본문 바로가기
KERD
논문KCI 등재

Hypoglycemic and hyporlipidemic effects of unsaponifiable matter from okra seed in diabetic rats

2

조회

0

다운로드

0

인용

0

참고문헌

키워드

초록

AbstractBACKGROUND/OBJECTIVESOkra seed is a rich source of various nutritional and bioactive constituents, but its mechanism of action is still unclear. The aim of this study was to evaluated the effects on glucose uptake and serum lipid profiles of unsaponifiable matter (USM) from okra seed in adipocytes and diabetic animal models. MATERIALS/METHODSUSM was prepared from okra seed powder by saponification. The contents of phytosterols and vitamin E in USM were measured. 3T3-L1 preadipocytes were cultured for 6 days with different concentrations of USM (0–200 μg/mL). The diabetic rats were administered with or without USM for 5 wk. RESULTSIn the USM, the contents of phytosterols and vitamin E were 394.13 mg/g USM and 31.16 mg/g USM, respectively. USM showed no cytotoxicity and led to an approximately 1.4-fold increase in glucose uptake in 3T3-L1 adipocytes. The treatment of USM also increased the expressions of peroxisome proliferator-activated receptor-γ and glucose transporter-4 in a dose-dependent manner in adipocytes. The body weight change was not significantly different in all diabetic rats. However, blood glucose and the weights of liver and adipose tissues were significantly reduced compared to those in the control diabetic rats. Treatment with USM decreased the levels of triglycerides, total cholesterol, and low-density lipoprotein cholesterol compared to the control group. The USM group also showed significantly decreased atherogenic indices and cardiac risk factors. CONCLUSIONThese results suggest that USM from okra seed improves the hypoglycemic and hypolipidemic effects in diabetic rats, and provides valuable information for improving the functional properties of okra seed.

Abstract

AbstractBACKGROUND/OBJECTIVESOkra seed is a rich source of various nutritional and bioactive constituents, but its mechanism of action is still unclear. The aim of this study was to evaluated the effects on glucose uptake and serum lipid profiles of unsaponifiable matter (USM) from okra seed in adipocytes and diabetic animal models. MATERIALS/METHODSUSM was prepared from okra seed powder by saponification. The contents of phytosterols and vitamin E in USM were measured. 3T3-L1 preadipocytes were cultured for 6 days with different concentrations of USM (0–200 μg/mL). The diabetic rats were administered with or without USM for 5 wk. RESULTSIn the USM, the contents of phytosterols and vitamin E were 394.13 mg/g USM and 31.16 mg/g USM, respectively. USM showed no cytotoxicity and led to an approximately 1.4-fold increase in glucose uptake in 3T3-L1 adipocytes. The treatment of USM also increased the expressions of peroxisome proliferator-activated receptor-γ and glucose transporter-4 in a dose-dependent manner in adipocytes. The body weight change was not significantly different in all diabetic rats. However, blood glucose and the weights of liver and adipose tissues were significantly reduced compared to those in the control diabetic rats. Treatment with USM decreased the levels of triglycerides, total cholesterol, and low-density lipoprotein cholesterol compared to the control group. The USM group also showed significantly decreased atherogenic indices and cardiac risk factors. CONCLUSIONThese results suggest that USM from okra seed improves the hypoglycemic and hypolipidemic effects in diabetic rats, and provides valuable information for improving the functional properties of okra seed.

참고문헌 (45)

