당뇨유발 흰쥐에 있어 여주분말의 항당뇨 및 항산화작용에 대한연구
Antidiabetic and Antioxidative Effects of Bitter Melon on Streptozotocin-induced Diabetic Rats
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본 연구의 목적은 Streptozotocin에 의한 당뇨 유발 흰쥐에대한 여주 분말의 항당뇨병 및 항산화능 효과를 알아보기 위한것이다. 정상 및 대조군에 AIG-93M식이를 공급하였고, 여주첨가 군에는 1%, 2%, 3%의 여주 분말을 공급하였다. 2주 후Streptozotocin 투여로 당뇨를 유발하였다. 동물 희생 후 혈당조절과 항산화능을 분석하였다. 여주 첨가군의 혈당 수준은 모두 대조군보다 낮았으며, 2% 여주 첨가군은 대조군보다 유의하게 낮은 결과를 보였다. 2%, 3% 여주 첨가군의 혈청 트리글리세라이드와 고밀도지질단백질-콜레스테롤 수치는 대조군보다 유의하게 낮은 결과가 나왔다. 당뇨 유발 군의 혈청 인슐린은 정상군보다 유의하게 낮았다. 당화혈색소는 2% 및 3% 여주첨가 군이 대조군보다 유의하게 낮은 결과를 보였다. 간 조직에서 항산화 효소의 수치는 2% 여주첨가군이 대조군보다 유의하게 높게나타났다. 이러한 결과는 당뇨병 예방 및 당뇨병 치료를 위한 여주 분말의 항당뇨병 및 항산화 효과를 나타내는 것으로 보여진다.
Abstract
The purpose of this study was to determine the antidiabetic and antioxidative effects of Bitter melon on streptozotocin-induced diabetic rats. The normal and the control groups were fed an AIG -93M diet, and the Bitter melon groups were fed 1%, 2% and 3% Bitter melon powder. After two weeks, the control and the experimental group were induced to a diabetic state with the administration of streptozotocin. The blood glucose control and antioxidant activity were analyzed after the animals were sacrificed. The blood glucose levels of all the Bitter melon groups were lower than those of the control group, and the 2% Bitter melon group showed significantly lower blood glucose levels than those of the control group. Serum Triglyceride and HDL-cholesterol of the 2%, and 3% Bitter melon groups were significantly lower than those of the control group. The total cholesterol levels of the bitter melon groups were significantly lower than those of the control group. The serum insulin levels of the induced groups were significantly lower than those of the normal group. The HbA1c levels of the 2% and 3% Bitter melon groups were significantly lower than those of the control group. For the level of antioxidant enzymes in the liver tissues, the 2% Bitter melon group was significantly higher than that of the control group. These results show the antidiabetic and antioxidative effects of Bitter melon for the prevention and treatment of diabetes.
참고문헌 (33)
- 정재황, "C57BL", 6J db, 대한수의학회지, pp.2008, 48(3)[DOI]
- Griendling KK, "Oxidative stress and cardiovascular injury : Part II : animal and human studies", Circulation, 108, pp.2034~2040 (2003)[DOI]
- Kim JY, "Effect of the antioxidants on the diabetic complications", J Vet Clin, 18, pp.374~379 (2001)
- 김보현, "산화스트레스에 의한 당뇨병성 혈관합병증의 발생기전", Endocrinology and Metabolism, 21(6), pp.448~459 (2006), 대한내분비학회
- Urano S, "Vitamin E and the susceptibility of erythrocytes and reconstituted liposomes to oxidative stress in aged diabetics", Lipids, 26, pp.58~61 (1991)[DOI]
- Ha AW, "The study of lipid-per-oxidation, antioxidant enzymes, and the antioxidant vitamin in NIDDM patients with micro vascular-diabetic complication", Korean J Nutr, 32, pp.17~23 (1999)
- Shetty AK, "Effect of bitter gourd(Momordica charantia)on glycaemic status in streptozotocin induced diabetic rats", Plant Foods Hum Nutr, 60, pp.109~112 (2005)[DOI]
- Liva Harinantenaina, "Momordica charantia Constituents and Antidiabetic Screening of the Isolated Major Compounds", CHEMICAL & PHARMACEUTICAL BULLETIN, 54(7), pp.1017~1021 (2006), Pharmaceutical Society of Japan[DOI]
- Krawinkel MB, "Bitter gourd(Momordica charantia) : A dietary approach to hyperglycemia", Nutr Rev, 64, pp.331~337 (2006)[DOI]
- Schmourlo G, "Screening of antifungal agents using ethanol precipitation and bioautography of medicinal and food plants", J Ethnopharmacol, 96, pp.563~568 (2005)[DOI]
