본문 바로가기
KERD
논문KCI 등재

금은화가 6-n-propyl-2-thiouracil (PTU) 로 유발된 흰쥐의 갑상샘 기능저하증에 미치는 영향

Effects of Lonicerae Flos on the 6-n-propyl-2-thiouracil (PTU)-induced Rat Hypothyroidism

2

조회

0

다운로드

5

인용

0

참고문헌

키워드

주제분류한의학

초록

The object of this study was to evaluate the effect of Lonicerae Flos, aqueous extracts of the dried flower bud part of Lonicera japonica on the 6-n-propyl-2-thiouracil (PTU)-induced rat hypothyroidism. Aqueous extracts of Lonicerae Flos (LF yield = 23.80%) were administered, once day for 42 days from 2 weeks before start of PTU treatment as an oral dose of 500 and 250 mg/kg (body weight), and hypothyroidism was induced by daily subcutaneous treatment of PTU 10 mg/kg for 28 days. The changes on the body weight, thyroid gland weights, serum thyroid hormone – thyroid stimulating hormone (TSH), tri-iodothyronine (T3) and thyroxine (T4), serum lipid profiles – total cholesterol, low density lipoprotein (LDL), high density lipoprotein (HDL) and triglyceride were observedwith liver antioxidant defense system – lipid peroxidation, H2O2, superoxide dismutase (SOD) and catalase (CAT) and serum asparte aminotransferase (AST) and alanine aminotransferase (ALT) analysis. Results were compared with Levothyroxine (LT4) 0.5 mg/kg treated rats. As results of PTU treatment, marked decreases of body weights, serum thyroid hormone levels and triglyceride contents, liver H2O2 and SOD activities were observed with increases of serum AST and HDL contents, liver CAT activities, thyroid gland weight. However, these PTU induced hypothyroidism were dose-dependently inhibited by treatment of LF extracts, and LF extracts effectively regulated the hypothyroidism related changes on the antioxidant defense system. The results obtained in this study suggest that LF extracts have favorable effects on the thyroid hormone productions with beneficial effects on the hypothyroidism mediated by the modulatory effects on the antioxidant defense system.

Abstract

The object of this study was to evaluate the effect of Lonicerae Flos, aqueous extracts of the dried flower bud part of Lonicera japonica on the 6-n-propyl-2-thiouracil (PTU)-induced rat hypothyroidism. Aqueous extracts of Lonicerae Flos (LF yield = 23.80%) were administered, once day for 42 days from 2 weeks before start of PTU treatment as an oral dose of 500 and 250 mg/kg (body weight), and hypothyroidism was induced by daily subcutaneous treatment of PTU 10 mg/kg for 28 days. The changes on the body weight, thyroid gland weights, serum thyroid hormone – thyroid stimulating hormone (TSH), tri-iodothyronine (T3) and thyroxine (T4), serum lipid profiles – total cholesterol, low density lipoprotein (LDL), high density lipoprotein (HDL) and triglyceride were observedwith liver antioxidant defense system – lipid peroxidation, H2O2, superoxide dismutase (SOD) and catalase (CAT) and serum asparte aminotransferase (AST) and alanine aminotransferase (ALT) analysis. Results were compared with Levothyroxine (LT4) 0.5 mg/kg treated rats. As results of PTU treatment, marked decreases of body weights, serum thyroid hormone levels and triglyceride contents, liver H2O2 and SOD activities were observed with increases of serum AST and HDL contents, liver CAT activities, thyroid gland weight. However, these PTU induced hypothyroidism were dose-dependently inhibited by treatment of LF extracts, and LF extracts effectively regulated the hypothyroidism related changes on the antioxidant defense system. The results obtained in this study suggest that LF extracts have favorable effects on the thyroid hormone productions with beneficial effects on the hypothyroidism mediated by the modulatory effects on the antioxidant defense system.

참고문헌 (39)

