피부흡수 대체시험법의 조건설정을 통한 수용성, 지용성 물질의 투과 특성 연구
A Permeation Characteristics Study of Water- or Oil-soluble Substances through Condition Setting for the In Vitro Skin Absorption Method
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Objectives: The purpose of this study was to compare permeation characteristics in three skin types using oil-soluble benzoic acid and water-soluble caffeine after method condition optimization based on OECD guideline 428. Methods: A Franz diffusion cell, a reliable alternative method for skin permeation, was used. One-milliliter samples were taken and immediately replaced with fresh solution in the receptor chamber at regular time intervals (1, 2, 4, 7, 10 and 24 hr). The amount of test substances was measured by LC-MS/MS. Results: The permeation rate increased dose-dependently, and the permeation orders were KeraSkin > hairless TMmouse full skin > human cadaver epidermis for skin types, and benzoic acid solution > caffeine solution > benzoic acid cream > caffeine cream for type of test materials. Conclusion: According to the definitions of Marzulli, benzoic acid and caffeine would be classified as ‘fast’ and ‘moderate’ compared with the permeation of other chemical species. The setting conditions and permeation characteristics performed in this study are expected to contribute to future permeation studies.
Abstract
Objectives: The purpose of this study was to compare permeation characteristics in three skin types using oil-soluble benzoic acid and water-soluble caffeine after method condition optimization based on OECD guideline 428. Methods: A Franz diffusion cell, a reliable alternative method for skin permeation, was used. One-milliliter samples were taken and immediately replaced with fresh solution in the receptor chamber at regular time intervals (1, 2, 4, 7, 10 and 24 hr). The amount of test substances was measured by LC-MS/MS. Results: The permeation rate increased dose-dependently, and the permeation orders were KeraSkin > hairless TMmouse full skin > human cadaver epidermis for skin types, and benzoic acid solution > caffeine solution > benzoic acid cream > caffeine cream for type of test materials. Conclusion: According to the definitions of Marzulli, benzoic acid and caffeine would be classified as ‘fast’ and ‘moderate’ compared with the permeation of other chemical species. The setting conditions and permeation characteristics performed in this study are expected to contribute to future permeation studies.
참고문헌 (24)
- Simon GA, "The pig as an experimental animal model of percutaneous permeation in man: qualitative and quantitative observations-an overview", Skin Pharmacol Appl Skin Physiol, 13(5), pp.229~234 (2000)
- Elias PM, "Fitzpatrick’s dermatology in general medicine" (2003), McGraw-Hill
- Rogers J, "Stratum corneum lipids: the effect of ageing and the seasons", Arch Dermatol Res, 288, pp.765~770 (1996)
- Fisher GJ, "Mechanisms of photoaging and chronological skin aging", Arch Dermatol, 138, pp.1462~1470 (2002)
- Moser K, "Passive skin penetration enhancement and its quantification in vitro", Eur J Pharm Biopharm, 52, pp.103~112 (2001)
- Ackermann K, "The Phenion full-thickness skin model for percutaneous absorption testing", Skin Pharmacol Physiol, 23, pp.105~112 (2010)
- Brain KR, "Percutaneous penetration of diethanolamine through human skin in vitro: application from cosmetic vehicles", Food Chem Toxicol, 43, pp.681~690 (2005)
- Mickelsen RL, "Evaluation of a simple weight-loss method for determining the permeation of organic liquids through rubber films", Am Ind Hyg Assoc J, 52, pp.445~447 (2005)
- Riviere JE, "Predicting skin permeability from complex chemical mixtures", Toxicol Appl Pharmacol, 208, pp.99~110 (2005)
- Scientific Committee on Cosmetic Products and Non-Food Products, "Opinion concerning Basic criteria for the in vitro assessment of dermal absorption of cosmetic ingredients", SCCNFP, 03, pp.2003, ~
- Reichling J, "In vitro studies on release and human skin permeation of Australian tea tree oil (TTO) from topical formulations", Eur J Pharm Biopharm, 64, pp.222~228 (2006)
- Pappinen S, "Rat epidermal keratinocyte organotypic culture (ROC) compared to human cadaver skin: The effect of skin permeation enhancers", Eur J Pharm Sci, 30, pp.240~250 (2007)
- Rohr UD, "Prodrug of gestodene for matrix-type transdermal drug delivery system", Pharm Res, 15, pp.1419~1424 (1998)
- OECD, "OECD guideline for the testing of chemicals", Skin absorption: in vitromethod, ~ (2004)
- methylphosphonothioate) in the domestic white pig, "Hum Exp Toxicol", 2005, 347~352
- Nielsen JB, "Storage conditions of skin affect tissue structure and subsequent in vitro percutaneous penetration", Skin Pharmacol Physiol, 24, pp.93~102 (2011)
- Potard G, "Quantitative HPLC analysis of sunscreens and caffeine during in vitro percutaneous penetration studies", Int J Pharm, 189, pp.249~260 (1999)
- van de Sandt JJ, "In vitro predictions of skin absorption of caffeine, testosterone, and benzoic acid: a multi-centre comparison study", Regul Toxicol Pharmacol, 39(3), pp.271~281 (2004)
- Guth K, "Suitability of skin integrity tests for dermal absorption studies in vitro", Toxicol In Vitro, 29(1), pp.113~123 (2015)
- Franz TJ, "On the relevance of in vitro data", J Invest Dermatol, 93, pp.633~640 (1975)
- Levintova Y, "An improved in vitro method for measuring skin permeability that controls excess hydration of skin using modified Franz diffusion cells", Int J Pharm, 419, pp.96~106 (2011)
- Barber ED, "The percutaneous absorption of hydroquinone (HQ) through rat and human skin in vitro", Toxicol Lett, 80, pp.167~172 (1995)
- Yamashita F, "Analysis of skin penetration enhancement based on a two-layer skin diffusion model with polar and nonpolar routes in the stratum corneum: dose-dependent effect of 1-geranylazacycloheptan-2-one on drugs with different lipophilicities", Biol Pharm Bull, 16(7), pp.690~697 (1993)
- Wilkinson SC, "Interactions of skin thickness and physicochemical properties of test compounds in percutaneous penetration studies", Int Arch Occup Environ Health, 79, pp.405~413 (2006)