젊은 성인의 아디포넥틴 유전자 다형(G276T)과 비만, 인슐린 저항성, 심폐체력과의 관계
Association of adiponectin genotype (G276T) with obesity, insulin resistance, and cardiorespiratory fitness in young adults
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초록
아디포넥틴(adiponectin)은 지방조직에서 분비되는 특수한 단백질로서 대사증후군과 제2형 당뇨 유전자가 있는 곳으로 추정되는 위치에 있고, 근육의 포도당 유입을 돕고 지방산을 산화시키는 과정을 통해 인슐린 민감도를 증가시킨다. 혈중 아디포넥틴은 비만이나 만성질환 및 제 2형 당뇨 환자에게서 감소하는 것으로 알려져 있고, 아디포넥틴 유전자 다형성에 대한 연구는 임상적 특징들과 연관성이 있다. 본 연구의 목적은 20대 성인을 대상으로 한국인의 아디포넥틴 유전자 다형성(SNP G276T)을 분석하여 다형에 따른 비만, 인슐린 저항성 및 심폐체력과의 연관성을 알아보고자 한다. 연구 방법은 한국인 20대 대학생 677명(남자 475명, 여자 202명)을 대상으로 Real-Time PCR을 이용하여 SNP G276T를 분석하였으며, 심폐체력을 측정하기 위하여 GXT를 통해 최대산소섭취량을 측정하였다. 또한 신체구성(신체질량지수, 체지방량, 허리둘레)과 혈액변인(공복시 글루코스, 공복시 인슐린, 아디포넥틴)을 표준화된 프로토콜에 따라 측정하였다. SNP G276T의 GG 유전자 다형이 GT, TT 유전자 다형 보다 HOMA-IR(P=.012)과 공복시 인슐린 농도(P=.009)가 높게 나타났다. 한국인의 SNP G276T 유전자 다형은 인슐린 저항성 지표로 의미가 있을 것으로 생각되며, SNP G276T의 위험인자는 G 대립 유전자로 생각된다.
Abstract
Adiponectin, an adipose tissue specific protein, has been localized metabolic syndrome and type 2 diabetes gene, increased insulin senstivity following promotes muscle glucose uptake and fatty acid oxidation. Serum adiponectin concentrations were significantly decreased in subjects with obesity and chronic diseases including type 2 diabetes, study of adiponectin genotype is continuous with clinical characteristic. This study was to investigate whether or not the adiponectin single nucleotide polymorphism(G276T) was associated with obesity, insulin resistance, and cardiorespiratory fitness(CRF) in apparently healthy and young adults. The SNP G276T genotype was assessed in a total of 677 college students(475 men and 202 women) by using the Real-Time Quantitative PCR. CRF was measured as the maximum volume of minute oxygen consumption during a graded treadmill testing. Body composition(body mass index, percent body fat, waist circumference) and blood chemistry(fasting glucose, fasting insulin, adiponectin) were measured by using our standardized protocols. With respect to SNP G276T variants, group analyses showed that those with GG genotype had significantly higher homeostasis model assessment of insulin resistance(HOMA-IR) and fasting insulin than those with GT and TT genotype. The findings of the current study suggest that the adiponectin gene SNP G276T polymorphism may be used as a genetic marker to insulin resistance at least in young adults.
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