Development of the automatic multi-pipette system using CNC Machine
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This study presents the development of an automated multi-pipetting system integrated with a computer numerical control (CNC) machine, designed to address the challenges of manual liquid handling in laboratories. Manual pipetting, a widely used method, suffers from inconsistencies, physical strain, and limited scalability, particularly in high-throughput applications. Previous studies have highlighted these issues, emphasizing the need for automation to improve precision, reduce human error, and handle large volumes efficiently. The proposed system leverages direct displacement pipetting technology, which eliminates the variability associated with air displacement methods, providing improved precision and reduced contamination risks. The system's innovative integration of a CNC machine enables precise three-axis motion control, supporting the automated execution of repetitive liquid handling tasks. Four syringe-based pipettes operate simultaneously, ensuring uniform pressure and volume across channels. A user-friendly graphical interface allows customization of workflows, enhancing flexibility and reducing manual intervention. Experimental validation demonstrated low error rates in both position accuracy (3% on the X-axis, 2.7% on the Y-axis) and liquid handling precision (1.4% average inhalation error). This system offers a cost-effective solution, utilizing affordable components such as syringe pistons and stepper motors, making it accessible to resource-limited laboratories and educational institutions.
Abstract
This study presents the development of an automated multi-pipetting system integrated with a computer numerical control (CNC) machine, designed to address the challenges of manual liquid handling in laboratories. Manual pipetting, a widely used method, suffers from inconsistencies, physical strain, and limited scalability, particularly in high-throughput applications. Previous studies have highlighted these issues, emphasizing the need for automation to improve precision, reduce human error, and handle large volumes efficiently. The proposed system leverages direct displacement pipetting technology, which eliminates the variability associated with air displacement methods, providing improved precision and reduced contamination risks. The system's innovative integration of a CNC machine enables precise three-axis motion control, supporting the automated execution of repetitive liquid handling tasks. Four syringe-based pipettes operate simultaneously, ensuring uniform pressure and volume across channels. A user-friendly graphical interface allows customization of workflows, enhancing flexibility and reducing manual intervention. Experimental validation demonstrated low error rates in both position accuracy (3% on the X-axis, 2.7% on the Y-axis) and liquid handling precision (1.4% average inhalation error). This system offers a cost-effective solution, utilizing affordable components such as syringe pistons and stepper motors, making it accessible to resource-limited laboratories and educational institutions.