Liquid collagen from freshwater fish skin ameliorates hydration, roughness and elasticity in photo-aged skin: a randomized, controlled, clinical study
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AbstractBACKGROUND/OBJECTIVESCollagen is commonly used in diverse forms as a functional component in skincare products. On the other hand, the effects of collagen on human skin are controversial. Dietary collagen hydrolysates from freshwater Pangasius hypophthalmus fish skin ameliorated photo-aged skin of hairless mice. This study conducted a randomized, double-blind, placebo-controlled clinical trial to determine if liquid fish collagen (Collagen-Tripep20™, Tripep20) as a drink strengthens skin health and quality. SUBJECTS/METHODSIn this clinical trial, 85 subjects aged 35–60 yrs were diagnosed with photo-aged skin. Eighty-five subjects were randomized to receive either Tripep20 (n = 44) or placebo (n = 41). Seventy-eight subjects fully participating for a 12-week period consumed 1,000 mg of Tripep20 (n = 41) or placebo (n = 37) in a 50-mL bottle as a daily drink. The intend-to-treat and per-protocol populations were 85 and 78, respectively. Skin hydration, wrinkles, and elasticity were assessed at 0 (baseline), 6, and 12 weeks during the study period. RESULTSSkin hydration in the Tripep20 group was significantly higher from 6 weeks (P < 0.001) than the baseline. After 12 weeks, the Crow’s-feet visual score and skin roughness (Ra, Rq, and Rmax) were significantly improved in the Tripep20 group than in the placebo group (P < 0.05). Consuming liquid collagen Tripep20 greatly enhanced skin elasticity (Gross R2, Net R5, and Biological elasticity R7) in 6 weeks compared to the placebo group. The Tripep20 group showed a significant increase in skin elasticity from the baseline after 6 and 12 weeks (P < 0.001). Neither abnormal symptoms nor adverse events were encountered during the study period in subjects ingesting Tripep20 or placebo. The changes in parameters related to hematology and clinical chemistry were within the normal ranges. CONCLUSIONOral consumption of liquid collagen Tripep20 was safe and well‐tolerated. The results of this study show that freshwater fish‐derived liquid collagen Tripep20 can be used as a healthy functional food ingredient to improve skin moisturizing, anti-wrinkling, and elasticity in an aging population.
Abstract
AbstractBACKGROUND/OBJECTIVESCollagen is commonly used in diverse forms as a functional component in skincare products. On the other hand, the effects of collagen on human skin are controversial. Dietary collagen hydrolysates from freshwater Pangasius hypophthalmus fish skin ameliorated photo-aged skin of hairless mice. This study conducted a randomized, double-blind, placebo-controlled clinical trial to determine if liquid fish collagen (Collagen-Tripep20™, Tripep20) as a drink strengthens skin health and quality. SUBJECTS/METHODSIn this clinical trial, 85 subjects aged 35–60 yrs were diagnosed with photo-aged skin. Eighty-five subjects were randomized to receive either Tripep20 (n = 44) or placebo (n = 41). Seventy-eight subjects fully participating for a 12-week period consumed 1,000 mg of Tripep20 (n = 41) or placebo (n = 37) in a 50-mL bottle as a daily drink. The intend-to-treat and per-protocol populations were 85 and 78, respectively. Skin hydration, wrinkles, and elasticity were assessed at 0 (baseline), 6, and 12 weeks during the study period. RESULTSSkin hydration in the Tripep20 group was significantly higher from 6 weeks (P < 0.001) than the baseline. After 12 weeks, the Crow’s-feet visual score and skin roughness (Ra, Rq, and Rmax) were significantly improved in the Tripep20 group than in the placebo group (P < 0.05). Consuming liquid collagen Tripep20 greatly enhanced skin elasticity (Gross R2, Net R5, and Biological elasticity R7) in 6 weeks compared to the placebo group. The Tripep20 group showed a significant increase in skin elasticity from the baseline after 6 and 12 weeks (P < 0.001). Neither abnormal symptoms nor adverse events were encountered during the study period in subjects ingesting Tripep20 or placebo. The changes in parameters related to hematology and clinical chemistry were within the normal ranges. CONCLUSIONOral consumption of liquid collagen Tripep20 was safe and well‐tolerated. The results of this study show that freshwater fish‐derived liquid collagen Tripep20 can be used as a healthy functional food ingredient to improve skin moisturizing, anti-wrinkling, and elasticity in an aging population.
