Virulence genes and antimicrobial resistance of pathogenic Escherichia coli isolated from diarrheic weaned piglets in Korea
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For efficient prevention and treatment of enteric colibacillosis, understanding about latest virulence factors and antimicrobial resistance of Escherichia coli is essentially needed. The aim of this study was to survey antimicrobial resistance and determine the prevalence of fimbriae and enterotoxin genes among 118 pathogenic E. coli isolates obtained from Korean pigs with diarrhea between 2016 and 2017. The genes for the toxins and adhesins were amplified by polymerase chain reaction (PCR). The susceptibility of the E. coli isolates to antimicrobials were tested using the standard Kirby-Bauer disk diffusion method. The most prevalent fimbrial antigen was F18 (40.7%), followed by F4 (16.9%), and the most prevalent combinations of toxin genes were Stx2e (21.2%), STb:EAST-1 (19.5%), and STa:STb (16.9%), respectively. Among the pathotypes, enterotoxigenic E. coli (ETEC) was the most predominant (67.8%), followed by Shiga-toxin producing E. coli (STEC, 23.7%). We confirmed high resistance rates to chloramphenicol (88.1%), tetracycline (86.4%), streptomycin (86.4%), and ampicillin (86.4%). And the majorities of isolates (90.7%) showed multi-drug resistance which means having resistance to 3 or more subclasses of antimicrobials. Results of this study can be a source of valuable data for investigating the epidemiology of and control measures for enteric colibacillosis in Korean piggeries.
Abstract
For efficient prevention and treatment of enteric colibacillosis, understanding about latest virulence factors and antimicrobial resistance of Escherichia coli is essentially needed. The aim of this study was to survey antimicrobial resistance and determine the prevalence of fimbriae and enterotoxin genes among 118 pathogenic E. coli isolates obtained from Korean pigs with diarrhea between 2016 and 2017. The genes for the toxins and adhesins were amplified by polymerase chain reaction (PCR). The susceptibility of the E. coli isolates to antimicrobials were tested using the standard Kirby-Bauer disk diffusion method. The most prevalent fimbrial antigen was F18 (40.7%), followed by F4 (16.9%), and the most prevalent combinations of toxin genes were Stx2e (21.2%), STb:EAST-1 (19.5%), and STa:STb (16.9%), respectively. Among the pathotypes, enterotoxigenic E. coli (ETEC) was the most predominant (67.8%), followed by Shiga-toxin producing E. coli (STEC, 23.7%). We confirmed high resistance rates to chloramphenicol (88.1%), tetracycline (86.4%), streptomycin (86.4%), and ampicillin (86.4%). And the majorities of isolates (90.7%) showed multi-drug resistance which means having resistance to 3 or more subclasses of antimicrobials. Results of this study can be a source of valuable data for investigating the epidemiology of and control measures for enteric colibacillosis in Korean piggeries.
참고문헌 (29)
- Fairbrother JM, "Diseases of swine", pp.723~749 (2012), John Wiley Sons
- Byun JW, "O-serogroups, virulence genes of pathogenic Escherichia coli and pulsed-field gel electrophoresis (PFGE) patterns of O149 isolates from diarrhoeic piglets in Korea", Vet Med (Praha), 58, pp.468~476 (2013)
- Duan Q, "Major virulence factors of enterotoxigenic Escherichia coli in pigs", Ann Microbiol, 62, pp.7~14 (2012)
- National Veterinary Research & Quarantine Service, "Establishment of control system of antibiotics for livestocks" (2006), Ministry of Food and Drug Safety
- Animal and Plant Quarantine Agency, "Establishment of antimicrobial resistance surveillance system for livestock 2012" (2013), Ministry of Agriculture, Food and Rural Affairs
- National Food Institute, "DANMAP 2013 - Use of antimicrobial agents and occurrence of antimicrobial resistance in bacteria from food animals, food and humans in Denmark" (2013), DANMAP-Danish Integrated Antimicrobial Resistance Monitoring and Research Programme