- Budrat P, "Extraction of phenolic compounds from fruits of bitter melon (Momordica charantia) with subcritical water extraction and antioxidant activities of these extracts", CMU J Nat Sci, 35, pp.123~130 (2008)
- Lee YB, "The research for production of isoflavone metabolite derived from soy isoflavone and the development of medical food for treatment of menopausal symptoms", Dr Chung’s Food (2012)
- Lowry OH, "Protein measurement with the Folin phenol reagent", J Biol Chem, 193, pp.265~275 (1951)
- Mihara M, "Determination of malonaldehyde precursor in tissues by thiobarbituric acid test", Anal Biochem, 86, pp.271~278 (1978)[DOI]
- Aebi H, "Catalase in vitro", Methods Enzymol, 105, pp.121~126 (1984)[DOI]
- Habig WH, "Assays for differentiation of glutathione S-transferases", Methods Enzymol, 77, pp.398~405 (1981)[DOI]
- Crapo JD, "Preparation and assay of superoxide dismutases", Methods Enzymol, 53, pp.382~393 (1978)[DOI]
- Ellman GL, "Tissue sulfhydryl groups", Arch Biochem Biophys, 82, pp.70~77 (1959)[DOI]
- 심기철, "선행된 천마식이가 당뇨유발백서의 항산화효과 및 병태생리학적 인자에 미치는 영향", 동의생리병리학회지, 22(6), pp.1537~1543 (2008), 한의병리학회
- Joseph B, "Antidiabetic effects of Momordica charantia(bitter melon)and its medicinal potency", Asian Pac J Trop Dis, 3, pp.92~102 (2013)[DOI]
- Raish M, "Momordica charantia polysaccharides ameliorate oxidative stress, hyperlipidemia, inflammation, and apoptosis during myocardial infarction by inhibiting the NF-kappaB signaling pathway", Int J Biol Macromol, 97, pp.544~551 (2017)
- Bai J, "Response of gut microbiota and inflammatory status to bitter melon (Momordica charantia L.) in high fat diet induced obese rats", J Ethnopharmacol, 194, pp.717~726 (2016)
- Bao B, "Momordica charantia(Bitter Melon)reduces obesity-associated macrophage and mast cell infiltration as well as inflammatory cytokine expression in adipose tissues", PloS One, 8, pp.E84075~ (2013)
- 박은교, "Jaceosidin이 streptozotocin으로 유도된 당뇨 쥐의 혈당강하 및 항산화능에 미치는 영향", Journal of Nutrition and Health, 47(5), pp.313~320 (2014), 한국영양학회[DOI]
- Kahn CR, "The molecular mechanism of insulin action", Ann Rev Med, 36, pp.429~451 (1985)[DOI]
- 임숙자, "택사 butanol 분획물과 vitamin E의 투여가 streptozotocin 유발 당뇨 흰쥐의 혈당 및 혈장 지질수준에 미치는 영향", 한국식품과학회지, 35(4), pp.713~719 (2003), 한국식품과학회
- Allen DW, "Observations on the chromatographic heterogeneity of normal adult and fetal human hemoglobin : a study of the effects of crystallization and chromatography on the heterogeneity and isoleucine content", J Am Chem Soc, 80, pp.1628~1634 (1958)[DOI]
- Kim YC, "Establishment of functionality evaluation system for antioxidant of health functional food", pp.2003
- 김옥경, "산수유 추출물이 Streptozotocin으로 유발된 흰쥐의 항당뇨 및 항산화 작용에 미치는 효과", 한국응용과학기술학회지, 22(2), pp.157~167 (2005), 한국응용과학기술학회
- Cho SY, "Alternation of hepatic antioxidant enzyme activities and lipid profile in streptozotocin-induced diabetic rats by supplementation of dandelion water extract", Clin Chim Acta, 317, pp.109~117 (2002)[DOI]
- 방미애, "당뇨 유발쥐에서 인동초의 섭취가 혈청지질과 혈당 및 항산화효소계에 미치는 영향", 한국식생활문화학회지, 17(4), pp.377~386 (2002), 한국식생활문화학회
- Toxic effects determination by target organ, "Park SY", National Institute of Environmental Research, 123~132, pp.Report No. NIERGP2016- 038 (2016)
- West KM, "The role of circulating glucose and triglyceride concentrations and their interactions with other"risk factors"as determinants of arterial disease in nine diabetic population samples from the WHO multinational study", Diabetes Care, 6, pp.361~369 (1983)