Effects of Microbial and Organic Additives on Fermentation Quality and Aerobic Stability of Barley Silage
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The goal of this study was to evaluate effects of various microbial and organic additives on chemical compositions, fermentation indices, and aerobic stability of barley silage. Youngyang barley harvested at 31.5% dry matter (DM), and ensiled into 20 L bucket silo for 0, 1, 3, 7, 48, and 100 d in quadruplicates with four additives following: sterile destilled water at 1% of fresh forage (CON); Lactobacillus plantarum at 1.2 x 105 cfu/g of fresh forage (CL); Lactobacillus buchneri at rate of 1.2 x 105 cfu/g fresh forage (LB); Fermented Persimmon Extract at 1% of fresh forage (FPE); and Essential Oil at 1% of fresh forage (EO). On 100 d of ensiling, CL and FPE silages had lower (p<0.05) DM than CON silage. Additionally, FPE silage had higher (p<0.05) crude protein than CON silage. All silages with additives, except EO, had higher (p<0.05) neutral detergent fiber (NDF) than CON silage. Silage treated with CL, LB, and FPE had lower in vitro DM digestibility than CON silage, and silages treated with LB and FPE had higher in vitro NDF digestibility (IVNDFD) on 100 d of ensiling. The PFE silage produced the highest (p<0.05) lactate during ensiling period, while LB silage produced the highest (p<0.05) acetate. All inoculated silages had higher (p<0.05) LAB count than control silage. Only CL silage had higher (p<0.05) yeast count than control silage, while the other silages were not differ compared to CON silage. The aerobic stability was higher (p<0.05) in LB and FPE silages than in CON silage. In conclusion, FPE could be an alternative additive to increase IVNDFD, fermentation indices, and aerobic stability of barley silage.
Abstract
The goal of this study was to evaluate effects of various microbial and organic additives on chemical compositions, fermentation indices, and aerobic stability of barley silage. Youngyang barley harvested at 31.5% dry matter (DM), and ensiled into 20 L bucket silo for 0, 1, 3, 7, 48, and 100 d in quadruplicates with four additives following: sterile destilled water at 1% of fresh forage (CON); Lactobacillus plantarum at 1.2 x 105 cfu/g of fresh forage (CL); Lactobacillus buchneri at rate of 1.2 x 105 cfu/g fresh forage (LB); Fermented Persimmon Extract at 1% of fresh forage (FPE); and Essential Oil at 1% of fresh forage (EO). On 100 d of ensiling, CL and FPE silages had lower (p<0.05) DM than CON silage. Additionally, FPE silage had higher (p<0.05) crude protein than CON silage. All silages with additives, except EO, had higher (p<0.05) neutral detergent fiber (NDF) than CON silage. Silage treated with CL, LB, and FPE had lower in vitro DM digestibility than CON silage, and silages treated with LB and FPE had higher in vitro NDF digestibility (IVNDFD) on 100 d of ensiling. The PFE silage produced the highest (p<0.05) lactate during ensiling period, while LB silage produced the highest (p<0.05) acetate. All inoculated silages had higher (p<0.05) LAB count than control silage. Only CL silage had higher (p<0.05) yeast count than control silage, while the other silages were not differ compared to CON silage. The aerobic stability was higher (p<0.05) in LB and FPE silages than in CON silage. In conclusion, FPE could be an alternative additive to increase IVNDFD, fermentation indices, and aerobic stability of barley silage.
