소아 설사환자에서 분리된 장병원성 대장균의 분자생물학적 특성
Phenotypic and Genotypic Characteristics of Enteropathogenic E. coli Isolated from Children with Diarrhea in Gwangju City
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Background : Enteropathgenic Escherichia coli (EPEC) commonly causes infantile diarrhea in the developing countries. This study aims to find out the phenotypic and genotypic characteristics of EPEC in children with diarrhea in Gwangju city. Methods : We isolated 35 strains from the stool obtained from diarrheal patients and investigated the presence of various virulence genes, adherence patterns, hemolysis, and antibiotic resistance patterns. Results : All isolates were negative for the EPEC adherence factor (EAF) plasmid, and 14 isolates were bfpA-positive by PCR. The eae, tir, espA, and espB genes were analyzed by multiplex PCR. When the results of the four multiplex PCRs were analysed, we observed that the rate of the presence of eaeγ-tirα-espAα-espBα was highest. The incidence of enteroaggregative E. coli heat- stable enterotoxin (east) was 17.1%. Analysis of Hep-2 cell adherence showed three adherence patterns : the localized adherence pattern, the diffuse adherence pattern, the localized adherence-like pattern. In hemolysin assay, four isolates produced enterohemolysin. The resistance rate of isolates against tetracycline, streptomycin, ampicillin, and rifampin was 56%, 39%, 34%, and 34%, respectively. All isolates were susceptible to ciprofloxacin and colistin. Conclusion : In our study, the rate of the presence of eaeγ-tirα-espAα-espBα was the highest. Analysis of Hep-2 cell adherence showed various adherence patterns. Seventy-five percent of isolates were resistant to at least one antibiotic and 28% were resistant to four or more antibiotics.
Abstract
Background : Enteropathgenic Escherichia coli (EPEC) commonly causes infantile diarrhea in the developing countries. This study aims to find out the phenotypic and genotypic characteristics of EPEC in children with diarrhea in Gwangju city. Methods : We isolated 35 strains from the stool obtained from diarrheal patients and investigated the presence of various virulence genes, adherence patterns, hemolysis, and antibiotic resistance patterns. Results : All isolates were negative for the EPEC adherence factor (EAF) plasmid, and 14 isolates were bfpA-positive by PCR. The eae, tir, espA, and espB genes were analyzed by multiplex PCR. When the results of the four multiplex PCRs were analysed, we observed that the rate of the presence of eaeγ-tirα-espAα-espBα was highest. The incidence of enteroaggregative E. coli heat- stable enterotoxin (east) was 17.1%. Analysis of Hep-2 cell adherence showed three adherence patterns : the localized adherence pattern, the diffuse adherence pattern, the localized adherence-like pattern. In hemolysin assay, four isolates produced enterohemolysin. The resistance rate of isolates against tetracycline, streptomycin, ampicillin, and rifampin was 56%, 39%, 34%, and 34%, respectively. All isolates were susceptible to ciprofloxacin and colistin. Conclusion : In our study, the rate of the presence of eaeγ-tirα-espAα-espBα was the highest. Analysis of Hep-2 cell adherence showed various adherence patterns. Seventy-five percent of isolates were resistant to at least one antibiotic and 28% were resistant to four or more antibiotics.
