The Permeation Characteristics of Water- and Oil-soluble Vitamins Using Franz Diffusion Cell
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This study compared the permeation characteristics of water- and oil-soluble vitamins (nicotinamide and retinoic acid) based on the method presented in OECD guideline 428. The skin was positioned between the donor and receptor chambers of Franz diffusion cells. The time intervals for sample collection were 1, 2, 4, 8, and 24 hours, and the collected samples were stored at –80°C prior to analysis. The amount of permeated substances was measured by HPLC.The permeation amount increased in a concentration-dependent manner for the test substances. Substances with high concentrations showed high permeation rates at the initial stage. Nicotinamide, a water-soluble vitamin B, showed higher permeability, flux, and Kp values compared to retinoic acid (an oil-soluble vitamin A). According to the Marzulli definitions, retinoic acid showed permeation rates ranging from ‘very slow’ to ‘moderate,’ while nicotinamide showed rates ranging from ‘slow’ to ‘fast.’ Based on these results, it is expected that this study will contribute to future research on the permeation characteristics of water-soluble and oil-soluble vitamins.
Abstract
This study compared the permeation characteristics of water- and oil-soluble vitamins (nicotinamide and retinoic acid) based on the method presented in OECD guideline 428. The skin was positioned between the donor and receptor chambers of Franz diffusion cells. The time intervals for sample collection were 1, 2, 4, 8, and 24 hours, and the collected samples were stored at –80°C prior to analysis. The amount of permeated substances was measured by HPLC.The permeation amount increased in a concentration-dependent manner for the test substances. Substances with high concentrations showed high permeation rates at the initial stage. Nicotinamide, a water-soluble vitamin B, showed higher permeability, flux, and Kp values compared to retinoic acid (an oil-soluble vitamin A). According to the Marzulli definitions, retinoic acid showed permeation rates ranging from ‘very slow’ to ‘moderate,’ while nicotinamide showed rates ranging from ‘slow’ to ‘fast.’ Based on these results, it is expected that this study will contribute to future research on the permeation characteristics of water-soluble and oil-soluble vitamins.
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