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Effects of formic acid and lactic acid bacteria inoculant on main summer crop silages in Korea

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주제분류축산학

초록

To improve the fermentation quality of silage and reduce the nutrients loss of raw materials during the ensiling process, silage additives are widely used. The effect of additives on silage is also affected by the species of crop. Therefore, this study was designed to explore the effects of formic acid (FA) and lactic acid bacterial inoculant on the quality of main summer crop silage. The experiment was consisted on split-plot design with three replications. The experiment used the main summer forage crops of proso millet (“Geumsilchal”), silage corn (“Gwangpyeongok”), and a sorghum-sudangrass hybrid (“Turbo-gold”). Treatments included silage with Lactic acid bacterial Inoculant (Lactobacillus plantarum [LP], 1.0 × 106 CFU/g fresh matter), with FA (98%, 5 mL/kg), and a control (C, without additive). All silages were stored for 60 days after preparation. All additives significantly increased the crude protein content and in vitro dry matter digestibility (IVDMD) of the silages and also reduced the content of ammonia nitrogen (NH3-N) and pH. Corn had the highest content of IVDMD, total digestible nutrients and relative feed value among silages. Compared with the control, irrespective of whether FA or LP was added, the water soluble carbohydrate (WSC) of three crops was largely preserved and the WSC content in the proso millet treated with FA was the highest. The treatment of LP significantly increased the lactic acid content of the all silage, while the use of FA significantly increased the content of acetic acid (p < 0.05). The highest count of lactic acid bacteria (LAB) was detected in the LP treatment of corn. In all FA treatment groups, the total microorganism and mold numbers were significantly lower than those of the control and LP groups (p < 0.05). In conclusion, both additives improved the fermentation quality and nutritional composition of the main summer forage crops. The application of FA effectively inhibited the fermentation of the three crops, whereas LAB promoted fermentation. So, both FA and LP can improve the quality of various species of silage.

Abstract

To improve the fermentation quality of silage and reduce the nutrients loss of raw materials during the ensiling process, silage additives are widely used. The effect of additives on silage is also affected by the species of crop. Therefore, this study was designed to explore the effects of formic acid (FA) and lactic acid bacterial inoculant on the quality of main summer crop silage. The experiment was consisted on split-plot design with three replications. The experiment used the main summer forage crops of proso millet (“Geumsilchal”), silage corn (“Gwangpyeongok”), and a sorghum-sudangrass hybrid (“Turbo-gold”). Treatments included silage with Lactic acid bacterial Inoculant (Lactobacillus plantarum [LP], 1.0 × 106 CFU/g fresh matter), with FA (98%, 5 mL/kg), and a control (C, without additive). All silages were stored for 60 days after preparation. All additives significantly increased the crude protein content and in vitro dry matter digestibility (IVDMD) of the silages and also reduced the content of ammonia nitrogen (NH3-N) and pH. Corn had the highest content of IVDMD, total digestible nutrients and relative feed value among silages. Compared with the control, irrespective of whether FA or LP was added, the water soluble carbohydrate (WSC) of three crops was largely preserved and the WSC content in the proso millet treated with FA was the highest. The treatment of LP significantly increased the lactic acid content of the all silage, while the use of FA significantly increased the content of acetic acid (p < 0.05). The highest count of lactic acid bacteria (LAB) was detected in the LP treatment of corn. In all FA treatment groups, the total microorganism and mold numbers were significantly lower than those of the control and LP groups (p < 0.05). In conclusion, both additives improved the fermentation quality and nutritional composition of the main summer forage crops. The application of FA effectively inhibited the fermentation of the three crops, whereas LAB promoted fermentation. So, both FA and LP can improve the quality of various species of silage.

