문관과 미성숙 종자로부터 부정아 유도를 통한 재분화
Regeneration of Xanthoceras sorbifolium via Adventitious Shoot Formation from Immature Seeds
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
본 연구는 종자의 높은 유지 함량으로 인해 식･약용 및 바이오에너지 원료 소재로 활용 가치가 높은 문관과(Xanthoceras sorbifolium)의 부정아유도를 통한 기내 재분화 기술을 개발하기 위해 수행되었다. 미성숙 종자 절편을 0.5 mg l-1 6-benzylaminopurine (BA)이 포함된 MS 배지에치상하였을 때 부정아 유도율은 68.6%로 나타났으나, 성숙 종자 절편을 이용하였을 때 상대적으로 매우 낮은 비율(2.86%)로 유도되었다. 부정아유도를 위해 싸이토키닌류의 종류를 달리하여 치상하였을 때 thidazuron (TDZ)과 BA가 포함된 배지에서 70% 이상이 유도되었다. 유도된 부정아의증식을 위해서 절간을 BA와 TDZ이 혼용 처리된 배지에 치상하였을 때 최대 94.29%까지 신초 유도율이 증가되는 것을 확인하였다. 이 후 유도된신초는 정상적인 발근과정을 거쳐 식물체로 재분화되었다. 본 연구에서 제시된 문관과의 부정아 유도 방법을 이용하여 안정적인 종묘확보에 활용될수 있을 것이다.
Abstract
This study was carried out to develop an in vitro regeneration protocol for Xanthoceras sorbifolium, which has high utilization valueas a medicinal or biodiesel raw material owing to the high oil content of its seed. The induction rate of adventitious shoots from cotyledonsof immature zygotic embryos was 68.6% in MS medium with 0.5 mg l-1 benzylaminopurine (BA). In contrast, adventitious shoots wereinduced from mature seeds at a low rate (2.86%). The induction rate of adventitious shoots was more than 70% with thidiazuron (TDZ)and BA treatment. For adventitous shoots multiplication, the shoot induction rate was increased up to 94.29% on 1/2 MS medium supplementedwith combinations of BA and TDZ. The multiplicated shoots were successfully regenerated and showed normal growth and developmentthrough the in vitro root induction stage. These results will be helpful for the development of an efficient regeneration protocol for X. sorbifolium.
참고문헌 (31)
- Annapurna D, "Direct adventitious shoot induction and plant regeneration of Embelia ribes Burm F", Plant Cell, Tissue Organ Cult, 101, pp.269~277 (2010)
- Ao Y, "Analyze of influence factors of seed yield in Xanthoceras sorbifolia Bunge", Chinese Agri. Sci. Bull, 24, pp.300~304 (2008)[DOI]
- Chen Y, "'Yan Xia' : A novel cultivar of Xanthoceras sorbifolium Bunge with ornamental value", HortScience, 56, pp.511~512 (2021)
- Chung KH, "Insertion and expression of GUS gene through in vivo transformation using adventitious bud regeneration technique of dormant scion in populus", Kor. J. Breed, 26, pp.120~128 (1994)
- George EF, "Plant propagation by tissue culture. Part 2", pp.653~669 (1996), Exegetics Limited Publication
- Ghimire BK, "High-frequency direct shoot regeneration from Drymaria cordata wild leaves", Plant Cell, Tissue Organ Cult, 100, pp.209~217 (2010)
- Huettman CA, "Thidiazuron: A potent cytokinin for woody plant tissue culture. Plant cell, cytokinin for woody plant tisssue culture", Plant Cell, Tissue Organ Cult, 33, pp.105~119 (1993)
- Jin CY, "Embryogenic callus induction, plant regeneration and ultrastructure of Xanthoceras sorbifolia Bunge", J. Jilin Agric. Univ, 41, pp.563~568 (2019)
- 김지아, "Eucalyptus pellita의 절편 위치에 따른 부정아유도", 농업생명과학연구, 48(4), pp.125~131 (2014), 농업생명과학연구원[DOI]
- 김용욱, "백합나무 5년생 체세포배 유래 클론배양묘 및 실생묘 간의 생장특성 비교", 한국산림과학회지, 101(4), pp.613~618 (2012), 한국산림과학회
- Kong W, "Extraction of kernel oil from Xanthoceras sorbifolia Bunge by novel solvent", China Oils and Fats, 39, pp.18~22 (2014)