  1. 조보연, "임상갑상선학. 2판", pp.409~441 (2001), 고려의학
  2. 송영기, "갑상선학", pp.131~188 (1995), 고려의학
  3. 안세영, "갑상선클리닉", pp.132~154 (2004), 성보사
  4. Wiersinga, W.M, "Thyroid hormone replacement therapy", Horm Res, 56(1), pp.74~81 (2001)
  5. Das, K, "Modulation of rat liver mitochondrial antioxidant defence system by thyroid hormone", Biochim Biophys Acta, 1537(1), pp.1~13 (2001)
  6. Subudhi, U, "Supplementation of curcumin and vitamin E enhances oxidative stress, but restores hepatic histoarchitecture in hypothyroid rats", Life Sci, 84(11), pp.372~379 (2009)
  7. Sarandöl, E, "Oxidative stress and serum paraoxonase activity in experimental hypothyroidism: effect of vitamin E supplementation", Cell Biochem Funct, 23(1), pp.1~8 (2005)
  8. O'Connor, J.C, "Evaluation of a 15-day screening assay using intact male rats for identifying steroid biosynthesis inhibitors and thyroid modulators", Toxicol Sci, 69(1), pp.79~91 (2002)
  9. 杜鎬京, "東醫腎系學", pp.867~874 (1993), 東洋醫學硏究院
  10. 김인락, "本草學", pp.240~242 (2007), 圖書出版 永林社
  11. Yip, E.C, "Protocatechuic acid induces cell death in HepG2 hepatocellular carcinoma cells through a c-Jun N-terminal kinase-dependent mechanism", Cell Biol Toxicol, 22(4), pp.293~302 (2006)
  12. Ko, H.C, "The effect of medicinal plants used in Chinese folk medicine on RANTES secretion by virus-infected human epithelial cells", J Ethnopharmacol, 107(2), pp.205~210 (2006)
  13. 박선관, "사염화탄소 유발 간독성에 대한 금은화의 작용", 응용약물학회지, 10(4), pp.32~36 (2002), 한국응용약물학회
  14. 이정노, "수용성 금은화 추출물이 Trimellitic Anhydride 유도 마우스 접촉성 과민반응에 미치는 영향", 대한본초학회지, 23(2), pp.51~58 (2008), 대한본초학회
  15. 배지현, "식중독 유발세균의 증식에 미치는 금은화 추출물의 항균효과", 한국식품과학회지, 37(4), pp.642~647 (2005), 한국식품과학회
  16. Suh, S.J, "The naturally occurring biflavonoid, ochnaflavone, inhibits LPS-induced iNOS expression, which is mediated by ERK1", 2 via NF-kappaB regulation, in RAW264.7 cells, 2006, pp.447(2), 136~146
  17. Luo, Z.H, "The combined modulating effects of cerium nitrate with certain Chinese traditional drugs on altered cell-mediated immunities in scald mice", Zhonghua Wai Ke Za Zhi, 9, pp.562~565 (1990)
  18. Chang, W.C, "Inhibition of platelet activation and endothelial cell injury by polyphenolic compounds isolated from Lonicera japonica Thunb", Prostaglandins Leukot Essent Fatty Acids, 45(4), pp.307~312 (1992)
  19. Chun-Whan Choi, "Antioxidant Constituents and a New Triterpenoid Glycoside from Flos Lonicerae", Archives of Pharmacal Research, 30(1), pp.1~7 (2007), 대한약학회
  20. Kavutcu, M, "Reduced enzymatic antioxidant defense mechanism in kidney tissues from gentamicin-treated guinea pigs", effects of vitamins E and C. Nephron, 72(2), pp.269~274 (1996)
  21. Lowry, O.H, "Protein measurement with the Folin phenol reagent", J Biol Chem, 193(1), pp.265~275 (1951)
  22. Jamall, I.S, "Effects of cadmium on glutathione peroxidase, superoxidase dismutase and lipid peroxidation in the rat heart: a possible mechanism of cadmium cardiotoxicity", Toxicol Appl Pharmacol, 80(1), pp.33~42 (1985)
  23. Pick, E, "Superoxide anion and hydrogen peroxide production by chemically elicited peritoneal macrophages--induction by multiple nonphagocytic stimuli", Cell Immunol, 59(2), pp.301~318 (1981)
  24. Aebi, H. Catalase, "Methods in Enzymatic Analysis", pp.673~686 (1974), Academic Press Inc
  25. Nishikimi, M, "The occurrence of superoxide anion in the reaction of reduced PMS and molecular oxygen", Biochem Biophys Res Comm, 46(2), pp.849~854 (1972)
  26. Kakkar, P, "Modified spectrophotometric assay of SOD", Indian J Biochem Biophys, 21, pp.130~132 (1984)
  27. Andreoli, T.E, "Cecil Essentials of Medicine", pp.436~437 (1986), WB Saunder’s Company
  28. Guyton, A.C, "Textbook of Medical Physiology", pp.906~908 (1986), WB Saunder’s Company
  29. Roti, E, "The use and misuse of thyroid hormone", Endocr Rev, 14(4), pp.401~424 (1993)
  30. 김민선, "렛트에서 단기간의 갑상선 기능 변화에 따른 혈중 렙틴 농도의 변화", 대한내분비학회지, 17(2), pp.197~205 (2002), 대한내분비학회
  31. Devdhar, M, "Hypothyroidism", Endocrinol Metab Clin North Am, 36(3), pp.595~615 (2007)
  32. Dory, L, "Rat plasma lipoproteins and apolipoproteins in experimental hypothyroidism", J Lipid Res, 22(2), pp.287~296 (1981)
  33. Frost, P.H, "Rationale for use of non-high-density lipoprotein cholesterol rather than low-density lipoprotein cholesterol as a tool for lipoprotein cholesterol screening and assessment of risk and therapy", Am J Cardiol, 81(4A), pp.26B~31B (1998)
  34. Teixeira, P.D.F, "Lipid profile in different degrees of hypothyroidism and effects of levothyroxine replacement in mild thyroid failure", Transl Res, 151(4), pp.224~231 (2008)
  35. Feng, X, "Thyroid hormone regulation of hepatic genes in vivo detected by complementary DNA microarray", Mol Endocrinol, 14(7), pp.947~955 (2000)
  36. Laycock, M.A, "The neuromuscular effects of hypothyroidism", Semin Neurol, 11(3), pp.288~294 (1991)
  37. Amin, A, "Oxidative stress mediates drug-induced hepatotoxicity in rats: a possible role of DNA fragmentation", Toxicology, 208(3), pp.367~375 (2005)
  38. Chattopadhyay, S, "Differential expression profiles of antioxidant enzymes and glutathione redox status in hyperthyroid rats: a temporal analysis", Comp Biochem Physiol C Toxicol Pharmacol, 146(3), pp.383~391 (2007)
  39. Williams, K.V, "Fifty years of experience with propylthiouracil-associated hepatotoxicity: what have we learned?", J Clin Endocrinol Metab, 82(6), pp.1727~1733 (1997)

관련 논문AI추천

이 간행물의 다른 논문 (16건)