참고문헌 (42)

  1. Kim DA, "Forage crops : its characteristics and cultivation methods" (1991), Seonjinmunhwasa
  2. Sarrantonio M, "Northeast cover crop handbook" (1994), Rodale Institute
  3. Sorghum-sudangrass hybrids (Green Manure Crops), "Valenzuela H", University of Hawaii at Manoa, ~, pp.Report No.: SA-GM-10 (2020)
  4. K. Anand Kumar, "Genetics of Qualitative Traits in Pearl Millet: A Review", Crop Science, 33(1), pp.1~20 (1993), Wiley[DOI]
  5. 김지혜, "동계 사료작물 보리와 하계 사료작물 수수, 기장 및 피 작부방식에 따른 생육과조사료 생산량 비교", 한국초지조사료학회지, 38(4), pp.286~290 (2018), 한국초지조사료학회[DOI]
  6. McDonald P, "The biochemistry of silage" (1991), Chalcombe
  7. Kung L, "Animal response to silage additives", Proceedings of the Conference on Silage: Field to Feedbunk, North American Conference (1997)
  8. Tao Z, "Screening of alfalfa (Medicago sativa L.) silage agents, mechanism of action and application research", pp.2005
  9. R. F. Wilson, "Formic acid as a silage additive for wet crops of cocksfoot and lucerne", The Journal of Agricultural Science, 80(2), pp.225~231 (1973), Cambridge University Press (CUP)[DOI]
  10. Silveira AC, "Efeito de diferentes tratamentos na digestibilidade in vitro de silagens de capim Napier", Rev Da Soc Bras Zootec, 2, pp.216~226 (1973)
  11. "Jean-Baptiste-André Dumas", Science, 3, pp.750~752 (1884)[DOI]
  12. P.J. Van Soest, "Methods for Dietary Fiber, Neutral Detergent Fiber, and Nonstarch Polysaccharides in Relation to Animal Nutrition", Journal of Dairy Science, 74(10), pp.3583~3597 (1991), American Dairy Science Association[DOI]
  13. E. W. Yemm, "The estimation of carbohydrates in plant extracts by anthrone", Biochemical Journal, 57(3), pp.508~514 (1954), Portland Press Ltd.[DOI]
  14. Holland C, "The pioneer forage manual: a nutritional guide", pp.1~55 (1990), Pioneer Hi-Bred International
  15. J. M. A. Tilley, "A TWO-STAGE TECHNIQUE FOR THE IN VITRO DIGESTION OF FORAGE CROPS", Grass and Forage Science, 18(2), pp.104~111 (1963), Wiley[DOI]
  16. Madigan MT, "Brock biology of microorganisms" (2012), Pearson
  17. Podkowka W, "Modern methods of ensiling" (1978), PWRiL
  18. M. J. Playne, "The buffering constituents of herbage and of silage", Journal of the Science of Food and Agriculture, 17(6), pp.264~268 (1966), Wiley[DOI]
  19. G.A. Broderick, "Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media", Journal of Dairy Science, 63(1), pp.64~75 (1980), American Dairy Science Association[DOI]
  20. Amer S, "Effects of water soluble carbohydrate content on ensiling characteristics, chemical composition and in vitro gas production of forage millet and forage sorghum silages", Anim Feed Sci Technol, 177, pp.23~29 (2012)[DOI]
  21. Henderson N, "Silage additives", Anim Feed Sci Technol, 45, pp.35~56 (1993)[DOI]
  22. Rowghani E, "The effects of a microbial inoculant and formic acid as silage additives on chemical composition, ruminal degradability and nutrient digestibility of corn silage in sheep", Iranian J Vet Res, 10, pp.110~118 (2009)
  23. ZG Weinberg, "Ensiling whole-crop wheat and corn in large containers with Lactobacillus plantarum and Lactobacillus buchneri", Journal of Industrial Microbiology & Biotechnology, 28(1), pp.7~11 (2002), Oxford University Press (OUP)[DOI]
  24. Rooke JA, "The chemical composition and nutritive value of grass silages prepared with no additive or with the application of additives containing either Lactobacillus plantarum or formic acid", Grass Forage Sci, 43, pp.87~95 (1988)[DOI]