참고문헌 (45)
- Chambers ES, "Skin barrier immunity and ageing", Immunology, 160, pp.116~125 (2020)
- Kelly RI, "The effects of aging on the cutaneous microvasculature", J Am Acad Dermatol, 33, pp.749~756 (1995)
- Chung JH, "Differential effects of photoaging vs intrinsic aging on the vascularization of human skin", Arch Dermatol, 138, pp.1437~1442 (2002)
- Ichihashi M, "Photoaging of the skin", Anti-aging Medicine, 6, pp.46~59 (2009)
- Pyun HB, "Effects of collagen tripeptide supplement on photoaging and epidermal skin barrier in UVB-exposed hairless mice", Prev Nutr Food Sci, 17, pp.245~253 (2012)
- Averbeck M, "Differential regulation of hyaluronan metabolism in the epidermal and dermal compartments of human skin by UVB irradiation", J Invest Dermatol, 127, pp.687~697 (2007)
- Quan T, "Role of age-associated alterations of the dermal extracellular matrix microenvironment in human skin aging : a mini-review", Gerontology, 61, pp.427~434 (2015)
- Shoulders MD, "Collagen structure and stability", Annu Rev Biochem, 78, pp.929~958 (2009)
- Ramshaw JA, "Gly-X-Y tripeptide frequencies in collagen : a context for host-guest triple-helical peptides", J Struct Biol, 122, pp.86~91 (1998)
- Yazaki M, "Oral ingestion of collagen hydrolysate leads to the transportation of highly concentrated Gly-Pro-Hyp and its hydrolyzed form of Pro-Hyp into the bloodstream and skin", J Agric Food Chem, 65, pp.2315~2322 (2017)
- Sontakke SB, "Orally available collagen tripeptide : enzymatic stability, intestinal permeability, and absorption of Gly-Pro-Hyp and Pro–Hyp", J Agric Food Chem, 64, pp.7127~7133 (2016)
- Aito-Inoue M, "Transport of a tripeptide, Gly-Pro-Hyp, across the porcine intestinal brush-border membrane", J Pept Sci, 13, pp.468~474 (2007)
- Kang MK, "Dietary collagen hydrolysates ameliorate furrowed and parched skin caused by photoaging in hairless mice", Int J Mol Sci, 22, pp.6137~ (2021)
- Kim DU, "Oral intake of low-molecular-weight collagen peptide improves hydration, elasticity, and wrinkling in human skin : a randomized, double-blind, placebo-controlled study", Nutrients, 10, pp.826~ (2018)
- Tak YJ, "Effect of collagen tripeptide and adjusting for climate change on skin hydration in middle-aged women: a randomized, double-blind, placebo-controlled trial", Front Med(Lausanne), 7, pp.608903~ (2021)
- Morikiri Y, "The collagen-derived compound collagen tripeptide induces collagen expression and extends lifespan via a conserved p38 mitogen-activated protein kinase cascade", Biochem Biophys Res Commun, 505, pp.1168~1173 (2018)
- Evans M, "A randomized, triple-blind, placebo-controlled, parallel study to evaluate the efficacy of a freshwater marine collagen on skin wrinkles and elasticity", J Cosmet Dermatol, 20, pp.825~834 (2021)
- Reagan-Shaw S, "Dose translation from animal to human studies revisited", FASEB J, 22, pp.659~661 (2008)
- Proksch E, "Oral supplementation of specific collagen peptides has beneficial effects on human skin physiology : a double-blind, placebo-controlled study", Skin Pharmacol Physiol, 27, pp.47~55 (2014)
- Choi SY, "Effects of collagen tripeptide supplement on skin properties : a prospective, randomized, controlled study", J Cosmet Laser Ther, 16, pp.132~137 (2014)
- Inoue N, "Ingestion of bioactive collagen hydrolysates enhance facial skin moisture and elasticity and reduce facial ageing signs in a randomised double-blind placebo-controlled clinical study", J Sci Food Agric, 96, pp.4077~4081 (2016)
- Chung JH, "Cutaneous photodamage in Koreans : influence of sex, sun exposure, smoking, and skin color", Arch Dermatol, 137, pp.1043~1051 (2001)
