High-frequency regeneration of plants in vitro from seedling-derived apical bud explants of Tilia mandshurica Rupr. & Maxim
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This work describe an efficient method for the shoot induction and plant regeneration of seedling-derived apical bud explants of Tilia mandshurica Rupr. & Maxim. The highest rate of shoot induction (82.2%) was obtained when apical bud explants from juvenile seedlings (5 months old) were cultured on Murashige and Skoog (MS) medium containing 1.0 mg/L 6-benzylaminopurine (BAP). However, apical bud explants obtained from mature trees (12 years old) did not produce any shoots, even with BAP supplementation. Among the three cytokinins tested for shoot multiplication (BAP, zeatin, and kinetin), BAP was the most effective; the highest number of shoots per explant (2.1) was observed on MS medium supplemented with 1.0 mg/L BAP. In contrast, the longest average shoot length (3.0 cm) was observed after growth on MS medium with 2.0 mg/L zeatin. No multiplication occurred when apical bud explants were cultured with kinetin-supplemented media. During rooting of in vitro-elongated shoots, the highest rooting rate (100%) was observed in half-strength MS medium supplemented with 0.5 ~ 1.0 mg/L indole-3-butyric acid (IBA) or 3.0 mg/L 1-naphthaleneacetic acid (NAA). During the acclimatization process, plantlets that were rooted on the IBA (0.5 mg/L)- supplemented medium had the highest survival rate (100%) and maximum root length (18.5 cm). These findings suggest that a low concentration (0.5 mg/L) of IBA is appropriate for the rooting and acclimatization of T. mandshurica. Plants were successfully transferred to the greenhouse with a 100% survival rate. This protocol will be useful for the large-scale propagation of Tilia species.
Abstract
This work describe an efficient method for the shoot induction and plant regeneration of seedling-derived apical bud explants of Tilia mandshurica Rupr. & Maxim. The highest rate of shoot induction (82.2%) was obtained when apical bud explants from juvenile seedlings (5 months old) were cultured on Murashige and Skoog (MS) medium containing 1.0 mg/L 6-benzylaminopurine (BAP). However, apical bud explants obtained from mature trees (12 years old) did not produce any shoots, even with BAP supplementation. Among the three cytokinins tested for shoot multiplication (BAP, zeatin, and kinetin), BAP was the most effective; the highest number of shoots per explant (2.1) was observed on MS medium supplemented with 1.0 mg/L BAP. In contrast, the longest average shoot length (3.0 cm) was observed after growth on MS medium with 2.0 mg/L zeatin. No multiplication occurred when apical bud explants were cultured with kinetin-supplemented media. During rooting of in vitro-elongated shoots, the highest rooting rate (100%) was observed in half-strength MS medium supplemented with 0.5 ~ 1.0 mg/L indole-3-butyric acid (IBA) or 3.0 mg/L 1-naphthaleneacetic acid (NAA). During the acclimatization process, plantlets that were rooted on the IBA (0.5 mg/L)- supplemented medium had the highest survival rate (100%) and maximum root length (18.5 cm). These findings suggest that a low concentration (0.5 mg/L) of IBA is appropriate for the rooting and acclimatization of T. mandshurica. Plants were successfully transferred to the greenhouse with a 100% survival rate. This protocol will be useful for the large-scale propagation of Tilia species.
