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다양한 운동강도가 혈장 지질과산화물, 항산화효소 및 염증성 사이토카인에 미치는 영향

Effects of diverse exercise Intensities on levels of MDA, SOD, GPx, and TNF-α in human plasma

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한국운동생리학회|2009.02|운동과학 (Exercise Science)|제18권 제1호|pp. 1-10|DOI 10.15857/ksep.2009.18.1.1

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키워드

MDASODTNF-αGPx운동강도exercise intensity혈장human plasma

초록

본 연구의 목적은 동일한 대상자의 다양한 운동강도에서 생성된 지질과산화물과 그 방어기전으로서 항산화효소 발현은 물론 산화반응으로 유도된 염증성 사이토카인 활성 정도를 알아보는데 있다. 20대 성인남성 12명을 대상으로 최대운동부하검사를 통해 안정시 심박수와 최대심박수를 구한 후, 100% heart rate reserve(HRR)는 물론 HRR의 40%, 60%, 80% 강도의 트레드밀 운동(약 400kcal 소모량)을 모든 연구대상자들이 실시하였다. 안정시를 포함한 4가지 강도의 운동을 실시한 직후, 채혈된 혈액을 분리하여 혈장 MDA, SOD, GPx, 그리고 TNF-α의 활성도를 정량화가 가능한 assay kit을 통해 분석하였다. 운동강도가 높을수록 MDA (p<.05)와 SOD(p<.05)는 각각 60% HRR와 80% HRR까지 감소하였으며 , 이후 100% HRR까지는 증가하는 양상을 보였다. GPx는 운동강도에 따라 지속적으로 증가하였으며, 특히 안정시와 40%HRR에 비해 100% HRR 운동강도에서 유의한 증가 (p<.05)를 보인 반면에 운동강도에 따른 TNF-α의 활성에는 유의한 차이는 보이지 않았다. 결론적으로 MDA, SOD, 그리고 GPx의 활성 변화는 낮은 운동강도에 대한 생리적 적응과 고강도에서 늘어난 산소섭취로 인한 산화적 스트레스의 증가에 기인한 것으로 사료된다.

Abstract

We investigated the effects of diverse exercise intensities on levels of MDA, SOD, GPx, and TNF-α in human plasma. Twelve volunteers participated in the exercise program which consisted of four intensities [40, 60, 80, and 100% of heart rate reserve(HRR)] on the treadmill and resting conditions. To determine whether levels of MDA, SOD, GPx, and TNF-α are dependent on exercise intensity, we used commercial assay kits, respectively using isolated human plasma, collected immediately after exercise. As exercise intensity increases, the levels of MDA(p<.05) and SOD(p<.05) decreased to 60% HRR and 80% HRR respectively, and then continuously increased to 100% HRR. In addition, GPx level increased in proportion to the exercise intensity (p<.05), whereas TNF-α level was not changed significantly. In conclusion, the exercise-induced changes of MDA, SOD, and GPx levels in human plasma appears to be attributed to the physiological adaptation to lower exercise intensity and increased oxidative stress as a consequence of higher oxygen consumption.

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