본문 바로가기
KERD
논문KCI 등재

滋陰降火湯이 Propylthiouracil(PTU)로 유발된 Rat의 갑상선기능저하증에 미치는 영향

Effects of Jaeumkanghwa-tang on the Rat Hypothyroidism Induced by Propylthiouracil (PTU)

김동철|김석중
대한한방부인과학회 (The Society Of Oriental Obstetrics & Gynecology)|2014.02|대한한방부인과학회지 (The Journal of Oriental Obstetrics ＆ Gynecology)|제27권 제1호|pp. 41-64|DOI http://dx.doi.org/10.15204/jkobgy2014.27.1.041

2

조회

0

다운로드

4

인용

0

참고문헌

키워드

HypothyroidismLevothyroxinePropylthiouracilJaeumkanghwa-tang
주제분류한의학

초록

Objectives: This study was to evaluate the effect of Jaeumkanghwa-tang (JEKHT)on the propylthiouracil (PTU)-induced rat hypothyroidism. Methods: Six groups, each of 8 rats per group were used in the present study- intact vehicle control, PTU control, Levothyroxine (LT4), JEKHT 500, 250 and125 mg/kg treated groups. JEKHT were administered once a day for 42 days asan oral dose of 500, 250 and 125 mg/kg, and hypothyroidism was induced by dailysubcutaneous treatment of PTU 10 mg/kg for 28 days. The changes on the bodyand organ weight, serum hormone and lipid profiles, liver and testis antioxidantdefense factors were observed with histopathology of organs. Results were comparedwith LT4 0.5 mg/kg intraperitoneally treated rats in this experiment. Results: PTU treatment, marked decrease of body weight, increases of thyroidweight, decreases of liver, testis, epididymis and prostate weights, decreases ofserum Tri-iodothyronine (T3), and Thyroxine (T4) level with increase of serumThyroid-stimulating hormone (TSH) level, decreases of serum testosterone anddihydrotestosterone (DHT) level with increases of serum Follicular stimulatinghormone (FSH) level, increases of serum High density lipoprotein (HDL), decreaseof triglyceride content, increase of serum Aspartate aminotransferase (AST) level,decreases of liver and testis antioxidant defense factors were observed. In addition,marked hyperplasia of follicular cells with decreases of follicular colloid contentsand diameters was additionally demonstrated with the decrease of hepatocyte numbersper unit area due to hypertrophy of hepatocytes related to lipid droplet depositions,increase of a/oligospermatic epididymal tubules with epididymal atrophic changes,seminiferous tubular atrophy with decrease of stage I~Ⅱ seminiferous tubules intestis, prostate tubular atrophic changes at histopathological inspections. However,these PTU induced hypothyroidism and related hepatic and male reproductive organdamages were favorably and dose-dependently inhibited by treatment of JEKHT500, 250 and 125 mg/kg, and JEKHT also effectively regulated the PTU-inducedabnormal antioxidant defense factor changes in the both liver and testis. Conclusions: JEKHT 500, 250 and 125 mg/kg dose-dependently inhibited PTUinducedhypothyroidism and related liver and male reproductive organ damages in rats.

