수분 및 미생물 첨가제가 알팔파 사일리지의발효특성과 사료가치에 미치는 영향
Effects of Moisture Contents and Microbial Additive on Fermentation Characteristics and Feed Values of Alfalfa Silage
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본 연구는 수분함량과 미생물 첨가제가 알팔파 사일리지의 발효특성과 사료가치에 미치는 영향을 알아보기 위해 수행되었다. 알팔파는 개화 10%시기에서 수확되었으며 수분 함량(M60, M50, M40 및 M30)이 60, 50, 40 및 30%일 때 각각 이용하였다. 1500g을 샘플링한 후, 증류수 10mL을첨가한 미첨가구(NAD)와 Lactococcus lactis 와 Pediococcus pentosaceus의 혼합물을 증류수(0.1g/10mL)에 1.5 x 1010cfu/g 농도로 희석한 후접종한 미생물 균주 첨가구(ADD)를 3개월 및 6개월 발효하였다. 수분 및 미생물 첨가제에 따른 연평균 알팔파의 조단백, 중성세제 불용성 섬유및 산성세제 불용성 섬유에서 차이가 없었다(p>0.05). 모든 발효기간에서 pH는 ADD의 M40에서 가장 낮았다(p<0.05). NAD 처리구에서는 젖산이M50에서 가장 높았고(p<0.05), ADD 처리군에서는 M40 시험구에서 젖산이 가장 높았다(p<0.05). NAD와 ADD의 M60은 젖산 함량이 다른 수분함량들에 비해 가장 낮으며(p<0.05) 낙산이 유일하게 검출되었다. 미생물군집의 상대적 풍부도는 ADD 처리구의 M40과 M50에서 Homo LAB(Enterococcus, Lactiplantibacillus, Lacticaseibacillus, Lactococcus, Pediococcus)의 비율이 가장 높았고, Clostridium은 M60에서 가장 높았다.
Abstract
This study was conducted to determine the effects of moisture contents and microbial additives on fermentation characteristics andfeed value of alfalfa silage. Alfalfa was harvested at the 10% flowering stage and wilted to ensile alfalfa forages at 60, 50, 40, and30% moisture content (M60, M50, M40, and M30). After sampling 1500 g, 10mL of distilled water was added to no microbial additives(NAD). and a mix of Lactococcus lactis and Pediococcus pentosaceus was dissolved in distilled water (0.1g/10mL) at an added concentrationof 1.5 × 1010 cfu/g and was inoculated to the microbial additives (ADD). After 3 and 6 months of fermentation, samples were collectedfrom each silage and analyzed for feed value and fermentation characteristics. There was no difference in average annual feed valueby moisture and microbial additives (p>0.05). In all fermentation periods, pH was the lowest in M40 apllied ADD (p<0.05). In theNAD treatment group (p<0.05), lactic acid was highest in M50, whereas in the ADD treatment group, it was the highest in the M40test group (p<0.05). The M60 has the lowest lactic acid content (p<0.05) and only detected butyric acid compared to other moisturecontent. In the relative abundance of the microbiome, Homo LAB (Enterococcus, Lactiplantibacillus, Lacticaseibacillus, Lactococcus,and Pediococcus) had the highest ratio in the M40 and M50, and Clostridium was the highest in the M60 in ADD treatment. Therefore,this study concluded that adding microbial additives at 40% or 50% moisture content was advantageous in alfalfa silage.
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