Rhodotorula rubra의 항원특성에 관한 연구

Abstract

Antigenicity of Rhodotorula rubra isolated from pulmonary tissue of pulmonary tuberculosis patients was studied by means of agglutination reaction with R. rubra whole cell antiserum. And the serological reactivity of crude polysaccharide from R. rubra, Candida albicans, Candida tropicalis, Candida glabrata, and Saccharomyces cerevisiae ATCC 26603 with antiserum to R. rubra whole cell was studied by means of immunodiffusion test. R. rubra showed stationary phase after 48h when it was cultured in GYEP broth. While agglutinogen titer was 1:64 at lag phase, agglutinogen titer was 1:256 after 20h. After growth of R. rubra on different 11 media, nutritional environment showed similar agglutination reactivity. The agglutinogen titer of C. albicans, C. tropicalis, C. glabrata, which were isolated from patient's expectoration, to R. rubra antiserum by means of agglutination reaction were 1:16, respectively. But, Sacch. cerevisiae ATCC26603 was negative. Those results were lower than that of R. rubra agglutinogen titer 1:256. As a result of immunodiffusion test with crude polysaccharide extracted from cell wall of R. rubra, C. albicans, C. tropicalis, C. glabrata, Sacch. cerevisiae ATCC26603, precipitin line was found only with R. rubra, of which antibody titer was 8.