- Report on the Japanese veterinary antimicrobials resistance monitoring system 2012 to 2013, "Japanese Veterinary Antimicrobial Resistance Monitoring System", National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries, ~ (2016)
- Canadian integrated program for antimicrobial resistance surveillance (CIPARS) 2014 Annual Report, "Government of Canada", Public Health Agency of Canada, ~ (2016)
- 도경효, "Prevalence of O-serogroups, virulence genes, and F18 antigenic variants in Escherichia coli isolated from weaned piglets with diarrhea in Korea during 2008–2016", Journal of Veterinary Science, 20(1), pp.43~50 (2019), 대한수의학회[DOI]
- 임숙경, "건강한 가축에서 분리한 Escherichia coli의 항생제 내성 조사(2010~2012년)", 대한수의학회지, 54(3), pp.131~137 (2014), 대한수의학회[DOI]
- Bauer AW, "Antibiotic susceptibility testing by a standardized single disk method", Am J Clin Pathol, 45, pp.493~496 (1966)
- Clinical & Laboratory Standards Institute, "M100-S24: Performance standards for antimicrobial susceptibility testing; twenty-fourth informational supplement", Clinical &Laboratory Standards Institute, ~ (2014)
- Magiorakos AP, "Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria : an international expert proposal for interim standard definitions for acquired resistance", Clin Microbiol Infect, 18, pp.268~281 (2012)
- Fairbrother JM, "Escherichia coli in postweaning diarrhea in pigs : an update on bacterial types, pathogenesis, and prevention strategies", Anim Health Res Rev, 6, pp.17~39 (2005)
- Kwon D, "Genotypic prevalence of the fimbrial adhesins(F4, F5, F6, F41 and F18)and toxins(LT, STa, STb and Sbc2e)in Escherichia coli isolated from postweaning pigs with diarrhoea or oedema disease in Korea", Vet Rec, 150, pp.35~37 (2002)
- Kwon D, "Prevalence of genotypes for fimbriae and enterotoxins and of O serogroups in Escherichia coli isolated from diarrheic piglets in Korea", J Vet Diagn Invest, 11, pp.146~151 (1999)
- Lee SI, "Genotypes, antibiogram, and pulsed-field gel electrophoresis profiles of Escherichia coli strains from piglets in Korea", J Vet Diagn Invest, 21, pp.510~516 (2009)
- Chae MJ, "Virulence genes of Escherichia coli isolates from piglets with diarrhea in Korea", J Anim Vet Adv, 11, pp.9~12 (2012)
- Ngeleka M, "Isolation and association of Escherichia coli AIDA-I", STb, rather than EAST1 pathotype, with diarrhea in piglets and antibiotic sensitivity of isolates, 2003, pp.15, 242~252
- Nguyen UV, "High susceptibility prevalence for F4+ and F18+ Escherichia coli in Flemish pigs", Vet Microbiol, 202, pp.52~57 (2017)
- Niewerth U, "The AIDA autotransporter system is associated with F18 and Stx2e in Escherichia coli isolates from pigs diagnosed with edema disease and postweaning diarrhea", Clin Diagn Lab Immunol, 8, pp.143~149 (2001)
- Zhang W, "Prevalence of virulence genes in Escherichia coli strains recently isolated from young pigs with diarrhea in the US", Vet Microbiol, 123, pp.145~152 (2007)
- Zhao L, "Analysis of the AIDA-I gene sequence and prevalence in Escherichia coli isolates from pigs with post-weaning diarrhoea and oedema disease", Vet J, 180, pp.124~129 (2009)
- Leclerc S, "Paa, originally identified in attaching and effacing Escherichia coli, is also associated with enterotoxigenic E. coli", Res Microbiol, 158, pp.97~104 (2007)
- Alonso CA, "Occurrence and characterization of stx and", or eae-positive Escherichia coli isolated from wildlife, including a typical EPEC strain from a wild boar, 2017, pp.207, 69~73
- Sato JPH, "Virulence profiles of enterotoxigenic Escherichia coli isolated from piglets with post-weaning diarrhea and classification according to fecal consistency", Pesq Vet Bras, 36, pp.253~257 (2016)
- Cho JK, "Antimicrobial drug resistance of Escherichia coli isolated from cattle, swine and chicken", Korean J Vet Public Health, 30, pp.9~18 (2006)
- Do KH, "Serogroups, virulence genes and antimicrobial resistance of F4+and F18+ Escherichia coli isolated from weaned piglets", Pak Vet J, 39, pp.266~270 (2019)
- 도경효, "Antimicrobial resistance of Stx2e positive Escherichia coli before and after ban on antibiotic growth promoters", Journal of Biomedical and Translational Research, 18(3), pp.84~92 (2017), 동물의학연구소