Abstract

Objectives: This study was to evaluate the effect of Jaeumkanghwa-tang (JEKHT)on the propylthiouracil (PTU)-induced rat hypothyroidism. Methods: Six groups, each of 8 rats per group were used in the present study- intact vehicle control, PTU control, Levothyroxine (LT4), JEKHT 500, 250 and125 mg/kg treated groups. JEKHT were administered once a day for 42 days asan oral dose of 500, 250 and 125 mg/kg, and hypothyroidism was induced by dailysubcutaneous treatment of PTU 10 mg/kg for 28 days. The changes on the bodyand organ weight, serum hormone and lipid profiles, liver and testis antioxidantdefense factors were observed with histopathology of organs. Results were comparedwith LT4 0.5 mg/kg intraperitoneally treated rats in this experiment. Results: PTU treatment, marked decrease of body weight, increases of thyroidweight, decreases of liver, testis, epididymis and prostate weights, decreases ofserum Tri-iodothyronine (T3), and Thyroxine (T4) level with increase of serumThyroid-stimulating hormone (TSH) level, decreases of serum testosterone anddihydrotestosterone (DHT) level with increases of serum Follicular stimulatinghormone (FSH) level, increases of serum High density lipoprotein (HDL), decreaseof triglyceride content, increase of serum Aspartate aminotransferase (AST) level,decreases of liver and testis antioxidant defense factors were observed. In addition,marked hyperplasia of follicular cells with decreases of follicular colloid contentsand diameters was additionally demonstrated with the decrease of hepatocyte numbersper unit area due to hypertrophy of hepatocytes related to lipid droplet depositions,increase of a/oligospermatic epididymal tubules with epididymal atrophic changes,seminiferous tubular atrophy with decrease of stage I~Ⅱ seminiferous tubules intestis, prostate tubular atrophic changes at histopathological inspections. However,these PTU induced hypothyroidism and related hepatic and male reproductive organdamages were favorably and dose-dependently inhibited by treatment of JEKHT500, 250 and 125 mg/kg, and JEKHT also effectively regulated the PTU-inducedabnormal antioxidant defense factor changes in the both liver and testis. Conclusions: JEKHT 500, 250 and 125 mg/kg dose-dependently inhibited PTUinducedhypothyroidism and related liver and male reproductive organ damages in rats.

참고문헌 (48)