  1. Devalaraja S, "Exotic fruits as therapeutic complements for diabetes, obesity and metabolic syndrome", Food Res Int, 44, pp.1856~1865 (2011)
  2. Lois K, "Obesity and diabetes", Endocrinol Nutr, 56(Suppl 4), pp.38~42 (2009)
  3. Nawrocki AR, "Keynote review : the adipocyte as a drug discovery target", Drug Discov Today, 10, pp.1219~1230 (2005)
  4. Vázquez-Vela ME, "White adipose tissue as endocrine organ and its role in obesity", Arch Med Res, 39, pp.715~728 (2008)
  5. Booth A, "Adipose tissue : an endocrine organ playing a role in metabolic regulation", Horm Mol Biol Clin Investig, 26, pp.25~42 (2016)
  6. Chukwuma CI, "Erythritol reduces small intestinal glucose absorption, increases muscle glucose uptake, improves glucose metabolic enzymes activities and increases expression of Glut-4 and IRS-1 in type 2 diabetic rats", Eur J Nutr, 57, pp.2431~2444 (2018)
  7. Zhu K, "A newly identified polysaccharide from Ganoderma atrum attenuates hyperglycemia and hyperlipidemia", Int J Biol Macromol, 57, pp.142~150 (2013)
  8. Parikh NH, "Indigenous plant medicines for health care : treatment of diabetes mellitus and hyperlipidemia", Chin J Nat Med, 12, pp.335~344 (2014)
  9. Shaik Mohamed Sayed UF, "Natural products as novel anti-obesity agents : insights into mechanisms of action and potential for therapeutic management", Front Pharmacol, 14, pp.1182937~ (2023)
  10. Karri S, "Natural anti-obesity agents and their therapeutic role in management of obesity : a future trend perspective", Biomed Pharmacother, 110, pp.224~238 (2019)
  11. Islam MT, "Phytochemical information and pharmacological activities of Okra(Abelmoschus esculentus) : a literature-based review", Phytother Res, 33, pp.72~80 (2019)
  12. Elkhalifa AE, "Okra(Abelmoschus esculentus)as a potential dietary medicine with nutraceutical importance for sustainable health applications", Molecules, 26, pp.696~ (2021)
  13. Afinisha Deepam LS, "Effect of saponification on composition of unsaponifiable matter in rice bran oil", J Oleo Sci, 61, pp.241~247 (2012)
  14. Sharma RD, "Hypocholesterolemic activity of unsaponifiable matter of rice bran oil", Indian J Med Res, 85, pp.278~281 (1987)
  15. Lee H, "Protective effects of unsaponifiable matter from perilla seed meal on UVB-induced damages and the underlying mechanisms in human skin fibroblasts", Antioxidants(Basel), 8, pp.644~ (2019)
  16. Seo D, "Evaluation of bioactive compounds contents and biological activities of okra seeds oils", J Korean Soc Food Cult, 35, pp.392~399 (2020)
  17. Shin EC, "Commercial peanut(Arachis hypogaea L. )cultivars in the United States : phytosterol composition", J Agric Food Chem, 58, pp.9137~9146 (2010)
  18. Yeon J, "Comparison of phytochemical contents and cytoprotective effects of different rice bran extracts from Indica and Japonica rice cultivars", Prev Nutr Food Sci, 25, pp.432~439 (2020)
  19. Kim Y, "Esculetin inhibits the inflammatory response by inducing heme oxygenase-1 in cocultured macrophages and adipocytes", Food Funct, 5, pp.2371~2377 (2014)
  20. Kim Y, "Effect of(-)-epigallocatechin-3-gallate on anti-inflammatory response via heme oxygenase-1 induction during adipocyte-macrophage interactions", Food Sci Biotechnol, 25, pp.1767~1773 (2016)
  21. Ham H, "Effect of rice bran unsaponifiables on high-fat diet-induced obesity in mice", J Food Biochem, 39, pp.673~681 (2015)
  22. Zhao L, "A combination of quercetin and resveratrol reduces obesity in high-fat diet-fed rats by modulation of gut microbiota", Food Funct, 8, pp.4644~4656 (2017)
  23. Peng J, "Quercetin improves glucose and lipid metabolism of diabetic rats : involvement of Akt signaling and SIRT1", J Diabetes Res, 2017, pp.3417306~ (2017)
  24. Kang NH, "Risk of cardiovascular disease is suppressed by dietary supplementation with protamine and chitooligosaccharide in Sprague-Dawley rats", Mol Med Rep, 7, pp.127~133 (2013)
  25. Expert Panel on Detection, "Executive Summary of The Third Report of The National Cholesterol Education Program(NCEP)Expert Panel on Detection, Evaluation, And Treatment of High Blood Cholesterol In Adults(Adult Treatment Panel III)", JAMA, 285, pp.2486~2497 (2001)
  26. de Jong A, "Metabolic effects of plant sterols and stanols(Review)", J Nutr Biochem, 14, pp.362~369 (2003)
  27. Tanaka M, "Identification of five phytosterols from Aloe vera gel as anti-diabetic compounds", Biol Pharm Bull, 29, pp.1418~1422 (2006)
  28. Ham H, "Protective effects of unsaponifiable matter from rice bran on oxidative damage by modulating antioxidant enzyme activities in HepG2 cells", Lebensm Wiss Technol, 61, pp.602~608 (2015)
  29. An M, "Unsaponifiable matter from wheat bran cultivated in Korea inhibits hepatic lipogenesis by activating AMPK pathway", Foods, 12, pp.4016~ (2023)
  30. Comitato R, "Tocotrienols: a family of molecules with specific biological activities", Antioxidants(Basel), 6, pp.93~ (2017)
  31. Paolisso G, "Pharmacologic doses of vitamin E improve insulin action in healthy subjects and non-insulin-dependent diabetic patients", Am J Clin Nutr, 57, pp.650~656 (1993)
  32. Hwang SL, "Beneficial effects of beta-sitosterol on glucose and lipid metabolism in L6 myotube cells are mediated by AMP-activated protein kinase", Biochem Biophys Res Commun, 377, pp.1253~1258 (2008)
  33. Sujatha S, "Biological evaluation of(3β)-STIGMAST-5-EN-3-OL as potent anti-diabetic agent in regulating glucose transport using in vitro model", Int J Diabetes Mellit, 2, pp.101~109 (2010)
  34. Balbi ME, "Antioxidant effects of vitamins in type 2 diabetes : a meta-analysis of randomized controlled trials", Diabetol Metab Syndr, 10, pp.18~ (2018)
  35. Prabhakar PK, "Synergistic effect of phytochemicals in combination with hypoglycemic drugs on glucose uptake in myotubes", Phytomedicine, 16, pp.1119~1126 (2009)
  36. Wilson TA, "Rice bran oil and oryzanol reduce plasma lipid and lipoprotein cholesterol concentrations and aortic cholesterol ester accumulation to a greater extent than ferulic acid in hypercholesterolemic hamsters", J Nutr Biochem, 18, pp.105~112 (2007)
  37. Zhu Y, "Phytochemicals in whole grain wheat and their health-promoting effects", Mol Nutr Food Res, 61, pp.1600852~ (2017)
  38. Goldberg DM, "Serum enzyme changes as evidence of liver reaction to oral alcohol", Gastroenterology, 49, pp.256~261 (1965)
  39. Asagba SO, "Biochemical changes in diabetic rats treated with ethanolic extract of Chrysophyllum albidum fruit-skin", J Basic Appl Zool, 80, pp.42~ (2019)
  40. Kannel WB, "Risk factors in coronary heart disease : an evaluation of several serum lipids as predictors of coronary heart disease; the Framingham study", Ann Intern Med, 61, pp.888~899 (1964)
  41. Gordon T, "Lipoproteins, cardiovascular disease, and death : the Framingham study", Arch Intern Med, 141, pp.1128~1131 (1981)
  42. Stamler J, "Prevalence and prognostic significance of hypercholesterolemia in men with hypertension. Prospective data on the primary screenees of the multiple risk factor intervention trial", Am J Med, 80, pp.33~39 (1986)
  43. Babu S, "Beta-sitosterol attenuates insulin resistance in adipose tissue via IRS-1", Akt mediated insulin signaling in high fat diet and sucrose induced type-2 diabetic rats, 2020, pp.873, 173004~
  44. Krishnan M, "Effect of β-sitosterol on insulin receptor, glucose transporter 4 protein expression and glucose oxidation in the gastrocnemius muscle of high fat diet induced type-2 diabetic experimental rats", Indian J Pharm Educ Res, 55, pp.s479~91 (2021)
  45. Shirpoor A, "Protective effect of vitamin E against diabetes-induced oxidized LDL and aorta cell wall proliferation in rat", Iran Biomed J, 19, pp.117~123 (2015)

