香砂養胃湯이 PTU로 유발된 Rat의 갑상선기능저하와 간손상에 미치는 영향
Effects of Hyangsayangyi-tang Aqueous Extracts on the Hypothyroidism Related Hepatic Damages induced by PTU in Rats
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To evaluate the benefits of Hyangsayangyi-tang aqueous extracts (HSYYT) on the propylthiouracil (PTU)-induced Rat hypothyroidism. 6 groups, each consisting 8 Rats were used in the present study – Intact vehicle control, PTU control, LT4, HSYYT 500, 250 and 125 ㎎/㎏ treated groups. HSYYT was given, once day for 42 days from 2 weeks before start of PTU treatment as an oral dose of 500, 250 and 125 ㎎/㎏(body weight), and for 28 days while the PTU 10 ㎎/㎏ by daily subcutaneous treatment induced hypothyroidism. Compared the results with LT4 0.5 ㎎/㎏ intraperitoneally treated rats in this experiment. Results of the PTU treatment included; decreases of body weight, increase in thyroid weight, decrease in liver weight, in serum T3, and T4 level decrease with increase of serum TSH levels, in serum HDL increase and in TG content decrease, decrease in liver antioxidants defense system, increase of serum AST levels were observed. However, these PTU induced hypothyroidism and related hepatic damages were dose-dependently inhibited by treatment of HSYYT 500 and 250 ㎎/㎏, and they also effectively regulated the PTU-induced abnormal antioxidant defense system changes in liver. Therefore, in comparison with the PTU control group, it was effective and advantageous changes were not observed in HSYYT 125 ㎎/㎏ treated rats on the PTU induced hypothyroidism and related hepatic damages. In this experiment, HSYYT 500 and 250 ㎎/㎏ dose-dependently inhibited PTU-induced hypothyroidism and related liver damage in rats but not in HSYYT 125 ㎎/㎏.
Abstract
To evaluate the benefits of Hyangsayangyi-tang aqueous extracts (HSYYT) on the propylthiouracil (PTU)-induced Rat hypothyroidism. 6 groups, each consisting 8 Rats were used in the present study – Intact vehicle control, PTU control, LT4, HSYYT 500, 250 and 125 ㎎/㎏ treated groups. HSYYT was given, once day for 42 days from 2 weeks before start of PTU treatment as an oral dose of 500, 250 and 125 ㎎/㎏(body weight), and for 28 days while the PTU 10 ㎎/㎏ by daily subcutaneous treatment induced hypothyroidism. Compared the results with LT4 0.5 ㎎/㎏ intraperitoneally treated rats in this experiment. Results of the PTU treatment included; decreases of body weight, increase in thyroid weight, decrease in liver weight, in serum T3, and T4 level decrease with increase of serum TSH levels, in serum HDL increase and in TG content decrease, decrease in liver antioxidants defense system, increase of serum AST levels were observed. However, these PTU induced hypothyroidism and related hepatic damages were dose-dependently inhibited by treatment of HSYYT 500 and 250 ㎎/㎏, and they also effectively regulated the PTU-induced abnormal antioxidant defense system changes in liver. Therefore, in comparison with the PTU control group, it was effective and advantageous changes were not observed in HSYYT 125 ㎎/㎏ treated rats on the PTU induced hypothyroidism and related hepatic damages. In this experiment, HSYYT 500 and 250 ㎎/㎏ dose-dependently inhibited PTU-induced hypothyroidism and related liver damage in rats but not in HSYYT 125 ㎎/㎏.