참고문헌 (40)
- "Diarrheagenic Escherichia coli", 11, pp.142~201 (1998)
- "Manual for laboratory investigations for acute enteric infections" (1997)
- "Distinctive patterns of adherence of enteropathogenic Escherichia coli to HeLa cells", 45, pp.534~6 (1984)
- "Plasmid mediated adhesion of enteropathogenic Escherichia coli", 2, pp.534~53 (1983)
- "An inducible bundle-forming pilus of enteropathogenic Escherichia coli", 254, pp.710~3 (1991)
- "Enteropathogenic and enterohaemorrhagic Escherichia coli:more subversive elements", 30, pp.911~921 (1998)
- "Single multiplex polymerase chain reaction to detect diverse loci associated with diarrheagenic Escherichia coli", 9, pp.127~31 (2003)
- "Comparison of eae,tir,espA and espB genes of bovine and human attaching and effacing Escherichia coli by multiplex polymerase chain reaction", 178, pp.177~82 (1999)
- "Genotypic characterization of enteropathogenic Escherichia coli isolated in Belgium from dogs and cats", 151, pp.865~71 (2000)
- "Traditional enteropathogenic Escherichia coli of infantile diarrhea", 9, pp.28~53 (1987)
- "Epidemic gastroenteritis of infant in Aberdeen during 1947", 24, pp.45~53 (1949)
- "Properties of vero cytotoxin-producing Escherichia coli of human origin of O serogroups other than O157", 166, pp.797~802 (1992)
- "Enteropathogenic Escherichia coli of classic serotypes associated with infant diarrhea:epidemiology and pathogenesis", 6, pp.31~51 (1984)
- "Infections of the gastrointestinal tract", pp.709~26 (1995), Raven Press
- "Failure to detect conventional enterotoxins in classical enteropathogenic(serotyped)Escherichia coli strains of proven pathogenicity", 38, pp.798~801 (1982)
- "Risk of diarrhea during the first year of life associated with initial and subsequent colonization by specific enteropathogens", 131, pp.886~904 (1990)
- "Prevalence of Escherichia coli strains with localized,diffuse,and aggregative adherence to HeLa cells in infants with diarrhea and matched controls", 27, pp.266~9 (1989)
- "Enteropathogenic associated with acute diarrheal disease in urban infants in Sao Paulo,Brazil", 164, pp.331~7 (1991)
- "Summer diarrhoea in African infants and children", 55, pp.923~8 (1980)
- "Typical and atypical enteropathogenic Escherichia coli", 8, pp.508~13 (2002)
- "A plasmid-encoded regulatory region activates chromosomal eaeA expression in enteropathogenic Escherichia coli", 63, pp.1767~76 (1995)
- "Escherichia coli O128 strains from infants with diarrhea commonly show localized adhesion and positivity in the fluorescent-actin staining test but do not hybridize with an enteropathogenic E.coli adherence factor probe", 59, pp.1569~71 (1991)
- "Genetic and phenotypic analysis of Escherichia coli with enteropathogenic characteristics isolated from Seattle children", 175, pp.1382~9 (1997)
- "Molecular characterization of strains of enteroinvasive Escherichia coli O143 including isolates from a large outbreak in Houston", pp.889~93 (1992)
- "Use of DNA probes and HEp-2 cell adherence assay to detect diarrheagenic Escherichia coli", 158, pp.224~28 (1988)
- "Attaching and effacing enteropathogenic Escherichia coli as a cause of infantile diarrhea in Bangkok", 164, pp.550~4 (1991)
- "The diarrheal response of humans to some classic serotypes of enteropathogenic Escherichia coli is dependent on a plasmid encoding an enteroadherensiveness factor", 152, pp.550~9 (1985)
- "Outbreak of diarrhoea due to Escherichia coli O111 B4 in school children and adults association of Vi antigen-like reactivity", pp.831~336 4 (1990)
- "Use of gene probes and adhesion tests to characterize Escherichia coli belonging to enteropathogenic serogroups isolated in the United Kingdom", 44, pp.438~43 (1996)
- "Virulence determinants in nontoxinogenic Escherichia coli O157 strains that cause infantile diarrhea", 61, pp.4894~8 (1993)
- "Identification of a family of intimins common to Escherichia coli causing attaching-effacing lesions in rabbits,humans,and swine", 65, pp.320~6 (1997)
- "Detection of intimins α four intimin derivatives expressed by attaching and effacing microbial pathogens", 36, pp.662~8 (1998), Bain C
- "Typing of intimin genes in human and animal enterohemorrhagic and enteropathogenic Escherichia coli:characterization of a new intimin variant", 68, pp.64~71 (2000)
- "Phenotypic and genotypic characteristics of Escherichia coli strains of non-enteropathogenic E serogroups that carry eae and lack the EPEC adherence factor and Shiga toxin DNA probe sequences", 183, pp.762~72 (2001)
- "Genotypic and phenotypic characterization of attaching and effacing Escherichia coli isolated from children with and without diarrhoea in Londrina", 52(6), pp.499~504 (2003)
- "Subtyping intimin genes from enteropathogenic Escherichia coli associated with outbreaks and sporadic cases in the United Kingdom and Eire", 17, pp.149~156 (2003)
- "Subtypes of intimin among non-toxigenic Escherichia coli from diarrheic calves in Brazil", 64, pp.15~20 (2000)
- "An outbreak of foodborne illness caused by Escherichia coli O39 an agent not fitting into the existing scheme for classifying diarrheogenic E", 176, pp.1625~8 (1997)
- "Distribution and expression of the astA gene(EAST1 toxin)in Escherichia coli and Salmonella", 291, pp.15~20 (2001)
- "IS1414,an Escherichia coli insertion sequence with a heat- stable enterotoxin gene embedded in a transposase-like gene", 68, pp.5710~5 (2000)
