Backbone 1H, 15N, and 13C resonance assignments and secondary structure prediction of NifU-like protein, HP1492 from Helicobacter Pylori
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HP1492 is a NifU-like protein of Helicobacter pylori (H. pylori) and plays a role as a scaffold which transfer Fe-S cluster to Fe-S proteins like Ferredoxin. To understand how to bind to iron ion or iron-sulfur cluster, HP1492 was expressed and purified in Escherichia coli (E. coli). From the NMR measurement, we could carry out the sequence specific backbone resonance assignment of HP1492. Approximately 91% of all resonances could be assigned unambiguously. By analyzing results of CSI and TALOS from NMR data, we could predict the secondary structure of HP1492, which consists of three a-helices and three b-sheets. This study is an essential step towards the structural characterization of HP1492.
Abstract
HP1492 is a NifU-like protein of Helicobacter pylori (H. pylori) and plays a role as a scaffold which transfer Fe-S cluster to Fe-S proteins like Ferredoxin. To understand how to bind to iron ion or iron-sulfur cluster, HP1492 was expressed and purified in Escherichia coli (E. coli). From the NMR measurement, we could carry out the sequence specific backbone resonance assignment of HP1492. Approximately 91% of all resonances could be assigned unambiguously. By analyzing results of CSI and TALOS from NMR data, we could predict the secondary structure of HP1492, which consists of three a-helices and three b-sheets. This study is an essential step towards the structural characterization of HP1492.
참고문헌 (21)
- Blaser, M. J, , J Infect Dis, 161, pp.626~633 (1990)
- Forman, D, , BMJ, 302, pp.1302~1305 (1991)
- Parsonnet, J, , N Engl J Med, 330, pp.1267~1271 (1994)
- Smoot, D. T, , Gastroenterology, 113, pp.S31~50 (1997)
- Malfertheiner, P, , Gut, 61, pp.646~664 (2012)
- Tepes, B, , Helicobacter, 17(Suppl 1), pp.36~42 (2012)
- Moayyedi, P, , Am J Gastroenterol, 104, pp.3081~3083 (2009)
- Lill, R, , Nature, 460, pp.831~838 (2009)
- Yabe, T, , Plant Cell, 16 (2004)
- Rouault, T. A, , Trends Genet, 24 (2008)
- Fontecave, M, , Arch Biochem Biophys, 474, pp.226~237 (2008)
- Ayala-Castro, C, , Microbiol Mol Biol Rev, 72, pp.110~125 (2008)
- Schaible, U. E, , Nat Rev Microbiol, 2, pp.946~953 (2004)
- Reid, D. G, , J. Methods Mol Biol, 60, pp.1~28 (1997)
- Wishart, D. S, , J Biomol NMR, 6, pp.135~140 (1995)
- Delaglio, F, , J Biomol NMR, 6, pp.277~293 (1995)
- Johnson, B. A, , J Biomol NMR, 4, pp.603~614 (1994)
- Wuthrich, K, , pp.176~199 (1986), John Wiley & Sons
- Arseniev, A, , J Mol Biol, 201, pp.637~657 (1988)
- Gronenborn, A. M, , FEBS Lett, 243, pp.93~98 (1989)
- Wishart, D. S, , J Biomol NMR, 4, pp.171~180 (1994)