  25. Jie Zhao, "Effects of lactic acid bacteria and molasses on fermentation dynamics, structural and nonstructural carbohydrate composition and in vitro ruminal fermentation of rice straw silage", Animal Bioscience, 32(6), pp.783~791 (2019), 아세아·태평양축산학회[DOI]
  26. Chamberlain D, "The effectiveness of the extent of fermentation in the silo on the nutritional value of grass silage for milk production", Proceedings of the 9th Silage Conference (1990)
  27. Baytok E, "The effects of formic acid, molasses and inoculant as silage additives on corn silage composition and ruminal fermentation characteristics in sheep", Turk J Vet Anim Sci, 29, pp.469~474 (2005)
  28. Seare T. Desta, "Ensiling characteristics, structural and nonstructural carbohydrate composition and enzymatic digestibility of Napier grass ensiled with additives", Bioresource Technology, 221, pp.447~454 (2016), Elsevier BV[DOI]
  29. Fİilya İ, "The effects of lactic acid bacterial inoculants on the fermentation, aerobic stability and in situ rumen degradability characteristics of maize and sorghum silages", Turk J Vet Anim Sci, 26, pp.815~823 (2002)
  30. Ping Li, "Effects of chemical additives on the fermentation quality and N distribution of alfalfa silage in south of China", Animal Science Journal, 87(12), pp.1472~1479 (2016), Wiley[DOI]
  31. Q. Zhang, "Effects of different additives on fermentation quality and aerobic stability of Leymus chinensis silage", Grass and Forage Science, 73(2), pp.413~419 (2018), Wiley[DOI]
  32. Meeske R, "The effect of the addition of a lactic acid bacterial inoculant to maize at ensiling on silage composition, silage intake, milk production and milk composition", S Afr J Anim Sci, 32, pp.263~270 (2002)
  33. Kung L, "Silage review: interpretation of chemical, microbial, and organoleptic components of silages", J Dairy Sci, 101, pp.4020~4033 (2018)[DOI]
  34. Francisco E. Contreras-Govea, "Lactobacillus plantarum effects on silage fermentation and in vitro microbial yield", Animal Feed Science and Technology, 179(1-4), pp.61~68 (2013), Elsevier BV[DOI]
  35. Victor L Nsereko, "Effects of peptidase inhibitors and other additives on fermentation and nitrogen distribution in perennial ryegrass silage", Journal of the Science of Food and Agriculture, 79(5), pp.679~686 (1999), Wiley[DOI]
  36. A. T. Adesogan, "The effect of different additives on the fermentation quality, aerobic stability and in vitro digestibility of pea", wheat bi-crop silages containing contrasting pea to wheat ratios, 2002, pp.57(1), 25~32[DOI]
  37. Kennedy SJ, "Comparison of the fermentation quality and nutritive value of sulphuric and formic acid‐treated silages fed to beef cattle", Grass Forage Sci, 45, pp.17~28 (1990)[DOI]
  38. Tyrolová Y, "Effects of biological and chemical additives on fermentation progress in maize silage", Czech J Anim Sci, 62, pp.306~312 (2017)[DOI]
  39. Oladosu Y, "Fermentation quality and additives: a case of rice straw silage", BioMed Res Int, pp.1~14 (2016)[DOI]
  40. Jones BA, "Influence of bacterial inoculant and substrate addition to lucerne ensiled at different dry matter contents", Grass Forage Sci, 47, pp.19~27 (1992)[DOI]
  41. T.C. Da Silva, "The effect of a chemical additive on the fermentation and aerobic stability of high-moisture corn", Journal of Dairy Science, 98(12), pp.8904~8912 (2015), American Dairy Science Association[DOI]
  42. Richard E. Muck, "Silage microbiology and its control through additives", Revista Brasileira de Zootecnia, 39(suppl spe), pp.183~191 (2010), FapUNIFESP (SciELO)[DOI]

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