- Blume-Peytavi U, "Age-associated skin conditions and diseases : current perspectives and future options", Gerontologist (2016)
- Pérez-Sánchez A, "Nutraceuticals for skin care : a comprehensive review of human clinical studies", Nutrients, 10, pp.403~ (2018)
- Zhang S, "Fighting against skin aging : the way from bench to bedside", Cell Transplant, 27, pp.729~738 (2018)
- Constantin MM, "Skin hydration assessment through modern non-invasive bioengineering technologies", Maedica(Buchar), 9, pp.33~38 (2014)
- Friedman PM, "3D in-vivo optical skin imaging for topographical quantitative assessment of non-ablative laser technology", Dermatol Surg, 28, pp.199~204 (2002)
- Genovese L, "An insight into the changes in skin texture and properties following dietary intervention with a nutricosmeceutical containing a blend of collagen bioactive peptides and antioxidants", Skin Pharmacol Physiol, 30, pp.146~158 (2017)
- Ito N, "Effects of composite supplement containing collagen peptide and ornithine on skin conditions and plasma IGF-1 levels-a randomized, double-blind, placebo-controlled trial", Mar Drugs, 16, pp.482~ (2018)
- Borumand M, "Daily consumption of the collagen supplement Pure Gold Collagen® reduces visible signs of aging", Clin Interv Aging, 9, pp.1747~1758 (2014)
- Sibilla S, "Effects of a nutritional supplement containing collagen peptides on skin elasticity, hydration and wrinkles", J Med Nutr Nutraceut, 4, pp.47~ (2015)
- Proksch E, "Oral intake of specific bioactive collagen peptides reduces skin wrinkles and increases dermal matrix synthesis", Skin Pharmacol Physiol, 27, pp.113~119 (2014)
- Heinrich U, "Antioxidant supplements improve parameters related to skin structure in humans", Skin Pharmacol Physiol, 19, pp.224~231 (2006)
- Poljsak B, "Skin and antioxidants", J Cosmet Laser Ther, 15, pp.107~113 (2013)
- Cho HS, "Anti-wrinkling effects of the mixture of vitamin C, vitamin E, pycnogenol and evening primrose oil, and molecular mechanisms on hairless mouse skin caused by chronic ultraviolet B irradiation", Photodermatol Photoimmunol Photomed, 23, pp.155~162 (2007)
- Hercberg S, "Antioxidant supplementation increases the risk of skin cancers in women but not in men", J Nutr, 137, pp.2098~2105 (2007)
- Shin JH, "The beneficial effects of collagen tripeptide on deep wrinkling and skin moisturization : a randomized controlled trial", J Food Nutr Res, 9, pp.508~515 (2021)
- Draelos ZD, "The science behind skin care : Moisturizers", J Cosmet Dermatol, 17, pp.138~144 (2018)
- Yamamoto S, "Absorption and urinary excretion of peptides after collagen tripeptide ingestion in humans", Biol Pharm Bull, 39, pp.428~434 (2016)
- Shigemura Y, "Effect of prolyl-hydroxyproline(Pro-Hyp), a food-derived collagen peptide in human blood, on growth of fibroblasts from mouse skin", J Agric Food Chem, 57, pp.444~449 (2009)
- Makpol S, "Modulation of collagen synthesis and its gene expression in human skin fibroblasts by tocotrienol-rich fraction", Arch Med Sci, 7, pp.889~895 (2011)
- Turakainen H, "Synthesis of hyaluronic acid and collagen in skin fibroblasts cultured from patients with osteogenesis imperfecta", Biochim Biophys Acta, 628, pp.388~397 (1980)
- Wang Z, "Moisturizing and antioxidant effects of Artemisia argyi essence liquid in HaCaT keratinocytes", Int J Mol Sci, 24, pp.6809~ (2023)
- Ishikawa T, "Measurement of skin elastic properties with a new suction device(I) : relationship to age, sex and the degree of obesity in normal individuals", J Dermatol, 22, pp.713~717 (1995)
- Gosline J, "Elastic proteins : biological roles and mechanical properties", Philos Trans R Soc Lond B Biol Sci, 357, pp.121~132 (2002)
