CodA 고발현 형질전환 고구마의 산화 및 건조 스트레스 내성 증가
Enhanced drought and oxidative stress tolerance in transgenic sweetpotato expressing a codA gene
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Glycine betaine (GB) is one of the compatiblesolutes that accumulate in the chloroplasts of certain halotolerantplants under salt or cold stress. The codA gene for cholineoxidase, the enzyme that converts choline into GB, has beencloned from a soil bacterium Arthrobacter globiformis. Wegenerated transgenic sweetpotato plants [Ipomoea batatas(L.) Lam] expressing codA gene in chloroplasts under thecontrol of the SWPA2 promoter (referred to as SC plants)and evaluated SC plants under oxidative and drought stresses. SC plants showed enhanced tolerance to methyl viologen(MV)-mediated oxidative stress and drought stress due toinduced expression of codA. At 5 μM of MV treatment, allSC plants showed enhanced tolerance to MV-mediated oxidativestress through maintaining low ion leakage and increasedGB levels compared to wild type plants. When plants weresubjected to drought conditions, SC plants showed enhancedtolerance to drought stress through maintaining high relativewater contents and increased codA expression compared to wild type plants. These results suggest that the SC plantsgenerated in this study will be useful for enhanced biomassproduction on global marginal lands.
Abstract
Glycine betaine (GB) is one of the compatiblesolutes that accumulate in the chloroplasts of certain halotolerantplants under salt or cold stress. The codA gene for cholineoxidase, the enzyme that converts choline into GB, has beencloned from a soil bacterium Arthrobacter globiformis. Wegenerated transgenic sweetpotato plants [Ipomoea batatas(L.) Lam] expressing codA gene in chloroplasts under thecontrol of the SWPA2 promoter (referred to as SC plants)and evaluated SC plants under oxidative and drought stresses. SC plants showed enhanced tolerance to methyl viologen(MV)-mediated oxidative stress and drought stress due toinduced expression of codA. At 5 μM of MV treatment, allSC plants showed enhanced tolerance to MV-mediated oxidativestress through maintaining low ion leakage and increasedGB levels compared to wild type plants. When plants weresubjected to drought conditions, SC plants showed enhancedtolerance to drought stress through maintaining high relativewater contents and increased codA expression compared to wild type plants. These results suggest that the SC plantsgenerated in this study will be useful for enhanced biomassproduction on global marginal lands.
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