관련 논문AI추천

Attenuation of Diabetic Conditions by Sida rhombifolia in Moderately Diabetic Rats and Inability to Produce Similar Effects in Severely Diabetic in RatsPadmaja Chaturvedi, Tebogo Elvis Kwape대한약침학회 · Journal of Pharmacopuncture2015 · 제18권 제4호Hypoglycemic Activity of the Hexane Extract of Oriental Melon (Cucumis melo L. var. makuwa Makino) Seeds and Its Active Compounds강영화, 김혜숙, 서전규, 최보람, Enning Xu, Lei Chen, Shaonan Yang한국자원식물학회 · 한국자원식물학회지2014 · 제27권 제6호Hypolipidemic effect of Salicornia herbacea in animal model of type 2 diabetes mellitus김미은, 김정인, 서영완, 소현아, 이수경, 조자임, 조창우, 황지연한국영양학회 · Nutrition Research and Practice2007 · 제1권 제4호Antihyperlipidemic Activity of the Ethyl-acetate Fraction of Stereospermum Suaveolens in Streptozotocin-induced Diabetic RatsBalasubramanian Thirumalaisamy, Karthikeyan Marimuthu, Senthilkumar Gnanavadevel Prabhakaran, Tapan Kumar Chatterjee대한약침학회 · Journal of Pharmacopuncture2013 · 제16권 제3호Anti-diabetic Effect of Opuntia humifusa Extracts in Diabetic db/db Mice유설, 유솔, 김경희, 김재곤, 박영미, 신동엽, 유경훈, 이학용한국약용작물학회 · 한국약용작물학회지2024 · 제32권 제4호Dual Mechanistic Anti-Obesity Effects of Red Okra and Diospyros lotus Mixture via Fat Binding and AMPK-Mediated Lipid MetabolismChoi Yeo-Jin, Kang Wan Seok, Kim Eun, Kim Jin Seok, Kim Sunoh, Kim Youngbae, Lee Ki Hoon, Son Seon ah한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2025 · 제35권

이 간행물의 다른 논문 (10건)