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The Effect of Enamel Matrix Derivatives on the Collagen Formation by Human Periodontal Ligament Stem Cells both in vitro and in vivo Analysis

박소연|김동준|박정철|조규성|오상엽|김창성|차재국
대한치과의사협회 (korea dental association)|2015.11|대한치과의사협회지 (Journal of Korean Dental Association)|제53권 제12호|pp. 935-948|DOI 10.22974/jkda.2015.53.12.005

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regenerationperiodontal ligamentenamel matrix proteinsperiodontiumtissue engineeringmesenchymal stromal cells
주제분류치의학

초록

Abstract

목적: 법랑기질 유도체(EMD)가 사람 치주인대 줄기세포(hPDLSC)의 조직 형성능에 미치는 영향을 in vitro와 in vivo 분석 모델을 이용해 평가한다. 재료 및 방법: hPDLSC를 배양하여 운반체와 함께 면역 억제된 쥐 등에 이식하였다; (1)대조군: EMD 처치하지 않은 운반체에 심어진 hPDLSC 군 (EMD-/hPDLSC+), (2)실험군: EMD 처치한 운반체에 심어진 hPDLSC군 (EMD+/hPDLSC+). 각 군당 5마리씩 시행하고 8주 후 희생하였다. 조직학적, 조직계측학적 분석을 통해 형성된 백악질의 면적과 백악세포의 수 그리고 샤피 섬유의 수를 계측하였으며 면역조직화학적 분석을 통해 백악질과 교원질 형성을 평가하였다. 또한 in vitro에서 hPDLSC의 수용성 교원질과 glycosaminoglycan 형성에 대한 EMD의 효과를 분석하였다. 결과: 조직학적 분석에서 교원질성 치주 인대 조직이 실험군에서 현저하게 많이 생성된 것을 관찰할 수 있었다. 형성된 백악질의 면적과 백악세포의 수는 군 간 차이가 없었으나, 새롭게 형성된 샤피 섬유의 수는 실험군에서 대조군보다 유의하게 많았다(p<0.05). 교원질 형성에 대한 면역조직화학적 분석 결과, 실험군에서 I, III형 교원질과 hydroxyproline의 발현이 높았다. 또한 in vitro에서 hPDLSC에 의한 수용성 교원질과glycosaminoglycan 형성이 EMD의 농도에 비례하여 증가하였다 (p<0.05). 결론: EMD는 hPDLSC에 의한 샤피 섬유 및 교원질 생성을 증가시키고, 이는 새로운 백악질의 기능적 부착과 치주조직 재생에 중요한 역할을한다.

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관련 논문AI추천

Enamel Matrix Derivatives가 사람 치주인대 세포의 특이유전자인 PDLs17, PDLs22의 발현에 끼치는 효과국중기, 김병옥, 김종관, 김흥중, 박주철, 장현선, 한근아대한치주과학회 · Journal of Periodontal & Implant Science2004 · 제34권 제2호Effects of enamel matrix derivatives on the proliferation and the release of growth factors of human periodontal ligament cells방은경, 정겨운대한치과보철학회 · 대한치과보철학회지2016 · 제54권 제3호법랑기질 단백질 유도체가 치주인대양세포 및 조골양세포에 미치는 영향고선일, 김동운, 임성빈, 정진형대한치주과학회 · Journal of Periodontal & Implant Science2003 · 제33권 제2호고농도 포도당 환경에서 EMD(Enamel Matrix Derivatives)가 인간 치주인대 세포와 뼈모세포양 세포(MC3T3-E1)에 미치는 영향권용대, 김선욱, 이백수, 주성숙대한구강악안면외과학회 · 대한구강악안면외과학회지2008 · 제34권 제5호법랑기질유도체를 도포한 타이태늄 표면에서 조골세포의 증식 및 분화구영, 류인철, 박상현, 신승윤, 양승민, 이용무, 이인경, 정종평, 최상묵, 한수부대한치주과학회 · Journal of Periodontal & Implant Science2003 · 제33권 제3호Expression of DSPP mRNA During Differentiation of Human Dental Pulp-derived Cells (HDPC) and Transplantation of HDPC Using Alginate Scaffold신제원, 주성숙, 이와이 야스토모, 하야시 히로유키, 쿠마베지, 나카츠카 미치코, 아이카와 후미코대한구강생물학회 · International Journal of Oral Biology2006 · 제31권 제3호

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