기공명학회
- J. Dinq, , Genes Dev, 13, pp.1102~ (1999)
- F. Delaglio, , J Biomol NMR, 6, pp.277~ (1995)
- B. A. Johnson, , Methods Mol Biol, 278, pp.313~ (2004)
- T. D. Glddard, "SPARKY 3", University of California
- D. S. Wishart, , J Biomol NMR, 4, pp.171~ (1994)
- G. Cornilescu, , J Biomol NMR, 13, pp.289~ (1999)
- C. Maris, , FEBS Journal, 272, pp.2118~ (2005)
- L. Szilagyi, , Prog Nucl Magn Reson Spectrosc, 27, pp.325~ (1995)
- C. Antoine, , Nat Struct Mol Biol, 18, pp.443~ (2011)
- M. H. Lance, , Nature Protocols, 2, pp.1849~ (2007)