  1. 조보연, "임상갑상선학", pp.437~476 (2010), 고려의학
  2. 구병삼, "임상 부인과 내분비학", pp.188~ (2001), 고려의학
  3. Wiersinga WM, "Thyroid hormone replacement therapy", Horm Res, 56, pp.74~81 (2001)
  4. 杜鎬京, "東醫腎系學", pp.729~ (1993), 東洋醫學硏究院
  5. 최인구, "眞武湯이 백서의 갑상선기능저하증에 미치는 영향", 대한한방내과학회지, 27(4), pp.879~887 (2006), 대한한방내과학회
  6. 조충식, "當歸四逆湯이 백서의 갑상선기능저하증에 미치는 영향", 대한본초학회지, 22(1), pp.95~102 (2007), 대한본초학회
  7. 조충식, "大營煎이 흰쥐의 갑상선기능저하증에 미치는 영향", 대한본초학회지, 22(4), pp.35~43 (2007), 대한본초학회
  8. 홍민진, "淫羊藿이 실험적으로 유발된 갑상선 기능저하증 동물모델에 미치는 영향과 안전성", Journal of Pharmacopuncture, 14(4), pp.13~22 (2011), 대한약침학회
  9. 이승진, "육종용이 PTU로 유발된 갑상선 기능저하증 동물모델에 미치는 영향", 동의생리병리학회지, 25(6), pp.989~995 (2011), 대한동의병리학회
  10. 김승모, "시호가 PTU (6-n-propyl-2-thiouracil)로 유발된 Rat 갑상샘 기능저하증에 미치는 영향", 동의생리병리학회지, 26(5), pp.714~723 (2012), 대한동의병리학회
  11. 김용석, "人蔘水鍼이 白鼠의 甲狀腺機能低下에 미치는 影響", 경희의학, 6(2), pp.202~210 (1990)
  12. 梁熙台, "鹿茸水鍼이 白鼠의 甲狀腺機能低下에 미치는 影響", 針灸學會誌, 9(1), pp.215~227 (1992)
  13. 龔延賢, "萬病回春", pp.204~205 (1975), 杏林書院
  14. 蘭秀萍, "自擬滋陰降火湯治療更年期不寐療效觀察", 山西職工醫學院學報, 13(3), pp.25~ (2003)
  15. 김유경, "자음강화탕의 알레르기성 영증반응억제 효과", 대한한방내과학회지, 25(2), pp.174~182 (2004), 대한한방내과학회
  16. 정홍매, "자음강화탕 투여 후 상열감의 호전을 보인 타목시펜 복용 중인 유방암 환자 1례", 대한한방내과학회지, 31(2), pp.395~400 (2010), 대한한방내과학회
  17. Mogulkoç R, "Influence of hypovolemic and hypertonic treatments on plasma vasopressin levels and fluid balance in the propylthiouracil -induced hypothyroid rat", Neuro Endocrinol Lett, 20, pp.311~314 (1999)
  18. O'Connor JC, "Evaluation of a 15-day screening assay using intact male rats for identifying steroid biosynthesis inhibitors and thyroid modulators", Toxicol Sci, 69, pp.79~91 (2002)
  19. Kavutcu M, "Reduced enzymatic antioxidant defense mechanism in kidney tissues from gentamicin-treated guinea pigs: effects of vitamins E and C", Nephron, 72, pp.269~274 (1996)
  20. Lowry OH, "Protein measurement with the Folin phenol reagent", J Biol Chem, 193, pp.265~275 (1951)
  21. Jamall IS, "Effects of cadmium on glutathione peroxidase, superoxidase dismutase and lipid peroxidation in the rat heart: a possible mechanism of cadmium cardiotoxicity", Toxicol Appl Pharmacol, 80, pp.33~42 (1985)
  22. Pick E, "Superoxide anion and hydrogen peroxide production by chemically elicited peritoneal macrophages-induction by multiple nonphagocytic stimuli", Cell Immunol, 59, pp.301~318 (1981)
  23. Has UB, "Methods of enzymatic analysis", pp.673~686 (1974), Academic Press Inc
  24. Nishikimi M, "The occurrence of superoxide anion in the reaction of reduced PMS and molecular oxygen", Biochem Biophys Res Comm, 46, pp.849~854 (1972)
  25. Kakkar P, "Modified spectrophotometric assay of SOD", Indian J Biochem Biophys, 2, pp.130~132 (1984)
  26. Subudhi U, "Supplementation of curcumin and vitamin e enhances oxidative stress, but restores hepatic histoarchitecture in hypothyroid rats", Life Sci, 84, pp.372~379 (2009)
  27. Gartner LP, "Color textbook of histology", pp.489~510 (2007), Saunders-Elsevier
  28. Devdhar M, "Hypothyroidism", Endocrinol Metab Clin North Am, 36, pp.595~615 (2007)
  29. Surks MI, "Subclinical thyroid disease: scientific review and guidelines for diagnosis and management", JAMA, 291, pp.228~238 (2004)
  30. Canaris GJ, "Colorado thyroid disease prevalence study", Arch Intern Med, 160, pp.526~534 (2000)
  31. Guyton AC, "Textbook of medical physiology", pp.906~908 (1986), WB Saunder’s Company
  32. 박규열, "억간산이 PTU(6-n-propyl-2-thiouracil)로 유발된 Rat 갑상샘 기능저하증에 미치는 영향", 대구한의대학교 대학원 논문집 (2011)
  33. 金容晟, "加減大補湯이 白鼠의 甲狀腺機能低下症에 미치는 影響", 大韓韓方內科學會誌, 15(2), pp.305~312 (1994)
  34. 閔庚勳, "八味地黃湯이 thiouracil로 誘發한 白鼠의 甲狀腺機能低下에 미치는 影響", 大田大韓醫學硏究所 論文集, 5(1), pp.233~250 (1996)
  35. 임범수, "右歸飮이 白鼠의 甲狀腺機能低下症에 미치는 영향", 대한한의학회지, 21(4), pp.26~36 (2000)
  36. 이정원, "당귀사역가오수유생강탕이 백서의 갑상선기능저하증에 미치는 영향", 석사, 내과학, pp.2001
  37. Yang Y, "Regulated hypothermia in the hypothyroid rat induced by administration of propylthiouracil", Am J Physiol, 272, pp.R1390~R1395 (1997)
  38. Oner J, "Effect of vitamin E on follicular cell proliferation and expression of apoptosis- associated factors in rats with 6-N-propyl-2-thiouracil-induced goitrogenesis", Folia Histochem Cytobiol, 41, pp.213~217 (2003)
  39. Simon-Giavarotti KA, "Enhancement of lindane-induced liver oxidative stress and hepatotoxicity by thyroid hormone is reduced by gadolinium chloride", Free Radic Res, 36, pp.1033~1039 (2002)
  40. Chattopadhyay S, "Differential expression profiles of antioxidant enzymes and glutathione redox status in hyperthyroid rats: a temporal analysis", Comp Biochem Physiol C Toxicol Pharmacol, 146, pp.383~391 (2007)
  41. Dory L, "Rat plasma lipoproteins and apolipoproteins in experimental hypothyroidism", J Lipid Res, 22, pp.287~296 (1981)
  42. Teixeira PDF, "Lipid profile in different degrees of hypothyroidism and effects of levothyroxine replacement in mild thyroid failure", Transl Res, 151, pp.224~231 (2008)
  43. Sahoo DK, "Hypothyroidism impairs antioxidant defence system and testicular physiology during development and maturation", Gen Comp Endocrinol, 156, pp.63~70 (2008)
  44. Nikoobakht MR, "The role of hypothyroidism in male infertility and erectile dysfunction", Urology Journal, 9(1), pp.405~409 (2012)
  45. 김영곤, "항산화제", pp.154~ (2004), 여문각
  46. Sanocka D, "Oxidative stress and male infertility", J Androl, 17, pp.449~454 (1996)
  47. Mutvei A, "The response of individual polypeptides of the mammalian respiratory chain to thyroid hormone", Arch Biochem Biophy, 268, pp.215~220 (1989)
  48. Sahoo DK, "Protective effects of vitamin E and curcumin on L-thyroxine-induced rat testicular oxidative stress", Chem Biol Interact, 176, pp.121~128 (2008)