- Li J, "Biodiesel production from yellow horn (Xanthoceras sorbifolia Bunge.) seed oil using ion exchange resin as heterogeneous catalyst", Bioresour. Technol, 108, pp.112~118 (2012)
- Li SL, Jin R, "Economic analysis on the project of biodiesel raw material forestry of Xanthoceras in Heilongjiang province", China For. Econ, 95, pp.20~28 (2009)[DOI]
- 염옥희, "할미 꽃 (Pulsatilla koreana NAKAI) 식물 절편체로부터 부정아 유도에 미치는 cytokinin의 영향 및 식물체 재 분화", Journal of Plant Biotechnology, 36(2), pp.170~173 (2009), 한국식물생명공학회
- Liang F, "Mineral elements for new shoot development in Xanthoceras sorbifolia new shoots", J. Zhejiang Univ. Sci, 35, pp.624~634 (2018)
- Liu Y, "Correction : Transcriptome analysis of yellow horn(Xanthoceras sorbifolia Bunge) : A potential oil-rich seed tree for biodiesel in China", PloS One, 9(1) (2014)
- Lu CY, "The use of thidiazuron in tissue culture", Vitro Cell. Dev. Biol. Plant Biol, 29, pp.92~96 (1993)
- 문흥규, "희귀 및 멸종위기 수종 개느삼의 액아배양을 통한 기내번식", Journal of Plant Biotechnology, 35(3), pp.229~234 (2008), 한국식물생명공학회
- 남상욱, "참나리(Lilium lancifolium Thunb.) 인편으로부터 부정아 발생과 캘러스 유도를 통한 식물체 재생", Journal of Plant Biotechnology, 30(1), pp.53~58 (2003), 한국식물생명공학회
- 오순자, "까마중(Solanum nigrum L.)의 유식물 절편체에서 부정아 형성 및 식물체 재분화", 한국자원식물학회지, 25(2), pp.277~284 (2012), 한국자원식물학회[DOI]
- Park CH, "Micropropagation through adventitous bud formation and direct bulblet production in Dioscorea batatas Decne", Korean J. Plant Tiss. Cult, 23, pp.317~322 (1996)
- Park YS, "Implementation of conifer somatic embryogenesis in clonal forestry: Technical requirements and deployment considerations", Ann. For. Sci, 59, pp.651~656 (2002)
- Ren RY, "Study on effects of different concentrations of TDZ on callus induction of Xanthoceras sorbifolia", Northern Hort, 2011, pp.127~129 (2011)[DOI]
- Shen Z, "Evaluation of biodiesel from Xanthoceras sorbifolia Bunge seed kernel oil from 13 areas in China", J. For Res, 30, pp.869~877 (2019)
- Singh AK, "Adventitious shoot organogenesis and plant regeneration from cotyledons of Dalbergia sissoo Roxb., a timber yielding tree legume", Plant Cell, Tissue Organ Cult, 68, pp.203~209 (2002)
- Wang H, "Adventitious shoot regeneration from hypocotyl slices of mature apricot (Prunus armeniaca L.) seeds: A feasible alternative for apricot genetic engineering", Sci. Hortic, 128, pp.457~464 (2011)
- Wang HY, "Properties of Xanthoceras sorbifolia husk fibers with chemical treatment for applications in polymer composites", J. Mater Sci. Technol, 31, pp.164~170 (2015)
- Wang Y, "Genetic diversity analysis of phenotypic traits among 37 Xanthoceras sorbifolium elite germplasm", J. For. Res, 27, pp.140~147 (2022)
- Wendling I, "Maturation and related aspects in clonal forestry-part Ⅱ : Reinvigoration, rejuvenation and juvenility maintenance", New Forests, 45, pp.473~486 (2014)
- Xiang XQ, "Afforestation technique of Xanthoceras sorbifolia Bunge", Asian Agri. Res, 4, pp.81~84 (2012)
- Yao ZY, "Biodiesel production from Xanthoceras sorbifolia in China : Opportunities and challenges", Renewable Sustainable Energy Rev, 24, pp.57~65 (2013)