보리, 치커리, 여주, 바나바 혼합추출물의 in vitro 항산화 및 항당뇨 활성 평가
Antioxidant and Antidiabetic Activities of a Mixed Extract of Barley, Chicory, Bitter Melon, and Banaba in vitro
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
운동에 의해 발생하는 일시적 산화스트레스와 당뇨병 환자에서 나타나는 만성적 산화스트레스는세포손상, 염증반응, 미토콘드리아기능저하등을유발하여재활과정에서 근기능회복과대사조절을저해하는주요요인으로알려져있다. 이러한산화적손상은항산화 방어체계의불균형과함께혈당조절장애를심화시키므로, 효과적인항산화및항당뇨소재의 발굴은재활영양관리측면에서매우중요하다. 본연구에서는보리(Hordeum vulgare) 곡립, 치커리(Cichorium intybus) 뿌리, 여주(Momordica charantia) 과육, 바나바(Lagerstroemia speciosa) 잎혼합추출물(HCML)의항산화및항당뇨활성을평가하였다. HCML의항산화효과는DPPH, ABTS radical 소거능및환원력분석을통해확인한결과, 농도의존적으로활성이증가하였으며 특히500 μg/mL HCML은ascorbic acid와비슷한수준으로DPPH radical을소거하는것으로 나타났다. 또한α-amylase 및α-glucosidase 억제활성을측정한결과, HCML은농도의존적으로 소화효소를저해하였고, 특히500 μg/mL HCML은acarbose와비슷한수준으로α-glucosidase를 억제하여혈당상승억제에기여할가능성을나타냈다. 이러한결과는HCML이산화적손상 완화와탄수화물대사조절을동시에개선할수있는복합기능성소재임을시사한다. 특히, 운동후 회복기또는만성질환자의산화스트레스완화및혈당안정화를통한대사항상성유지에도움을줄 수있을것으로기대된다. 따라서, 본연구는HCML을활용한기능성음료및재활영양보조제 개발을위한기초자료로서의미를가지며, 향후임상적적용을위한기초자료를제공한다.
Abstract
Transient oxidative stress induced by exercise and chronic oxidative stress observed in diabetic patients are known to cause cellular damage, inflammatory responses, and mitochondrial dysfunction, thereby impeding muscle recovery and metabolic regulation during rehabilitation. Such oxidative damage, accompanied by an imbalance in the antioxidant defense system, exacerbates glucose dysregulation; therefore, the discovery of effective antioxidant and antidiabetic agents is crucial for nutritional management in rehabilitation. In this study, the antioxidant and antidiabetic activities of a mixed extract (HCML) composed of barley (Hordeum vulgare) grains, chicory (Cichorium intybus) roots, bitter melon (Momordica charantia) fruit, and banaba (Lagerstroemia speciosa) leaves were evaluated. The antioxidant effects of HCML were assessed using DPPH and ABTS radical scavenging assays, as well as a reducing power analysis. The results showed a concentration-dependent increase in antioxidant activity, and 500 μg/mL of HCML exhibited a DPPH radical scavenging effect comparable to that of ascorbic acid. Furthermore, α-amylase and α-glucosidase inhibitory assays demonstrated that HCML inhibited digestive enzymes in a concentration-dependent manner. Notably, 500 μg/mL HCML showed an α-glucosidase inhibitory effect similar to that of acarbose, suggesting its potential to suppress postprandial blood glucose elevation. These findings indicate that HCML is a multifunctional bioactive material capable of simultaneously alleviating oxidative stress and improving carbohydrate metabolism. In particular, it may contribute to maintaining metabolic homeostasis through oxidative stress reduction and glycemic control during post-exercise recovery or in individuals with chronic metabolic disorders. Therefore, this study provides fundamental data supporting the development of functional beverages or nutritional supplements applicable to rehabilitation and clinical nutrition management.