열처리와 Shoot-Tip Grafting에 의한 감귤 바이러스 무독묘 생산
Production of Virus-Free Stocks from Virus Plant by the Shoot-Tip Grafting and Heat Treatment
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초록
바이러스에 감염된 감귤나무를 생육상에서 주간 40℃, 야간 30℃로 열처리한 후 발생한 신초를 0.3～0.7 mm로 잘라내어 기내에서 치상한 탱자 (P. trifoliata) 대목에 접목하는 열처리와 경정접목 (shoot-tip grafting)을 병행한 방법으로 무독묘 생산에 성공하였다. 감귤 바이러스 즉, citrus tristeza virus (CTV), satsuma dwarf virus (SDV) and citrus tatter leaf virus (CTLV) 에 대한 무독화율은 각각 75.7%, 100.0%, 82.6%였다. 경정접목 시 접수크기는 0.3 mm 이하일때 바이러스 무독화율이 높았으나 접목 후 활착율은 저하되었다. 열처리 후 경정배양 (shoot-tip culture)을 하여 1개월 후에 활착율을 조사한 결과 Yuzu (C. junos)는 74.6%, 그리고 early satsuma mandarin (Iwasagi)은 13.3%를 나타내었다. 그러나 항바이러스제인 rivabirin을 첨가한 후 무독효과를 보기 위하여 후대배양 중에 온주밀감의 특성상 갈변되어 고사함으로서 무독묘를 생산하지 못했다.
Abstract
Virus-free stocks was produced by the combination of the heat treatment of virus infected plant and shoot-tip grafting (STS). To produce virus-free stocks, the plants infected with citrus viruses were used for virus-free stock production using the modified method of STG in thermotherapy at 40℃ for 16 hours in the light, and at 30℃ for 8 hours of darkness for 4 weeks. Trifoliate orange (P. trifoliata) were used as rootstock seedling for STG. Percentages of virus-free stocks against citrus tristeza virus (CTV), satsuma dwarf virus (SDV) and citrus tatter leaf virus (CTLV) were 75.7%, 100.0%, 82.6% respectively. Shoot tip size for successful STG were as small as possible. Less than 0.3 mm of shoot tips gave the hight efficiency of virus free plants but survival rates were low. And, survival rate after shoot-tip culture was analyzed and the rates were dependant on the cultivars; Yuzu cultivar showed the hight survival rate (74.6%) and early satsuma mandarin (Iwasagi) was 13.3% as the lowest cultivar. But citrus trees were not succeed to grown, turned brown, and died.
참고문헌 (12)
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