전라남도 지역 감 바이로이드의 감염상황 및 무병화 효율 연구
Current occurrence of persimmon viroid and citrus viroid in persimmon in JellaNam-do and testing for viroid inactivation methods
0
조회
0
다운로드
3
인용
0
참고문헌
키워드
초록
It is a serious situation that the farmers' incomehas gradually decreased due to the decline of productivity offruit trees infected with viroids. It has been known thatPersimmon viroid (PVd) and Citrus viroid (CVd) are economicallyimportant viroids that can infected persimmon. In this study,the incidence of CVd and PVd in ‘Fuyu’ persimmon wereidentified as 41% and 34% in JeollaNam-do, respectively. The collected persimmon samples infected by both PVd andCVd were used for testing efficiency of the viroid inactivationmethods. The samples were subjected to single treatment ofthe heat treatment (37°C), cold treatment (4°C), or antiviralagent treatment (Ribavirin), and double treatment of combinationsof the three methods. Viroid inactivation efficiency wasconfirmed through RT-PCR. In the case of the samplessubjected to cold treatment for 4 weeks, the viroid inactivationefficiency was most significantly high as 67% against thesurvival rate of 100%. In addition, in the case of the samplestreated for 2 weeks with the antiviral agents and cold treatment, the viroid inactivation rate was similar to that ofthe cold treatment. In conclusion, the cold treatment showedthe highest viroid inactivation efficiency, and this result willprovide valuable information for production of viroid-freepersimmon.
Abstract
It is a serious situation that the farmers' incomehas gradually decreased due to the decline of productivity offruit trees infected with viroids. It has been known thatPersimmon viroid (PVd) and Citrus viroid (CVd) are economicallyimportant viroids that can infected persimmon. In this study,the incidence of CVd and PVd in ‘Fuyu’ persimmon wereidentified as 41% and 34% in JeollaNam-do, respectively. The collected persimmon samples infected by both PVd andCVd were used for testing efficiency of the viroid inactivationmethods. The samples were subjected to single treatment ofthe heat treatment (37°C), cold treatment (4°C), or antiviralagent treatment (Ribavirin), and double treatment of combinationsof the three methods. Viroid inactivation efficiency wasconfirmed through RT-PCR. In the case of the samplessubjected to cold treatment for 4 weeks, the viroid inactivationefficiency was most significantly high as 67% against thesurvival rate of 100%. In addition, in the case of the samplestreated for 2 weeks with the antiviral agents and cold treatment, the viroid inactivation rate was similar to that ofthe cold treatment. In conclusion, the cold treatment showedthe highest viroid inactivation efficiency, and this result willprovide valuable information for production of viroid-freepersimmon.
참고문헌 (14)
- Campbell AI, "Apple virus inactivation by heat therapy and tip propagation", Nature, 195, pp.520~ (1962)
- El-Dougdoug KA, "Elimination of Hop stunt viroid (HSVd) from infected peach and pear plants using cold therapy and chemotherapy", Aust. J. Basic Appl. Sci, 4, pp.54~60 (2010)
- Feng C, "Production of pathogen-free horticultural crops by cryotherapy of in vitro-grown shoot tips", Methods Mol. Biol, 994, pp.463~482 (2013)
- Gambino G, "A Rapid and effective method for RNA extraction from different tissues of grapevine and other woody plants", Phytochem. Anal, 19, pp.520~525 (2008)
- Hansen A, "Elimination of apple chlorotic leaf spot virus from apple shoot cultures by ribavirin", Plant Dis, 69, pp.134~135 (1985)
- Hollings M, "Disease control through virus-free stock", Annu. Rev. Phytopathol, 3, pp.367~396 (1965)
- Ito T, "Genetic characterization of novel putative rhabdovirus and dsRNA virus from Japanese persimmon", J. Gene. Virol, 94, pp.1917~1921 (2013)
- 김대현, "Apple Scar Skin viroid 발생상황 및 Real-time RT-PCR을 이용한 상대정량 분석", 식물병 연구, 16(3), pp.247~253 (2010), 한국식물병리학회
- 김대현, "열처리와 Shoot-Tip Grafting에 의한 감귤 바이러스 무독묘 생산", Journal of Plant Biotechnology, 32(1), pp.45~50 (2005), 한국식물생명공학회
- 이건섭, "열처리와 경정배양을 이용한 바이러스 무병 사과 생산 시스템", 식물병 연구, 19(4), pp.288~293 (2013), 한국식물병리학회
- Morton JF, "Identification of a new Apscaviroid from Japanese persimmon", Arch. Virol, 153, pp.969~972 (2008)
- Paduch-Cichal E, "A low temperature therapy and meristem-tip culture for eliminating four viroids from infected pPlants", J. Phytopathol, 118, pp.341~346 (1987)
- Paprstein F, "Results of in vitro thermotherapy of apple cultivars", Plant Cell. Tiss. Org, 94, pp.347~352 (2008)
- Wina Dian Savitri, "Elimination of Chrysanthemum stunt viroid (CSVd) from Meristem Tip Culture Combined with Prolonged Cold Treatment", Horticulture, Environment, and Biotechnology, 54(2), pp.177~182 (2013), 한국원예학회[DOI]