Attenuation of Diabetic Conditions by Sida rhombifolia in Moderately Diabetic Rats and Inability to Produce Similar Effects in Severely Diabetic in Rats
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Objectives: This study was done out to evaluate theeffects of Sida rhombifolia methanol extract (SRM) ondiabetes in moderately diabetic (MD) and severely diabetic(SD) Sprague-Dawley rats. Methods: SRM was prepared by soaking the powderedplant material in 70% methanol and rota evaporatingthe methanol from the extract. Effective hypoglycemicdoses were established by performing oral glucose tolerancetests (OGTTs) in normal rats. Hourly effects ofSRM on glucose were observed in the MD and the SDrats. Rats were grouped, five rats to a group, into normalcontrol 1 (NC1), MD control 1 (MDC1), MD experimental1 (MDE1), SD control 1 (SDC1), and SD experimental1 (SDE1) groups. All rats in the control groups wereadministered 1 mL of distilled water (DW). The rats inthe MDE1 and the SDE1 groups were administered SRMorally at 200 and 300 mg/kg body weight (BW), respectively,dissolved in 1 mL of DW. Blood was collected initiallyand at intervals of 1 hour for 6 hours to measureblood glucose. A similar experimental design was followedfor the 30-day long-term trial. Finally, rats weresacrificed, and blood was collected to measure bloodglucose, lipid profiles, thiobarbituric acid reactive substances(TBARS) and reduced glutathione (GSH). Results: OGTTs indicated that two doses (200 and300 mg/kg BW) were effective hypoglycemic doses innormal rats. Both doses reduced glucose levels after 1hour in the MDE1 and the SDE1 groups. A long-termtrial of SRM in the MD group showed a reduced glucoselevel, a normal lipid profile, and normal GSH andTBARS levels. In SD rats, SRM had no statistically significanteffects on these parameters. Normal weightwas achieved in the MD rats, but the SD rats showedreduced BW. Conclusion: The study demonstrates that SRM has potentialto alleviate the conditions of moderate diabetic,but not severe diabetes.
Abstract
Objectives: This study was done out to evaluate theeffects of Sida rhombifolia methanol extract (SRM) ondiabetes in moderately diabetic (MD) and severely diabetic(SD) Sprague-Dawley rats. Methods: SRM was prepared by soaking the powderedplant material in 70% methanol and rota evaporatingthe methanol from the extract. Effective hypoglycemicdoses were established by performing oral glucose tolerancetests (OGTTs) in normal rats. Hourly effects ofSRM on glucose were observed in the MD and the SDrats. Rats were grouped, five rats to a group, into normalcontrol 1 (NC1), MD control 1 (MDC1), MD experimental1 (MDE1), SD control 1 (SDC1), and SD experimental1 (SDE1) groups. All rats in the control groups wereadministered 1 mL of distilled water (DW). The rats inthe MDE1 and the SDE1 groups were administered SRMorally at 200 and 300 mg/kg body weight (BW), respectively,dissolved in 1 mL of DW. Blood was collected initiallyand at intervals of 1 hour for 6 hours to measureblood glucose. A similar experimental design was followedfor the 30-day long-term trial. Finally, rats weresacrificed, and blood was collected to measure bloodglucose, lipid profiles, thiobarbituric acid reactive substances(TBARS) and reduced glutathione (GSH). Results: OGTTs indicated that two doses (200 and300 mg/kg BW) were effective hypoglycemic doses innormal rats. Both doses reduced glucose levels after 1hour in the MDE1 and the SDE1 groups. A long-termtrial of SRM in the MD group showed a reduced glucoselevel, a normal lipid profile, and normal GSH andTBARS levels. In SD rats, SRM had no statistically significanteffects on these parameters. Normal weightwas achieved in the MD rats, but the SD rats showedreduced BW. Conclusion: The study demonstrates that SRM has potentialto alleviate the conditions of moderate diabetic,but not severe diabetes.
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