생쥐창자 근육층신경얼기에 분포하는 vanilloid receptor type-1: 면역조직화학 및 전기생리학적 연구
Vanilloid receptor type-1 immunoreactivities in the mouse myenteric plexus: immunohistochemical and electrophysiological study
0
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
: Capsaicin은 감각성 신경원에 작용하는 강력한 흥분성 물질로 vanilloid 수용체에 결합하여 통증을 유발 하는 것으로 알려지고 있다. 뒤뿌리신경절, 삼차신경절, 결절신경절 등에서는 vanilloid receptor 제1형(VR1)의 분포 가 잘 알려져 있지만, 소화관에서는 VR1에 대한 분포가 잘 알려져 있지 않은 실정이다. 따라서 본 실험에서는 생 쥐의 작은창자와 큰창자에서 VR1의 분포를 알아내고, 창자운동의 향도잡이로 알려진 사이질세포(interstitial cells of Cajal)와 VR1과의 관계를 공초점주사레이저현미경과 전기생리학적 방법을 이용하여 알아보았다. VR1에 대한 면역조직화학염색을 실시한 결과 VR1면역양성신경세포들은 근육층신경얼기에서 주로 관찰되었다. VR1 면역양성 섬유들이 근육층신경절의 세포들을 둘러싸고 인접한 근육층신경얼기들과 연결띠를 형성하였다. 신경섬유와 달리 신경세포체에서는 매우 약한 반응만 관찰되었다. 돌림근육층과 세로근육층에서는 VR1 염색반응을 관찰할 수 없었 다. 사이질세포와의 관계를 알아보기위해 사이질세포와의 이중형광염색결과 사이질세포에서 VR1 염색반응을 관 찰할 수 없었고, 사이질세포와 VR1 양성세포사이에는 상호연관성이 없었다. 이러한 사실은 전기생리학적으로 capsaicin의 투여가 사이질세포의 전기생리학적 성질에 영향을 주지 않는 것으로 확인되었다. 이상의 결과를 토대 로 생쥐 VR1 양성신경세포들은 근육층신경얼기의 신경섬유에서 주로 관찰되었으며, 사이질세포와는 직접적 관련 이 없음을 알 수 있었다.
Abstract
The vanilloid receptor type-1(VR1) is a nonselective cation channel activated by capsaicin and can be act as mediator of chemical and physical stimuli that elicit pain. The presence of VR1 in the dorsal root, trigeminal and nodose ganglia has been firmly established, but it unclear in the mouse intestinal wall. The distribution of VR1 receptors in mouse afferent neurons innervating the intestinal tract was investigated by immunohistochemistry. Also small and large intestines were dual-labelled with antibody for VR1 and marker for interstitial cells of Cajal(c-kit). VR1-immunopositive cells were localized on fine fibers in myenteric plexus and expressed weakly myenteric ganglia. The majority of VR1- immunopositive fibers are not colocalized with or apposed to c-kit positive interstitial cells of Cajal. Also electrophysiologically capsaicin had no effect on cultured interstitial cells of Cajal. It is concluded that VR1-immunoreactive intestinal nerves are mainly distributed in myenteric plexus of murine intestinal wall, and vanillod may be not directly related to interstitial cells of Cajal in regulation of intestinal motility.
참고문헌 (43)
- S Anavi-Goffer, "Vanilloid receptor type 1-immunoreactivity is expressed by intrinsic afferent neurones in the guinea-pig myenteric plexus", Neurosci Lett, 319, pp.53~57 (2002)
- S Anavi-Goffer, "Cellular distribution of vanilloid VR1 receptor immunoreactivity in the guinea-pig myenteric plexus", Eur J Pharmacol, 1(458), pp.61~71 (2003)
- PM Anton, "low-level administration of diquat increases the nociceptive response to gastric distension in rats: role of mast cells and tachykinin receptor activation", Pain, 92, pp.219~227 (2001)
- L Bartho, "Antagonism of the effects of calcitonin gene-related peptide and of capsaicin on the guinea-pig isolated ileum by human alpha-calcitonin gene-related peptide", Neurosci Lett, 129, pp.156~159 (1991)
- L Bartho, "Tachykinin receptors are involved in the “local efferent” motor response to capsaicin in the guinea-pig small intestine and esophagus", Neuroscience, 90, pp.221~228 (1999)
- HR Berthoud, "Vagal and spinal mechanosensors in the rat stomach and colon have multiple receptive fields", Am J Physiol Regul Integr Comp Physiol, 280, pp.1371~1381 (2001)
- HR Berthoud, "Vagal afferent innervation of the rat fundic stomach: morphological characterization of the gastric tension receptor", J Comp Neurol, 319, pp.261~276 (1992)
- K Bielefeldt, "Differential effects of capsaicin on rat visceral sensory neurons", Neuroscience, 101, pp.727~736 (2000)
- SM Carlton, "capsaicin receptors increase in the inflamed rat hindpaw: a possible mechanism for peripheral sensitization", Neurosci Lett, 310, pp.53~56 (2001)
- MJ Caterina, "Impaired nociception and pain sensation in mice lacking the capsaicin receptor", Science, 288, pp.306~313 (2000)
- M Costa, "Neurochemical classification of myenteric neurons in the guinea-pig ileum", Neuroscience, 75, pp.949~967 (1996)
- AA Coutts, "Localisation of cannabinoid CB1 receptor immunoreactivity in the guinea pig and rat myenteric plexus", J Comp Neurol, 448, pp.410~422 (2002)
- D Grundy, "Neuroanatomy of visceral nociception: vagal and splanchnic afferent", Gut, 51(1), pp.2~5 (2002)
- P Holzer, "injury, repair, and adaptation in the GI tract. II. The elusive action of capsaicin on the vagus nerve", Am J Physiol, 275, pp.8~13 (1998)
- J Hammer, "capsaicin does not affect fluid and electrolyte absorption in the human jejunum but does cause pain", Gut, 43, pp.252~255 (1998)