참고문헌 (32)
- Adesogan AT, "The influence of treatment with dual-purpose bacterial inoculants or soluble carbohydrates on the fermentation and aerobic stability of bermudagrass", J. Dairy Sci, 87, pp.3407~3416 (2004)
- Adesogan AT, "Effect of Lactobacillus buchneri, L. fermentum or Leuconostoc mesenteroides inoculants or a chemical additive on the fermentation, aerobic stability and nutritive value of crimped wheat grains", J. Dairy Sci, 86, pp.1789~1796 (2003)
- AOAC, "Official methods of analysis", Association of Official Analytical Chemists, ~ (1995)
- Arriola KG, "Stay-green ranking and maturity of corn hybrids: 1. Effects on dry matter yield, nutritional value, fermentation characteristics, and aerobic stability of silage hybrids in Florida", J. Dairy Sci, 95, pp.964~974 (2012)
- Candan F, "Antioxidant and antimicrobial activity of essential oil and methanol extract of Achillea millefolium subsp. millefolium Afan. (Asteraceae)", J. Ethnopharmacol, 87, pp.215~220 (2003)
- Chaney AL, "Modified reagents for determination of urea and ammonia", Clin. Chem, 8, pp.130~132 (1962)
- Chao SC, "Screening for inhibitory activity of essential oils on selected bacteria, fungi and viruses", J. Essent. Oil Res, 12, pp.639~649 (2000)
- Danner H, "Acetic acid increases stability of silage under aerobic conditions", Appl. Environ. Microbiol, 68, pp.562~567 (2003)
- Filya I, "The effect of Lactobacillus buchneri and Lactobacillus plantarum on the fermentation, aerobic stability, and ruminal degradability of low dry matter corn and sorghum silages", J. Dairy Sci, 86, pp.3575~3581 (2003)
- Hargreaves A, "Effect of storage of growth on the chemical composition, nutritive value and ensilability of whole-crop barley", Anim. Feed. Sci. Technol, 152, pp.50~61 (2009)
- Huisden CM, "Effect of applying molasses or inoculant containing homofermentative and heterofermentative bacteria at two rates on fermentation and aerobic stability of corn silage", J. Dairy Sci, 92, pp.690~697 (2009)
- Kim JM, "Antibacterial activity of some essential oil components against five food borne pathogens", J. Agric. Food. Chem, 43, pp.2839~2845 (1995)
- Kim SB, "Effect of fermented persimmon extract supplements on chemical composition and fermentation characteristic of barley silage", Anim. Sci. J, 84, pp.403~408 (2013)
- Kim SC, "The effects of increasing the level of dietary wormwood (Artemisia montana Pampan) on intake, digestibility, N balance and ruminal fermentation characteristics in sheep", Livest. Sci, 100, pp.261~269 (2005)
- Kittelmann M, "Isolation and classification of acetic acid bacteria from high percentage vinegar fermentations", Appl. Microbiol. Biotechnol, 30, pp.47~52 (1989)
- Kung Jr L, "The effect of treating alfalfa with Lactobacillus buchneri 40788 on silage fermentation, aerobic stability, and nutritive value for lactating dairy cows", J. Dairy Sci, 86, pp.336~343 (2003)
- Kung Jr L, "A blend of essential plant oils used as an additive to alter silage fermentation or used as a feed additive for lactation dairy cows", J. Dairy Sci, 95, pp.1930~1941 (2008)
- 이혁준, "Effects of Bacterial Inoculants and Cutting Height on Fermentation Quality of Barley Silage", 한국초지조사료학회지, 34(3), pp.163~168 (2014), 한국초지조사료학회
- McDonald P, "The biochemistry of silage" (1991), Chalcombe Publ
- Palmqvist E, "Main and interaction effects of acetic acid, furfural, and p-hydroxybenzoic acid on growth and ethanol productivity of yeasts", Biotechnology and Bioengineering, 63, pp.46~55 (1998)
- 박태일, "한국에서 개발된 총체사료용 보리 품종의 형태적 특성", 한국초지조사료학회지, 28(3), pp.193~202 (2008), 한국초지조사료학회
- Ranjit NK, "The effect of Lactobacillus buchneri, Lactobacillus plantarum, or a chemical preservative on the fermentation and aerobic stability of corn silage", J. Dairy Sci, 83, pp.526~536 (2000)
- Sakanaka S, "Comparison of antioxidant properties of persimmon vinegar and some other commercial vinegar in radical-scavenging assays and on liquid oxidation in tuna homogenates", Food Chem, 107, pp.739~744 (2008)
- STAT user’s guide: Version 9, "SAS", pp.2002, SAS Institute Inc
- Souza EL, "Effectiveness of Origanum vulgare L. essential oil to inhibit the growth of food spoiling yeast", Food Control, 18, pp.409~413 (2007)
- Tilley JMA, "A two-stage technique for the in vitro digestion of forage crops", Grass. Forage. Sci, 18, pp.104~111 (1963)
- Trias R, "Lactic acid bacteria from fresh fruit and vegetables as biocontrol agents of phytopathogenic bacteria and fungi", Int. Microbiol, 11, pp.231~236 (2008)
- Van Soest PJ, "Methods for dietary fiber, neutral detergent fiber and non-starch polysaccharides in relation to animal nutrition", J. Dairy Sci, 74, pp.3583~3597 (1991)
- Wannissorn B, "Antibacterial properties of essential oils from Thai medicinal plants", Fitoterapia, 76, pp.233~236 (2005)
- Weinberg ZG, "Effect of lactic acid bacteria inoculants on in vitro digestibility of wheat and corn silages", J. Dairy Sci, 90, pp.4754~4762 (2007)
- 정용진, "식품유해세균에 대한 식초의 향균효과", Food Science and Preservation, 11(1), pp.117~121 (2004), 한국식품저장유통학회
- Woolford MK, "The detrimental effects of air on silage", J. Appl. Bacteriol, 68, pp.101~116 (1990)