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Expression of DSPP mRNA During Differentiation of Human Dental Pulp-derived Cells (HDPC) and Transplantation of HDPC Using Alginate Scaffold

주성숙|신제원|쿠마베지|아이카와 후미코|나카츠카 미치코|하야시 히로유키|이와이 야스토모
대한구강생물학회 (Korean Academy Of Oral Biology)|2006.09|International Journal of Oral Biology|제31권 제3호|pp. 73-79

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참고문헌

주제분류치의학

초록

Abstract

Tissue stem cells are used for the regenerative medicine. In previous study we observed hard tissue formation of human dental pulp-derived cells using alginate scaffold. In this study, we explore the ability to differentiate of the 13th passage cells with glycerol 2-phosphate disodium salt hydrate (-GP) which accelerate calcification. Reverse transcriptase Polymerase Chain Reaction (RT-PCR), transplants using alginate scaffold and histological examination were performed. We observed the expression of DSPP mRNA on day 10 cultured cells with -GP. In conclusion, the 13th passage cells still have an ability to differentiate into odontoblast-like cells and alginate supports the differentia-tion of cultured cells in the transplants.

참고문헌 (22)

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관련 논문AI추천

A Trial of Screening of Genes Involved in Odontoblasts Differentiation from Human Dental Pulp Stem Cells김현진, Yoon-Kyu Park대한구강생물학회 · International Journal of Oral Biology2012 · 제37권 제4호BMP Expression by Human Cementum-Derived Cells in vitro고현정, W.J. Grzesik대한구강생물학회 · International Journal of Oral Biology2005 · 제30권 제3호In vitro characterization of human dental pulp stem cells isolated by three different methods김의성, 박상혁, 이현우, 장지현, 조규민, Hee-Woong Shin, Mo Kwan Kang대한치과보존학회 · Restorative Dentistry and Endodontics2016 · 제41권 제4호Effects of Chitosan on the Differentiation of MDPC-23 Cells김도경, 김흥중, 박종태, Ju-Hyun Park, Kwang-Hee Cho, Sun-Kyoung Yu, Su Young Kim대한구강생물학회 · International Journal of Oral Biology2010 · 제35권 제3호Dental stem cells as a cell source for tissue engineering박봉욱대한구강악안면외과학회 · 대한구강악안면외과학회지2018 · 제44권 제3호The Effect of Enamel Matrix Derivatives on the Collagen Formation by Human Periodontal Ligament Stem Cells both in vitro and in vivo Analysis김동준, 김창성, 박소연, 박정철, 오상엽, 조규성, 차재국대한치과의사협회 · 대한치과의사협회지2015 · 제53권 제12호

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