Capsaicin이 성숙흰쥐 근육층신경얼기의 칼슘채널 α1B subunit 면역활성도에 미치는 영향
Changes of Immunoreactivities of Calcium Channel α1B Subunit in Myenteric Plexus of Capsaicin Treated Adult Rats
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성숙동물 근육층신경얼기에서 capsaicin 투여 후 시간경과에 따른 칼슘채널 α1B subunit의 면역활성도 변 화를 살펴보기 위해 면역조직화학적, 형태계측학적 방법을 이용해 비교분석 하였다. 성숙흰쥐(Sprague Dawley계, 200 gm 안팎)에 capsaicin을 50 mg/kg 피하주사하고 1일, 2일, 1주일, 4주일 후에 동 물을 희생시킨 후 돌창자 근육층신경얼기의 전층표본을 제작하여 NADH-TR 반응염색과 α1B subunit 면역조직화 학 염색을 시행하였다. 면역활성도를 정량화하기위해 신경세포의 형태를 분류하고 영상분석기를 이용하여 계수하 고 통계처리 하였다. 정상군의 근육층신경얼기에서 α1B subunit는 신경절과 신경절사이띠에 뚜렷한 면역반응을 나타내었으며 세포질 이 α1B subunit에 대해 반응을 나타낸 세포는 전체 신경세포의 56.0% (1형: 68.2%, 2형: 66.9%, 3형: 50.1%)를 차지 하고 있었다. α1B subunit에 대한 면역활성도는 capsaicin 투여 후 1일군부터 급격한 변화를 나타내어 면역반응 신 경세포의 총수가 19.0% 감소하였으며, 감소현상은 1형(20%↓)과 3형(40.6%↓) 세포에서 두드러지게 나타났다. Capsaicin 투여 후 2일군에서는 1일군에 비해 활성도가 더 저하되어 면역반응 신경세포의 총수가 37.9% (1형: 23.7%↓, 3형: 52.2%↓) 감소하였다. Capsaicin 투여 후 1주일군에서는 가장 현저한 감소를 나타내어 정상군에 비해 64.9% (1형: 54.8%↓, 2형: 19.0%↓, 3형: 80%↓) 감소하였다. Capsaicin 투여 후 4주군에서 α1B subunit의 면역활성도 는 1주군에 비해 증가되는 추세를 보이고 있었으나 정상군에 비해 면역반응 신경세포의 총수는 43.9% (1형: 23.7%↓, 2형: 17.8%↓, 3형: 56.2%↓) 감소한 상태로 남아있었다. 이상의 결과로 capsaicin 투여에 의해 성숙흰쥐의 근육층신경얼기에서 칼슘채널 α1B subunit의 면역활성도는 투 여 후 1주일까지 빠르게 저하되며 그 이후부터는 점차 정상으로 회복되나 완전한 상태로의 회복에는 4주 이상의 시간경과가 필요함을 알 수 있었다.
Abstract
This study was performed to investigate the changes of immunoreactivities of calcium channel α1B subunit in myenteric plexus of capsaicin treated adult rats. Sprague Dawley rats (about 200 g) were injected with capsaicin (50 mg/kg) subcutaneously. Ileal myenteric plexus was prepared by whole mount preparation method in 1 day, 2 days, 1 week and 4 weeks after capsaicin treated adult rats. Each myenteric plexus was reacted with NADH-TR and immunostained with α1B subunit. Myenteric ganglion cell numbers were counted by image analyzer. In control group myenteric plexus was arranged in rectangular appearance; myenteric ganglia and internodal strand were immunoreacted with α1B subunit. Immunoreactive cells were 56.0% of total myenteric neurons. Total numbers of immunoreactive cells decreased by 19.0%, 37.9% and 64.9% in 1 day, 2 days, and 1 week after capsaicn treated group respectively. In 4 weeks after capsaicin treated group, immunoreactivities of α1B subunit were increased compared to that of the 1 week after group. However total numbers of immuoreactive cells decreased by 43.9% compared to that of the control group. In conclusion, we confirmed that immunoreactivities of α1B subunit were decreased until 1 week after capsaicin treatment and recovered progressively after that time. It will take more than 4 weeks latency to recover the control immunoreactivities of α1B subunit.
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