생쥐 작은창자 근육층의 calcitonin gene-related peptide 양성섬유의 분포 및 창자사이질세포에 대한 작용
Calcotonin gene-related peptide immunoreactivity in the muscle layer of small intestine; its action on interstitial cell
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초록
중추신경계 및 말초신경계에서 널리 분포하는 것으로 알려진 Calcitonin gene-related peptide (CGRP)는 소화관에서 창자신경계의 활성을 통하여, 소화관호르몬의 분비, 소화관운동의 조절, 근육층신경절의 흥분, 혈관의 확장 등에 관여하는 것으로 알려져 있다. 점막에서 역할은 근육층에 비해 잘 밝혀져 있지만, 근육층에서는 거의 감각신경전달물질로 작용하는 것으로 주로 알려지고 있다. 본 실험에서는 CGRP와 c-kit에 대한 항체를 이용하여 생쥐 작은창자근육층에서 면역조직화학적염색과 전기생리학적 방법을 이용하여 창자운동에 관한 CGRP의 작용을 알아보았다. 흰쥐 작은창자근육층에 존재하는 CGRP양성신경세포체와 신경돌기들이 창자근육층신경절에 널리 퍼 져있었고, 이중면역형광염색 결과 CGRP양성신경돌기들이 근육층신경얼기에서 창자사이질세포들과 밀접한 관련 이 있음을 밝혀내었다. 또한 전기생리학적으로 CGRP가 창자사이질세포의 전기생리학적 활성도를 억제한다는 것 도 알 수 있었다. 이러한 본 실험의 결과를 바탕으로 창자운동에 있어서 CGRP를 통한 창자사이질세포의 조절이 창자근육세포의 활성에 영향을 미친다고 추측된다.
Abstract
In addition to the central and the peripheral nervous system, calcitonin gene-related peptide-like immunoreactivity (CGRP-LI) has been identified throughout the enteric nervous system. Several functions of the CGRP in gastrointestinal (G-I) tract has been identified, but the effect of CGRP on G-I motility is unclear. The distribution of calcitonin gene-related peptide-like immunoreactivity (CGRP-LI) in the murine small bowel were studied by using immunohistochemistry, also analyzed functionally by using electrophysiological method. Immunohistochemical studies demonstrated that CGRP-LI is localized in both nerve fibers and myenteric ganglion cells in the whole-mount preparation of murine small intestine. Double labelling with CGRP and c-kit investigated by confocal microscope was shown that CGRP-LI enteric nerve fiber surrounded the c-kit positive interstitial cells of Cajal (ICC). Electrophysiological finding revealed that treatment of CGRP inhibited electrical activity on culture ICC. Our results suggest a CGRP innervation of murine small bowel ICC. The released CGRP from enteric nerve terminals may induce relaxation of small bowel through the inhibition of ICC.
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