관련 논문AI추천

益氣補血湯 물 추출물이 PTU로 유발된 Rat의 갑상선기능저하증에 미치는 영향김동철, 손지혜대한한방부인과학회 · 대한한방부인과학회지2015 · 제28권 제3호人蔘養榮湯이 PTU로 유발된 Rat의 갑상선기능저하증에 미치는 영향김동철, 박은영대한한방부인과학회 · 대한한방부인과학회지2015 · 제28권 제2호香砂養胃湯이 PTU로 유발된 Rat의 갑상선기능저하와 간손상에 미치는 영향김승모, 주정현, 최홍식한의병리학회 · 동의생리병리학회지2015 · 제29권 제5호Levothyroxine으로 유발된 갑상선기능항진증 랫트에 대한 양격산화탕, 팔물군자탕 및 청폐사간탕의 효능 비교연구김성태, 최애련사상체질면역의학회 · 사상체질면역의학회지2016 · 제28권 제2호淫羊藿이 실험적으로 유발된 갑상선 기능저하증 동물모델에 미치는 영향과 안전성안세영, 안영민, 이병철, 홍민진대한약침학회 · Journal of Pharmacopuncture2011 · 제14권 제4호금은화가 6-n-propyl-2-thiouracil (PTU) 로 유발된 흰쥐의 갑상샘 기능저하증에 미치는 영향구세광, 이영준한의병리학회 · 동의생리병리학회지2010 · 제24권 제4호

이 간행물의 다른 논문 (14건)

梔子가 대식세포의 Cytokine에 미치는 영향임은미, 차지혜pp. 1-16虎杖根의 파골세포 분화 억제 효과와 기전 연구이창훈, 황덕상, 이경섭, 장희재, 이진무, 장준복pp. 17-27소복축어탕의 마우스 경구 단회투여 독성 평가김동철pp. 28-40加減攝營煎의 Gardnerella vaginalis에 대한 시험관내 항균력 및 Clindamycin과의 병용효과오자영, 김동철pp. 65-80일부 殺蟲解毒類 한약의 Staphylococcus aureus에 대한 시험관 내 항균 및 항염 효과장세란, 김동철pp. 81-100
원문 보기