참고문헌 (30)
- Zo, B.Y., "Clinical Thyroidology", pp.437~476 (2010), Korea Medical Book Publisher
- Guyton, A. C, "Textbook of Medical Physiology", pp.906~908 (1986), WB Saunder's Company
- Ahn, S. Y, "Thyroid Clinic", pp.132~154 (2004), Seoungbosa
- Wiersinga, W.M., "Thyroid hormone replacement therapy", Horm Res, 56, pp.74~81 (2001)
- Subudhi, U., "Supplementation of curcumin and vitamin E enhances oxidative stress, but restores hepatic histoarchitecture in hypothyroid rats", Life Sci, 84, pp.372~379 (2009)
- Das, K., "Modulation of rat liver mitochondrial antioxidant defence system by thyroid hormone", Biochim Biophys Acta, 1537, pp.1~13 (2001)
- Feng, X., "Thyroid hormone regulation of hepatic genes in vivo detected by complementary DNA microarray.", Mol Endocrinol, 14, pp.947~955 (2000)
- Simon-Giavarotti, K. A., "Enhancement of lindane-induced liver oxidative stress and hepatotoxicity by thyroid hormone is reduced by gadolinium chloride", Free Radic Res, 36, pp.1033~1039 (2002)
- Sarandöl, E., "Oxidative stress and serum paraoxonase activity in experimental hypothyroidism: effect of vitamin E supplementation", Cell Biochem Funct, 23, pp.1~8 (2005)
- 구세광, "금은화가 6-n-propyl-2-thiouracil (PTU) 로 유발된 흰쥐의 갑상샘 기능저하증에 미치는 영향", 동의생리병리학회지, 24(4), pp.630~637 (2010), 한의병리학회
- 김승모, "시호가 PTU (6-n-propyl-2-thiouracil)로 유발된 Rat 갑상샘 기능저하증에 미치는 영향", 동의생리병리학회지, 26(5), pp.714~723 (2012), 한의병리학회
- Bhanja, S., "Modulation of antioxidant enzyme expression by PTU-induced hypothyroidism in cerebral cortex of postnatal rat brain", Neurochem Res, 38, pp.42~49 (2013)
- Rayman, M. P., "Randomized controlled trial of the effect of selenium supplementation on thyroid function in the elderly in the United Kingdom", Am J Clin Nutr, 87, pp.370~378 (2008)
- 최홍식, "香砂養胃湯이 Propylthiouracil로 유발된 Rat 갑상샘기능저하성 웅성 생식기 손상에 미치는 영향", 대한본초학회지, 28(6), pp.59~70 (2013), 대한본초학회
- 최병철, "香砂養胃湯 전탕액이 노화쥐의 비장, 췌장, 위장 세포의 항산화능에 미치는 영향", 사상체질의학회지, 20(2), pp.72~84 (2008), 사상체질의학회
- Hong, N. D., "Studies on the Efficacy of Combined Preparations of Crude Drug(XXXIX). -Effect of Hyangsayangwee-Tang on the Stomach and Intestinal Disorder-", Korean J Pharmacogn, 20, pp.188~195 (1989)
- Kakkar, P., "Modified spectrophotometric assay of SOD", India J Biochem Biophys, 2, pp.130~132 (1984)
- Levene, A., "Pathological factors influencing excision of tumours in the head and neck. Part I.", Part I. Clin Otalary, 6, pp.145~151 (1981)
- Ludbrook, J., "Update: microcomputer statistics packages. A personal view", Clin Exp Pharmacol Physiol, 24, pp.294~296 (1997)
- Doo, H. K, "Oriental Medicine of Nephrology", pp.729~1065 (1993), The Academy of oriental Medicine
- Roti, E., "T he use and misuse of thyroid hormone", Endocr Rev, 14, pp.401~424 (1993)
- 김민선, "렛트에서 단기간의 갑상선 기능 변화에 따른 혈중 렙틴 농도의 변화", Endocrinology and Metabolism, 17(2), pp.197~205 (2002), 대한내분비학회
- O'Connor, J. C., "Evaluation of a 15-day screening assay using intact male rats for identifying steroid biosynthesis inhibitors and thyriod modulators", Toxicol Sci, 69, pp.79~91 (2002)
- Yang, Y., "Regulated hypothermia in the hypothyroid rat induced by administration of propylthiouracil", Am J Physiol, 272, pp.R1390~R1395 (1997)
- Oner, J., "Effect of vitamin E on follicular cell proliferation and expression of apoptosis-associated factors in rats with 6-N-propyl-2-thiouracil-induced goitrogenesis", Folia Histochem Cytobiol, 41, pp.213~217 (2003)
- Devdhar, M., "Hypothyroidism", Endocrinol Metab Clin North Am, 36, pp.595~615 (2007)
- Teixeira, P. D. F., "Lipid profile in different degrees of hypothyroidism and effects of levothyroxine replacement in mild thyroid failure", Transl Res, 151, pp.224~231 (2008)
- Laycock, M. A., "The neuromuscular effects of hyperthyrodism", Semin Neurol, 11, pp.288~294 (1991)
- Williams, K. V., "Fifty years of experience with propylthiouracil-associated hepatotoxicity: what have we learned?", J Clin Endocrinol Metab, 82, pp.1727~1733 (1997)
- Bhanja, S., "PTU-induced hypothyroidism modulates antioxidant defense status in the developing cerebellum", Int J Dev Neurosci, 28, pp.251~262 (2010)