참고문헌 (31)
- Ashraf MF, "Effect of cytokinin types, concentrations and their interactions on in vitro shoot regeneration of Chlorophytum borivilianum Sant. & Fernandez", Electronic Journal of Biotechnology, 17, pp.275~279 (2014)
- Ali A, "Effect of different concentrations of auxins on in vitro rooting of olive cultivar ‘Moraiolo’", Pak. J. Bot., 41(3), pp.1223~1231 (2009)
- Basto S, "Effects of donor plant age and explants on in vitro culture of Cedrela montana Moritz ex Turcz", Univ. Sci., 17(3), pp.263~271 (2012)
- Chalupa V, "Plant regeneration by somatic embryogenesis from cultured immature embryos of oak (Quercus robur L.)and linden (Tilia cordata Mill.)", Plant Cell Rep, 9, pp.398~401 (1990)
- Chae SC, "Influence of auxin concentration on in vitro rooting of Chrysanthemum Morifolium Ramat", Biosci Biotech Res Asia, 3(2) (2016)
- Grzegorczyk-Karolak I, "The effect of cytokinins on shoot proliferation, secondary metabolite production and antioxidant potential in shoot cultures of Scutellaria alpina", Plant Cell Tiss Organ Cult, 122, pp.699~708 (2015)
- Júlia H, "In vitro rooting and acclimatization of Amelanchier alnifolia (Nutt.) Nutt. ex M. Roem: Testing of auxin, spermidine, and gibberellin for overcoming dormancy", Journal of Berry Research, 9(3), pp.549~561 (2019)
- Kim JH, "Genetic Manipulation of Woody Plants", pp.473~474 (1988), Plenum Press
- Tae Dong Kim, "Micropropagation of Tilia amurensis via Repetitive Secondary Somatic Embryogenesis", Journal of Plant Biotechnology, 33(4), pp.243~248 (2006), 한국식물생명공학회
- Kim TD, "Developmental plasticity of glandular trichomes into somatic embryogenesis in Tilia amurensis", Ann Bot, 100(2), pp.177~183 (2007)
- Krupa-Małkiewicz M, "In vitro rooting of Crocosmia × crocosmiiflora ‘Lucifer’", Folia Hort, 29(1), pp.3~10 (2017)
- Lee DK, "Propagation of Tilia species by cutting", Kor J Apiculture, 4, pp.56~60 (1989)
- Ling WT, "Shoot induction from axillary shoot tip explants of fig (Ficus carica) cv. Japanese BTM 6", Tropical Life Sciences Research, 29(2), pp.165~174 (2018)
- Morsink WAG, "Root and shoot development on cuttings of basswood(Tilia Americana)as affected by auxin treatments and size cuttings", Can J For Res, 4, pp.246~249 (1974)
- Morsink WAG, "The effect of some monohydroxybenzoic and dihyroxybenzoic acids as auxin synergists on rooting softwood cuttings of basswood (Tilia Americana)L.) under mist", Can J For Res, 5, pp.500~502 (1975)
- Moon HK, "Somatic embryogenesis and plant regeneration from cultured immature zygotic embryos of Tilia mandshurica Rupr", Res Rep For Gen Res Inst Korea, 32, pp.64~71 (1996)
- 문흥규, "액아배양에 의한 유묘 및 성숙 히어리나무의 기내변식", Journal of Plant Biotechnology, 29(2), pp.117~121 (2002), 한국식물생명공학회
- Murashige T, "A revised medium for rapid growth and bioassays with tobacco tissue cultures", Physiol. Plant, 15, pp.473~497 (1962)
- Northmore JA, "Effects of Media Composition and Auxins on Adventitious Rooting of Bienertia sinuspersici Cuttings", Advances in Bioscience and Biotechnology, 6, pp.629~636 (2015)
- Panchev V, "Seed propagation of Tilia sp. - agrobiological and technological aspects", Agrarni Nauki, 11(25), pp.33~40 (2019)
- Rathwell R, "In vitro propagation of cherry birch (Betula lenta L.)", Can. J. Plant Sci, 96, pp.571~578 (2016)
- Srinidhi HV, "Micropropagation of Adult and Juvenile Neem (Azadirachta indica A. Juss)", Journal of Crop Improvement, 21(4), pp.221~232 (2008)
- Tawfik AA, "Micropropagation of Koelreuteria bipinnata Using Juvenile and Mature Explants", Current Applied Science and Technology, 20(3), pp.470~478 (2020)
- Üçler AÖ, "In vitro Plantlet Regeneration from Mature Embryos of Linden (Tilia platyphyllos Scop.)and Multiplication of its Buds", Turk J Agric For, 25, pp.181~186 (2001)
- Vasudevan A, "Multiple shoot induction from the shoot tip explants of cucumber (Cucumis sativus L.)", Cucurbit Genetics Cooperative Report, 24, pp.8~12 (2002)
- Youn Y, "In vitro plantlet regeneration from axillary buds of Tilia amurensis mature trees and clonal variation in tissue culturability", J Kor For Soc, 79, pp.109~114 (1990)
- Youn Y, "In vitro plantlet regeneration from axillary buds of mature trees of Tilia cordata", J Jpn For Soc, 70, pp.315~317 (1988)
- 윤택승, "용기의 형태와 용적에 따른 찰피나무 유묘의 생장", 생물환경조절학회지, 14(4), pp.238~243 (2005), (사) 한국생물환경조절학회
- Yang Y, "Effects of exponential fertilization on growth and root morphology of Tilia amurensis seedlings", Journal of Nanjing Forestry University (Natural Sciences Edition), 44(2) (2020)
- Yang LX, "Reproduction Techniques for hardwood cutting of Tilia amurensis", Advanced Materials Research, pp.183~185 (2011)
- Zayova E, "Effect of different auxins on in vitro rooting of Paulownia elongata plants", Genetics and Plant Physiology, 4(3-4), pp.155~162 (2014)