- P Holzer, "Neural injury, repair, and adaptation in the GI tract. II. The elusive action of capsaicin on the vagus nerve", Am J Physiol, 275, pp.8~13 (1998)
- JD Huizinga, "W", kit gene required for interstitial cells of Cajal and for intestinal pacemaker activity, 1995, pp.373, 347~349
- A Kulkarni-Narla, "Opioid, cannabinoid and vanilloid receptor localization on porcine cultured myenteric neurons", Neurosci Lett, 308, pp.153~156 (2001)
- M Lemann, "Abnormal perception of visceral pain in response to gastric distension in chronic idiopathic dyspepsia The irritable stomach syndrome", Dig Dis Sci, 36, pp.124~1254 (1991)
- Y Li, "Pancreatic secretion evoked by cholecystokinin and non-cholecystokinin-dependent duodenal stimuli via vagal afferent fibres in the rat", J Physiol, 494, pp.773~782 (1996)
- YX Liu, "Duodenal acid-induced gastric relaxation is mediated by multiple pathways", Am J Physiol, 276, pp.1501~1506 (1999)
- AE Lomax, "Neurochemical classification of enteric neurons in the guinea-pig distal colon", Cell Tissue Res, 302, pp.59~72 (2000)
- CA Maggi, "Maggi Tachykinins and calcitonin gene-related peptide (Cgrp) as cotransmitters released from peripheral endings of sensory nerves", Prog Neurobiol, 45, pp.1~98 (1998)
- CF Mang, "Differential effects of anandamide on acetylcholine release in the guinea-pig ileum mediated via vanilloid and non-CB1 cannabinoid receptors", Br J Pharmacol, 134, pp.161~167 (2001)
- SJ Marsh, "The mechanism of action of capsaicin on sensory C-type neurons and their axons in vitro", Neuroscience, 23, pp.275~289 (1987)
- T Michl, "Vagal afferent signaling of a gastric mucosal acid insult to medullary, pontine, thalamic, hypothalamic and limbic, but not cortical, nuclei of the rat brain", Pain, 92, pp.19~27 (2001)
- Y Nozawa, "Distribution and characterization of vanilloid receptors in the rat stomach", Neurosci Lett, 309, pp.33~36 (2001)
- LM Patterson, "Vanilloid receptor (VR1) expression in vagal afferent neurons innervating the gastrointestinal tract", Cell Tissue Res, 31, pp.277~287 (2003)
- RJ Phillips, "and stretch receptors in gastrointestinal smooth muscle: re-evaluating vagal mechanoreceptor electrophysiology", Brain Res Rev, 34, pp.1~26 (2000)
- S Poonyachoti, "Chemical coding of neurons expressing delta- and kappa-opioid receptor and type I vanilloid receptor immunoreactivities in the porcine ileum", Cell Tissue Res, 307, pp.23~33 (2002)
- HE Raybould, "Duodenal acid-induced inhibition of gastric motility and emptying in rats.", Am J Physiol, 265, pp.540~546 (1993)
- C Savidge, "and functional characterization of the guinea-pig vanilloid receptor 1", Neuropharmacology, 43, pp.450~456 (2002)
- S Sekizawa, "Vagal esophageal receptors in anesthetized dogs:mechanical and chemical responsiveness", J Appl Physiol, 86, pp.1231~1235 (1999)
- KA Sharkey, "Visceral and somatic afferent origin of calcitonin generelated peptide immunoreactivity in the lower thoracic spinal cord of the rat", Neuroscience, 32, pp.169~179 (1989)
- KA Sharkey, "Capsaicinsensitive vagal stimulation-induced gastric acid secretion in the rat: evidence for cholinergic vagal afferents", Br J Pharmacol, 103, pp.1997~2003 (1991)
- EH South, "Capsaicin application to central or peripheral vagal fibers attenuates CCK satiety", Peptides, 9, pp.601~612 (1988)
- C Sternini, "Calcitonin gene-related peptidecontaining neurons supplying the rat digestive system: differential distribution and expression pattern", Somatosens Mot Res, 9, pp.45~59 (1992)
- G Such, "Axonal effects of capsaicin: an electrophysiological study", Acta Physiol Hung, 67, pp.53~63 (1986)
- A Szallasi, "Vanilloid receptors: new insights enhance potential as a therapeutic target", Pain, 68, pp.195~208 (1996)
- A Szallasi, "Vanilloid (capsaicin) receptors in the rat: distribution in the brain, regional differences in the spinal cord, axonal transport to the periphery, and depletion by systemic vanilloid treatment", Brain Res, 703, pp.175~183 (1995)
- M Tominaga, "Capsaicin receptor in the pain pathway", Jpn J Pharmacol, 83, pp.20~24 (2000)
- RJ Traub, "Differential c-fos expression in the nucleus of the solitary tract and spinal cord following noxious gastric distention in the rat", Neuroscience, 74, pp.873~884 (1996)
- VP Zagorodnyuk, "Intraganglionic laminar endings are mechano-transduction sites of vagal tension receptors in the guinea-pig stomach", J Physiol, 534, pp.255~268