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Ribosomal DNA Intergenic Spacer의 Polymorphism을 이용한 Trichophyton rubrum의 균주간 구분

Strain Differentiation of Trichophyton rubrum Using Polymorphism of rDNA Intergenic Spacer

신동훈|김기홍|성준제|최종수
대한의진균학회 (The Korean Society for Medical Mycology)|2004.12|대한의진균학회지 (Journal of Mycology and Infection)|제9권 제4호|pp. 206-213

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참고문헌

주제분류미생물학

초록

Abstract

Background: In Korea, Trichophyton (T.) rubrum is occupied more than 80% of causative fungi of dermatophytosis. Strain differentiation of T. rubrum is essential for epidermiologic study. Objective: The aim of this study is to develope the primers for amplification of rDNA intergenic spacer(IGS) to detect polymorphism of T. rubrum. Methods: The primers were designed from 25S and 18S of rDNA, and were applied to 20 strains of T. rubrum, which included 1 standard strain and 19 clinical isolates. Results: Primers for amplification of polymorphic rDNA IGS were designed from the 3’end of the 25S(primer ANID25-3, 5’-GACAGGTTAGTTTTACCCTACTGA-3’) and the 5’end of the 18S (TR18-2R, 5’-ATCTAATAAATACACCCCTTCCGA-3’). PCR condition was adjusted for detecting polymorphism. Best results were obtained at 55℃ for annealing temperature and 3 minutes for extension time. Eight bands sized as 1.1, 2.4, 2.7, 2.9, 3.2, 3.8, 5 kb were amplified with PCR using the primers. With 4 bands sized 2.7, 2.9, 3.2 and 3.8 kb, 20 strains of T. rubrum could be grouped into 6 subtypes. Conclusion: The PCR fingerprinting with the primers for rDNA IGS was able to differentiated strains of T. rubrum, and it can be applied in clinical and epidemiologic studies. The primer could be applied to other fungi with unknown sequences.

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Ribosomal DNA Nontranscribed Spacer의 분석을 이용한 동일 환자의 두 병소에서 분리된 Trichophyton rubrum의 균주간 구분김기홍, 김현철, 신동훈, 최종수대한의진균학회 · 대한의진균학회지2002 · 제7권 제2호rRNA Spacer 부위를 이용한 Candida의 균종동정김혜련, 노병인, 이미경대한의진균학회 · 대한의진균학회지2006 · 제11권 제1호Malassezia 효모균 동정에 26S rDNA PCR-RFLP기법의 적용안규중, 이양원, 임상희대한의진균학회 · 대한의진균학회지2006 · 제11권 제3호PCR-reverse Blot Hybridization Assay을 이용한 피부사상균의 동정김성현, 김영권, 김현정, 박제섭, 방혜은, 안규중, 왕혜영, 원영호, 이장호, 이혜영, 진현우, 최연임대한의진균학회 · 대한의진균학회지2011 · 제16권 제3호국내 최초로 분리된 Arthroderma benhamiae의 진균학적 및 분자생물학적 관찰상영호, 서순봉, 전재복, 정상립, 최종수대한의진균학회 · 대한의진균학회지2004 · 제9권 제1호Identification of Dermatophytes Using Multiplex Polymerase Chain Reaction안규중, 이양원, 최용범, Ji Young Kim대한피부과학회 · Annals of Dermatology2011 · 제23권 제3호

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